Multifunctional RNA Processing Protein SRm160 Induces Apoptosis and Regulates Eye and Genital Development in <i>Drosophila</i>

Fan, Yujie; Gittis, Aryn H.; Juge, François; Qiu, Chen; Xu, Yan; Rabinow, Léonard

doi:10.1534/genetics.114.164434

Cited by 11 publications

(12 citation statements)

References 82 publications

(112 reference statements)

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“…We found that pleiotropic alterations of SRm160 levels lead to arrested development and lethality during the larval stage. This correlates with previous data showing that SRm160 levels are recovered during larval stages (Fan et al 2014), probably reflecting a second wave of expression that is essential for development. In addition, during adult development, SRm160 enhances female-to-male somatic sex transformations and also regulates apoptosis in the adult eye (Fan et al 2014).…”

Section: Srm160 Functionssupporting

confidence: 92%

“…This correlates with previous data showing that SRm160 levels are recovered during larval stages (Fan et al 2014), probably reflecting a second wave of expression that is essential for development. In addition, during adult development, SRm160 enhances female-to-male somatic sex transformations and also regulates apoptosis in the adult eye (Fan et al 2014). Thus, our results showing that SRm160 is an adult-specific regulator of the circadian clock represent, to our knowledge, the first well-defined example of a specific biological process controlled by SRm160 beyond embryonic development and metamorphosis.…”

Section: Srm160 Functionssupporting

confidence: 92%

“…However, information regarding the breadth of SRm160 activity on the fly transcriptome in vivo, as well as at which level this gene exerts its effect, is clearly missing. For this highthroughput approach, we employed the previously characterized SRm160 18603 null mutant allele (Fan et al 2014, Figure S1 in File S4). In this way, we were able to comprehensively assess the global effect of SRm160 loss-of-function in an animal that reaches postembryonic development.…”

Section: Lack Of Srm160 Affects the Expression And Splicing Of A Restmentioning

confidence: 99%

“…However, little is known about their role as regulators of specific biological processes. During early embryonic development in the fly, SRm160 is distributed ubiquitously and at high levels, showing early zygotic gene transcription by 2-3 hr after fertilization (Fan et al 2014). After 10 hr of development, SRm160 mRNAs are enriched in the central nervous system (CNS) and completely restricted to it by 16 hr after fertilization (Fan et al 2014).…”

Section: Srm160 Functionsmentioning

confidence: 99%

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Rhythmic Behavior Is Controlled by the SRm160 Splicing Factor inDrosophila melanogaster

et al. 2017

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Circadian clocks organize the metabolism, physiology, and behavior of organisms throughout the day-night cycle by controlling daily rhythms in gene expression at the transcriptional and post-transcriptional levels. While many transcription factors underlying circadian oscillations are known, the splicing factors that modulate these rhythms remain largely unexplored. A genome-wide assessment of the alterations of gene expression in a null mutant of the alternative splicing regulator SR-related matrix protein of 160 kDa (SRm160) revealed the extent to which alternative splicing impacts on behavior-related genes. We show that affects gene expression in pacemaker neurons of the brain to ensure proper oscillations of the molecular clock. A reduced level of SRm160 in adult pacemaker neurons impairs circadian rhythms in locomotor behavior, and this phenotype is caused, at least in part, by a marked reduction in () levels. Moreover, rhythmic accumulation of the neuropeptide PIGMENT DISPERSING FACTOR in the dorsal projections of these neurons is abolished after SRm160 depletion. The lack of rhythmicity in SRm160-downregulated flies is reversed by a fully spliced construct, but not by an extra copy of the endogenous locus, showing that positively regulates levels in a splicing-dependent manner. Our findings highlight the significant effect of alternative splicing on the nervous system and particularly on brain function in an model.

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Section: Srm160 Functionssupporting

confidence: 92%

Section: Srm160 Functionssupporting

confidence: 92%

Section: Lack Of Srm160 Affects the Expression And Splicing Of A Restmentioning

confidence: 99%

Section: Srm160 Functionsmentioning

confidence: 99%

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Rhythmic Behavior Is Controlled by the SRm160 Splicing Factor inDrosophila melanogaster

et al. 2017

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“…Crosses were kept at 29 °C and the hypandrium phenotype was examined in 10-50 F1 males (Table S13). Using the genitalia GAL4 drivers esg-GAL4 NP5130 [51] and NP6333 [52] to express Abd-B.RNAi 51167 , we obtained 20 males out of 100 with developed hypandrium, among which two aberrant hypandrial bristle phenotypes were found, either bristle size reduction or bristle loss ( Fig. S11.…”

Section: Abd-b Rnai and Clonal Analysismentioning

confidence: 99%

Correlated Evolution of two Sensory Organs via a Single Cis-Regulatory Nucleotide Change

Nagy

Nuez

Savisaar

et al. 2018

Preprint

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One Sentence Summary: We identify one nucleotide substitution in a gene regulatory region contributing to evolutionary change of two distinct organs. SUMMARYDiverse traits often covary between species. The possibility that a single mutation could contribute to the evolution of several characters between species is rarely investigated as relatively few cases are dissected at the nucleotide level. Drosophila santomea has evolved additional sex comb sensory teeth on its legs and has lost two sensory bristles on its genitalia. We found that a single nucleotide substitution in an enhancer of the scute gene contributes to both changes. The mutation alters a binding site for the Hox protein Abdominal-B in the developing genitalia, leading to bristle loss, and for another factor in the developing leg, leading to bristle gain. Our study shows that morphological evolution between species can occur through a single nucleotide change affecting several sexually dimorphic traits. was not peer-reviewed) is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity.The copyright holder for this preprint (which . http://dx.doi.org/10.1101/313197 doi: bioRxiv preprint first posted online May. 2, 2018; RESULTS AND DISCUSSION "Variability is governed by many unknown laws, of which correlated growth is probably the most important."[1]Correlated evolution of traits is widespread among taxa [1,2] and can be due to pleiotropy, where a single locus causally affects several traits [3]. Pleiotropy imposes large constrains on the paths of evolution [4,5], making it crucial to assess the extent of pleiotropy to understand the evolutionary process. Empirical studies suggest that many loci influence multiple traits [3,6,7] and current data cannot reject the idea that all genetic elements have pleiotropic roles [3,8,9]. Several pleiotropic substitutions have been associated with natural variation [10][11][12]: all are coding changes and all underlie intraspecific changes (www.gephebase.org). Nevertheless it remains unclear whether pleiotropic mutations contribute also to interspecific evolution, as experimental evidence suggests that the mutations responsible for interspecies evolution may be less pleiotropic than the mutations underlying intraspecific variation [13].Here we focused on male sexual bristle evolution between Drosophila yakuba and Drosophila santomea, which diverged approximately 0.5-1 million years ago [14] and can produce fertile F1 females in the laboratory [15], facilitating genetic mapping. We found that hypandrial bristles -two prominent mecanosensory bristles located on the ventral part of male genitalia in all D. melanogaster subgroup species -are missing in D. santomea males (Fig. 1). Examination of many inbred stocks and 10 closely related species revealed that the absence of hypandrial bristles is a derived D. santomea-specific trait (Fig. 1, Tables S1-S2). No other genital bristle type was noticeably variable in number between D. yakuba and D. santomea (Fig. S1).We performed whole-ge...

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Cleaved PGAM5 dephosphorylates nuclear serine/arginine-rich proteins during mitophagy

Baba

Tanimura

Yamaguchi

et al. 2021

Biochimica et Biophysica Acta (BBA) - Molecular Cell Research

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Multifunctional RNA Processing Protein SRm160 Induces Apoptosis and Regulates Eye and Genital Development in Drosophila

Cited by 11 publications

References 82 publications

Rhythmic Behavior Is Controlled by the SRm160 Splicing Factor inDrosophila melanogaster

Rhythmic Behavior Is Controlled by the SRm160 Splicing Factor inDrosophila melanogaster

Correlated Evolution of two Sensory Organs via a Single Cis-Regulatory Nucleotide Change

Cleaved PGAM5 dephosphorylates nuclear serine/arginine-rich proteins during mitophagy

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