2020
DOI: 10.1039/d0sc01589j
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Multifunctional streptavidin–biotin conjugates with precise stoichiometries

Abstract: Multifunctional streptavidin-biotin conjugates with defined stoichiometry and number of open binding pockets provide molecularly precise alternatives to the statistical mixture of products that typically forms.

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Cited by 13 publications
(13 citation statements)
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“…Tetravalent biotin–streptavidin ( STV ) binding provides a modular high-affinity strategy for protein bioconjugation. A wide range of molecular and macromolecular systems can be biotinylated, making this a highly versatile platform for bioconjugation. , We report here the use of biotin–streptavidin assemblies to provide cytosolic delivery of proteins into cells. In this system, biotinylated oligo­(glutamate) ( b-E20 ) and biotinylated proteins were first bound to STV (Figure ).…”
Section: Introductionmentioning
confidence: 99%
“…Tetravalent biotin–streptavidin ( STV ) binding provides a modular high-affinity strategy for protein bioconjugation. A wide range of molecular and macromolecular systems can be biotinylated, making this a highly versatile platform for bioconjugation. , We report here the use of biotin–streptavidin assemblies to provide cytosolic delivery of proteins into cells. In this system, biotinylated oligo­(glutamate) ( b-E20 ) and biotinylated proteins were first bound to STV (Figure ).…”
Section: Introductionmentioning
confidence: 99%
“…This is in contrast to the successful immobilization of streptavidin as demonstrated earlier, but not surprising considering the relatively poor binding of the mannose-ConA couple as compared to the biotin−streptavidin couple (K d = 2.89 × 10 −6 M and 1 × 10 −14 M, respectively). 50,51 Although from the fluorescence microscopy analysis, it was evident that, for the surface obtained using mannose modification of the G1-alkene, a slightly higher amount of ConA binding took place and it did not appear to be effective. To understand if the crowding of functional groups leads to this lack of enhanced protein binding, we decided to dilute the mannose clusters by adding AIBN during the attachment of the alkene units.…”
Section: ■ Results and Discussionmentioning
confidence: 99%
“…Next, the synthesized Fe-N x SANs were treated with N-(3-dimethylamino propyl)-N′-ethylcarbodiimide hydrochloride (EDC) and N-hydroxysuccinimide (NHS), then modified with SA to bind biotinylated A β 1-40 antibody, which is proved by the Fourier transform infrared spectra successfully (Figure S1 ) [ 32 ]. Thereinto, the biotin can react with SA-conjugated labels, forming the strongest known noncovalent interaction between a protein and a ligand [ 33 ]. Notably, the interaction is rapid and maintains being robust in extreme conditions of pH and temperature.…”
Section: Resultsmentioning
confidence: 99%