Multifunctional Regeneration Silicon‐Loaded Chitosan Hydrogels for MRSA‐Infected Diabetic Wound Healing

Abstract

Repeated microbial infection, excess reactive oxygen species (ROS) accumulation, cell dysfunction, and impaired angiogenesis under hyperglycemia severely inhibit diabetic wound healing. Therefore, developing multifunctional wound dressings accommodating the complex microenvironment of diabetic wounds is of great significance. Here, a multifunctional hydrogel (Regesi‐CS) is prepared by loading regeneration silicon (Regesi) in the non‐crosslinked chitosan (CS) solution, followed with freeze‐drying and hydration. As expected, the blank non‐crosslinked CS hydrogel (1%) shows great antibacterial activity against E. coli, S. aureus, and MRSA, improves fibroblasts migration, and scavenges intracellular ROS. Interestingly, after loading 1% Regesi, the Regesi‐CS (1%‐1%) hydrogel shows greater antibacterial activity, significantly promotes fibroblasts proliferation and migration, scavenges much more ROS, and substantially protects fibroblasts under oxidative stress, yet Regesi alone has no or even negative effects. In the MRSA‐infected diabetic wound model, Regesi‐CS (1%‐1%) hydrogel effectively promotes wound healing via eliminating bacterial infection, enhancing granulation tissue formation, promoting collagen deposition, and improving angiogenesis. In conclusion, Regesi‐CS hydrogel may be a potential wound dressing for effective treatment and management of chronic diabetic wounds.This article is protected by copyright. All rights reserved

Composite Aerogel Scaffolds Containing Flexible Silica Nanofiber and Tricalcium Phosphate Enable Skin Regeneration

“…The calcium ions (Ca 2+ ) and protein kinase C-alpha (PKC-α) may interact with the phospholipase C to promote cell differentiation; the calcium ions (Ca 2+ ) could also bind to calmodulin to activate actin and myosin . On the other hand, silicon ions (Si 4+ ) may increase the expression of VEGF and α-smooth muscle actin (α-SMA), thereby facilitating vascularization …”

“…42 On the other hand, silicon ions (Si 4+ ) may increase the expression of VEGF and α-smooth muscle actin (α-SMA), thereby facilitating vascularization. 43 To further analyze the wound regeneration microenvironment, H&E staining and MT staining of the excised wound tissues were performed at days 7 and 14. By day 7, all groups exhibited nonhealed full thickness wounds alongside an accumulation of inflammatory cells in the wound center as well as margins (Figure 10a).…”

Application of In Situ Mucoadhesive Hydrogel with Anti-Inflammatory and Pro-Repairing Dual Properties for the Treatment of Chemotherapy-Induced Oral Mucositis

“…Therefore, careful control of degradation-dependent hydrogel dynamics is crucial to achieving the desired effects on cells . Improved degradation properties can provide stable barrier protection to the wound, enhancing its potential application as a soft tissue repair material . Simultaneously, an appropriate degradation rate enables sustainable release and accumulation of loaded material at the desired location for therapeutic efficacy …”

Nanozymes for the Therapeutic Treatment of Diabetic Foot Ulcers

“…In summary, the therapeutic mechanism of nanozymes for diabetic wounds is mainly based on the exertion of enzyme-like activities, combined with the efficient functions of other beneficial components (e.g., Ag ions for antimicrobials, Cu ions to promote the proliferation of epithelial cells, chitosan for bacterial inhibition, curcumin for anti-inflammation and immunomodulation, and so on), which work together to regulate the microenvironment of wounds. Among them, the enzyme-like activities are primarily POD-like, OXD-like, CAT-like, and SOD-like, which can be exploited for scavenging hydrogen peroxide and superoxide anions and generating highly toxic hydroxyl radicals through the effective regulation of ROS species in the wound microenvironment.…”