Multifunctions of Pleurotus sajor-caju (Fr.) Singer: A highly nutritious food and a source for bioactive compounds

Finimundy, Tiane C.; Barros, Lillian; Calhelha, Ricardo C.; Prieto, Miguel A.; Abreu, Rui M.V.; Dillon, Aldo José Pinheiro; Henriques, João Antônio Pêgas; Roesch‐Ely, Mariana; Ferreira, Isabel C.F.R.

doi:10.1016/j.foodchem.2017.10.088

Cited by 30 publications

(28 citation statements)

References 34 publications

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“…Therefore, the compound would not be deemed completely inactive, in keeping with its weak estrogenic activity in mouse bioassays [113] and human breast cancer cell lines [114]. Moreover, the general notion of 4OH as a ligand in complex with proteins should not be surprising, given that it has been docked into human cyclooxygenase-2 (COX-2) [115], despite negative results in a COX-2 dependent human smooth muscle cell assay [116]. Furthermore, 4OH is found as a bound ligand in a variety of enzymes, including carbonic anhydrase [117], p -hydroxybenzoate hydroxylase [118], and 4-hydroxybenzoate octaprenyltransferase [119].…”

Section: Discussionmentioning

confidence: 99%

Homology models of mouse and rat estrogen receptor-α ligand-binding domain created by in silico mutagenesis of a human template: Molecular docking with 17β-estradiol, diethylstilbestrol, and paraben analogs

Gonzalez

Rae

Colacino

et al. 2019

Computational Toxicology

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Crystal structures exist for human, but not rodent, estrogen receptor-α ligand-binding domain (ERα-LBD). Consequently, rodent studies involving binding of compounds to ERα-LBD are limited in their molecular-level interpretation and extrapolation to humans. Because the sequences of rodent and human ERα-LBDs are > 95% identical, we expected their 3D structures and ligand binding to be highly similar. To test this hypothesis, we used the human ERα-LBD structure (PDB 3UUD) as a template to produce rat and mouse homology models. Employing the rodent models and human structure, we generated docking poses of 23 Group A ligands (17ß-estradiol, diethylstilbestrol, and 21 paraben analogs) in AutoDock Vina for interspecies comparisons. Ligand RMSDs (Å) (median, 95% CI) were 0.49 (0.21–1.82) (human-mouse) and 1.19 (0.22–1.82) (human-rat), well below the 2.0–2.5 Å range for equivalent docking poses. Numbers of interspecies ligand-receptor residue contacts were highly similar, with Sorensen Sc (%) = 96.8 (90.0–100) (human-mouse) and 97.7 (89.5–100) (human-rat). Likewise, numbers of interspecies ligand-receptor residue contacts were highly correlated: Pearson r = 0.913 (human-mouse) and 0.925 (human-rat). Numbers of interspecies ligand-receptor atom contacts were even more tightly correlated: r = 0.979 (human-mouse) and 0.986 (human-rat). Pyramid plots of numbers of ligand-receptor atom contacts by residue exhibited high interspecies symmetry and had Spearman rs = 0.977 (human-mouse) and 0.966 (human-rat). Group B ligands included 15 ring-substituted parabens recently shown experimentally to exhibit decreased binding to human ERα and to exert increased antimicrobial activity. Ligand efficiencies calculated from docking ligands into human ERα-LBD were well correlated with those derived from published experimental data (Pearson partial rp = 0.894 and 0.918; Groups A and B, respectively). Overall, the results indicate that our constructed rodent ERα-LBDs interact with ligands in like manner to the human receptor, thus providing a high level of confidence in extrapolations of rodent to human ligand-receptor interactions.

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Section: Discussionmentioning

confidence: 99%

Homology models of mouse and rat estrogen receptor-α ligand-binding domain created by in silico mutagenesis of a human template: Molecular docking with 17β-estradiol, diethylstilbestrol, and paraben analogs

Gonzalez

Rae

Colacino

et al. 2019

Computational Toxicology

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“…Cytotoxicity assay of the fungal fruiting body extract demonstrated strong inhibition against growth of non-small cell lung carcinoma (NCI-H460), breast (MCF-7) and cervical (HeLa) carcinoma cells. Pleurotus sajor-caju extract does not display cytotoxicity against non-tumour cells (primary PLP2) even at a test concentration of 400 µg/mL [72]. Ethanol extract of mycelia of the oyster mushroom, P. ostreatus, demonstrated a broad-spectrum antibacterial activity.…”

Section: Antistaphylococcal Natural Products From Basidiomycetesmentioning

confidence: 96%

“…Fruiting body extracts of Pleurotus sajor-caju (Fr.) Singer exhibited some extent of antistaphylococcal activity against MSSA and MRSA with a MIC value of 10 mg/mL with the presence of compounds such as p-hydroxybenzoic acid 7, p-Coumaric acid (8) and cinnamic acid (9) [72,73]. Cytotoxicity assay of the fungal fruiting body extract demonstrated strong inhibition against growth of non-small cell lung carcinoma (NCI-H460), breast (MCF-7) and cervical (HeLa) carcinoma cells.…”

Section: Antistaphylococcal Natural Products From Basidiomycetesmentioning

confidence: 99%

“…Ethanolic extract of Pleurotus sajur-caju displayed antimicrobial activity against P. aeruginosa and Klebsiella pneumoniae while the methanolic extract was active against E. coli [94,95]. Pleurotus sajor-caju ethanolic extract is composed of lipophilic compounds, p-Hydroxybenzoic 7, p-coumaric (8) and cinnamic acids (9) at respective concentration: 66 ± 4, 43.7 ± 0.9 and 37.5 ± 0.3 µg/g extract [72]. A previous report mentioned that the most suitable solvent to extract maximum antimicrobial bioactive compounds from the fungus is ethanol [94].…”

Section: Antibacterial Natural Products From Basidiomycetes Against Omentioning

confidence: 99%

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A Review on Antistaphylococcal Secondary Metabolites from Basidiomycetes

et al. 2020

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Fungi are a rich source of secondary metabolites with several pharmacological activities such as antifungal, antioxidant, antibacterial and anticancer to name a few. Due to the large number of diverse structured chemical compounds they produce, fungi from the phyla Ascomycota, Basidiomycota and Muccoromycota have been intensively studied for isolation of bioactive compounds. Basidiomycetes-derived secondary metabolites are known as a promising source of antibacterial compounds with activity against Gram-positive bacteria. The continued emergence of antimicrobial resistance (AMR) poses a major challenge to patient health as it leads to higher morbidity and mortality, higher hospital-stay duration and substantial economic burden in global healthcare sector. One of the key culprits for AMR crisis is Staphylococcus aureus causing community-acquired infections as the pathogen develops resistance towards multiple antibiotics. The recent emergence of community strains of S. aureus harbouring methicillin-resistant (MRSA), vancomycin-intermediate (VISA) and vancomycin-resistant (VRSA) genes associated with increased virulence is challenging. Despite the few significant developments in antibiotic research, successful MRSA therapeutic options are still needed to reduce the use of scanty and expensive second-line treatments. This paper provides an overview of findings from various studies on antibacterial secondary metabolites from basidiomycetes, with a special focus on antistaphylococcal activity.

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“…An increase in the ratio of bone resorption to bone formation causes low bone mass and strength, which leads to posseses various biological activities, including antimicroorganisms, antitumor, antioxidant, antihypertension, antidiabetic, and anti-inflammation. According to its various biological activities, many biologically active compounds have also been studied regarding its role on therapeutic applications such as β-glucans, proteoglycan, phenolic acids, terpenes, proteins, and sterols [29]. Extracted Pleurotus sajor-caju β-glucan has a high molecular weight and low water solubility.…”

Section: Introductionmentioning

confidence: 99%

Enhancing Activity of Pleurotus sajor-caju (Fr.) Sing β-1,3-Glucanoligosaccharide (Ps-GOS) on Proliferation, Differentiation, and Mineralization of MC3T3-E1 Cells through the Involvement of BMP-2/Runx2/MAPK/Wnt/β-Catenin Signaling Pathway

et al. 2020

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Osteoporosis is a leading world health problem that results from an imbalance between bone formation and bone resorption. β-glucans has been extensively reported to exhibit a wide range of biological activities, including antiosteoporosis both in vitro and in vivo. However, the molecular mechanisms responsible for β-glucan-mediated bone formation in osteoblasts have not yet been investigated. The oyster mushroom Pleurotus sajor-caju produces abundant amounts of an insoluble β-glucan, which is rendered soluble by enzymatic degradation using Hevea glucanase to generate low-molecular-weight glucanoligosaccharide (Ps-GOS). This study aimed to investigate the osteogenic enhancing activity and underlining molecular mechanism of Ps-GOS on osteoblastogenesis of pre-osteoblastic MC3T3-E1 cells. In this study, it was demonstrated for the first time that low concentrations of Ps-GOS could promote cell proliferation and division after 48 h of treatment. In addition, Ps-GOS upregulated the mRNA and protein expression level of bone morphogenetic protein-2 (BMP-2) and runt-related transcription factor-2 (Runx2), which are both involved in BMP signaling pathway, accompanied by increased alkaline phosphatase (ALP) activity and mineralization. Ps-GOS also upregulated the expression of osteogenesis related genes including ALP, collagen type 1 (COL1), and osteocalcin (OCN). Moreover, our novel findings suggest that Ps-GOS may exert its effects through the mitogen-activated protein kinase (MAPK) and wingless-type MMTV integration site (Wnt)/β-catenin signaling pathways.

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Multifunctions of Pleurotus sajor-caju (Fr.) Singer: A highly nutritious food and a source for bioactive compounds

Cited by 30 publications

References 34 publications

Homology models of mouse and rat estrogen receptor-α ligand-binding domain created by in silico mutagenesis of a human template: Molecular docking with 17β-estradiol, diethylstilbestrol, and paraben analogs

Homology models of mouse and rat estrogen receptor-α ligand-binding domain created by in silico mutagenesis of a human template: Molecular docking with 17β-estradiol, diethylstilbestrol, and paraben analogs

A Review on Antistaphylococcal Secondary Metabolites from Basidiomycetes

Enhancing Activity of Pleurotus sajor-caju (Fr.) Sing β-1,3-Glucanoligosaccharide (Ps-GOS) on Proliferation, Differentiation, and Mineralization of MC3T3-E1 Cells through the Involvement of BMP-2/Runx2/MAPK/Wnt/β-Catenin Signaling Pathway

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