Multifunctional nanoparticle platforms for in vivo MRI enhancement and photodynamic therapy of a rat brain cancer

Kopelman, Raoul; Koo, Yong Eun Lee; Philbert, Martin A.; Moffat, Bradford A.; Reddy, G. Ramachandra; McConville, Patrick; Hall, Daniel E.; Chenevert, Thomas L.; Bhojani, Mahaveer S.; Buck, Sarah M.; Rehemtulla, Alnawaz; Ross, Brian D.

doi:10.1016/j.jmmm.2005.02.061

Cited by 173 publications

(119 citation statements)

References 9 publications

Supporting

Mentioning

116

Contrasting

Order By: Relevance

“…The photodynamic mechanism of free photosensitizers in general is fundamentally different from the work presented in this paper and the previous reports on our NPs [17][18][19][20][21]. As illustrated by the minimal enzymatic reduction of the encapsulated MB discussed above, the polymeric matrix of the NPs should be capable of protecting the embedded photosensitizers from direct interaction with biological macromolecules in vivo.…”

Section: In Vitro Pdt Studies On Tumor Cells Treated With Mb-containicontrasting

confidence: 68%

“…As illustrated in the in vitro studies above, encapsulated MB permits not only sufficient light-stimulated generation of 1 O 2 , but also adequate diffusion out of the matrix to cause damage to the tumor cells. Furthermore, based on recent successful in vivo work with the similar polyacrylamide-based NPs, using the less efficient photofrin [17][18][19][20], rather than MB, as the PDT agent, we expect the MB-containing polyacrylamide NPs to be equally or more efficient in in vivo targeted treatment of glioma. Fluorescence emission at 680 nm vs time (after subtraction of photobleaching for both curves): (A) 3 mg/mL MB-containing NPs and (B) 1 μg/mL MB, respectively, when mixed in PBS (pH 7.2) with 0.45 μmol NADH and 0.05 mg diaphorase.…”

Section: In Vitro Pdt Studies On Tumor Cells Treated With Mb-containimentioning

confidence: 99%

“…In recent years, with the advancement of nanotechnology, engineered nanoparticles have received attention as a possible means of encapsulating and delivering PDT agents. Research in our laboratories [17][18][19][20][21][22] and elsewhere [23,24] has demonstrated the utility of nano-structured materials, which employ biocompatible nanoparticle matrices encapsulated with efficient photo-active molecules, for PDT. Our concept of a nanoparticle platform (NP) was initiated by the design of Photonic Explorers for Biomedical use by Biologically Localized Embedding, termed PEBBLEs.…”

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Encapsulation of methylene blue in polyacrylamide nanoparticle platforms protects its photodynamic effectiveness

Tang

Park

et al. 2008

Biochemical and Biophysical Research Communications

Self Cite

107

View full text Add to dashboard Cite

The ability to prevent methylene blue (MB), a photosensitizer, from being reduced by plasma reductases will greatly improve its efficacy in photodynamic therapy (PDT) applications. We have developed a delivery approach for PDT by encapsulating MB using nanoparticle platforms (NPs). The 30-nm polyacrylamide-based NPs provide protection for the embedded MB against reduction by diaphorase enzymes. Furthermore, our data shows the matrix-protected MB efficiently induces photodynamic damage to tumor cells. The unprecedented results demonstrate the significant in vitro photodynamic effectiveness of MB when encapsulated within NPs, which promises to open new opportunities for MB in its in vivo and clinical studies.

show abstract

Section: In Vitro Pdt Studies On Tumor Cells Treated With Mb-containicontrasting

confidence: 68%

Section: In Vitro Pdt Studies On Tumor Cells Treated With Mb-containimentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Encapsulation of methylene blue in polyacrylamide nanoparticle platforms protects its photodynamic effectiveness

Tang

Park

et al. 2008

Biochemical and Biophysical Research Communications

Self Cite

107

View full text Add to dashboard Cite

show abstract

“…MRI is a non-invasive imaging modality and has several advantages over other imaging modalities since it provides three-dimensional anatomic images with high spatial resolution. The combination of MRI with photodynamic therapy provides image-guidance for laser irradiation (14,15), and non-invasive assessment of the therapeutic efficacy of PDT (16). Previously, we have shown that contrast-enhanced MRI-guided photodynamic therapy using a bifunctional polymer conjugate containing an MRI contrast agent and a photosensitizer is effective for tumor imaging and cancer treatment (17).…”

Section: Introductionmentioning

confidence: 99%

Contrast-Enhanced MRI-Guided Photodynamic Cancer Therapy with a Pegylated Bifunctional Polymer Conjugate

et al. 2008

View full text Add to dashboard Cite

Purpose-To study contrast-enhanced MRI guided photodynamic therapy with a pegylated bifunctional polymer conjugate containing an MRI contrast agent and a photosensitizer for minimally invasive image-guided cancer treatment.Methods-Pegylated and non-pegylated poly-(L-glutamic acid) conjugates containing mesochlorin e 6 , a photosensitizer, and Gd(III)-DO3A, an MRI contrast agent, were synthesized. The effect of pegylation on the biodistribution and tumor targeting was non-invasively visualized in mice bearing MDA-MB-231 tumor xenografts with MRI. MRI-guided photodynamic therapy was carried out in the tumor bearing mice. Tumor response to photodynamic therapy was evaluated by dynamic contrast enhanced MRI and histological analysis.Results-The pegylated conjugate had longer blood circulation, lower liver uptake and higher tumor accumulation than the non-pegylated conjugate as shown by MRI. Site-directed laser irradiation of tumors resulted in higher therapeutic efficacy for the pegylated conjugate than the nonpegylated conjugate. Moreover, animals treated with photodynamic therapy showed reduced vascular permeability on DCE-MRI and decreased microvessel density in histological analysis.Conclusions-Pegylation of the polymer bifunctional conjugates reduced non-specific liver uptake and increased tumor uptake, resulting in significant tumor contrast enhancement and high therapeutic efficacy. The pegylated poly(L-glutamic acid) bifunctional conjugate is promising for contrast enhanced MRI guided photodynamic therapy in cancer treatment.

show abstract

“…The iron oxide cores provide MRI signal contrast and magneto-mobility. Multifunctional nanoparticles were constructed by simultaneously conjugating siRNA, near infrared fluorophores (Cy5.5) Chemical conjugation [155,156,160,166] Encapsulation (liposomes) [154] Hydrophobic interaction (micelles and nanoparticles coated with amphiphilic polymers) [147150] Electrostatic interaction (positively charged liposomes, polymers and mesoporous silica) [144,159] Degradability (pH sensitive, enzyme cleavable, and reducible bonds) [160,166] Heat generation (infrared laser, alternating magnetic field and ultrasound) [145,165] Magneto-mobility (magnetic nanoparticles) [144,167,168] Photosensitizer [162] and cell membrane translocation peptides to dextran coated iron oxide nanoparticles [155]. Tumor accumulation of the nanoparticles was confirmed with both MRI T 2 imaging and fluorescence imaging and was in good agreement with the knockdown effects of siRNAs.…”

Section: Nanoparticles For Combined Imaging and Therapymentioning

confidence: 99%

Engineering imaging probes and molecular machines for nanomedicine

Tong

Cradick

et al. 2012

Sci. China Life Sci.

View full text Add to dashboard Cite

Nanomedicine is an emerging field that integrates nanotechnology, biomolecular engineering, life sciences and medicine; it is expected to produce major breakthroughs in medical diagnostics and therapeutics. Due to the size-compatibility of nano-scale structures and devices with proteins and nucleic acids, the design, synthesis and application of nanoprobes, nanocarriers and nanomachines provide unprecedented opportunities for achieving a better control of biological processes, and drastic improvements in disease detection, therapy, and prevention. Recent advances in nanomedicine include the development of functional nanoparticle based molecular imaging probes, nano-structured materials as drug/gene carriers for in vivo delivery, and engineered molecular machines for treating single-gene disorders. This review focuses on the development of molecular imaging probes and engineered nucleases for nanomedicine, including quantum dot bioconjugates, quantum dot-fluorescent protein FRET probes, molecular beacons, magnetic and gold nanoparticle based imaging contrast agents, and the design and validation of zinc finger nucleases (ZFNs) and TAL effector nucleases (TALENs) for gene targeting. The challenges in translating nanomedicine approaches to clinical applications are discussed. Nanomedicine is an emerging field that integrates nanotechnology, biomolecular engineering, biology, and medicine [1]. It focuses on the development of engineered nano-scale (1100 nm) materials, structures and devices for better diagnostics and highly specific medical intervention in curing disease or repairing damaged tissues. As a basis for nanomedicine, nanotechnology is the science, engineering, and technology related to the understanding and control of matter at the nano-scale, and the development of materials, devices, and systems that have novel properties and functions due to their nano-scale dimensions or components. Nanotechnology also provides new abilities to measure, control and manipulate matter (including soft matter) at the nano-scale what was unthinkable with conventional tools. Owing to the size-compatibility of nano-scale structures with proteins and nucleic acids in living cells, nanomedicine approaches have the potential to provide unprecedented opportunities for achieving a better control of biological processes, and drastic improvements in disease detection, therapy, and prevention, thus revolutionizing medicine. Over the last ten years or so, significant efforts have been made in the US, China, Europe and elsewhere to develop nanomedicine. For example, the US National Institutes of Health has developed a nanomedicine centers network, and invested a significant amount of research funding to nanomedicine development. Just in FY 2009 (Oct. 1, 2008Sept. 30, 2009, the total NIH funding in nanotechnology/ nanoscience projects was more than 410 million US dollars. SPECIAL TOPIC

show abstract

Multifunctional nanoparticle platforms for in vivo MRI enhancement and photodynamic therapy of a rat brain cancer

Cited by 173 publications

References 9 publications

Encapsulation of methylene blue in polyacrylamide nanoparticle platforms protects its photodynamic effectiveness

Encapsulation of methylene blue in polyacrylamide nanoparticle platforms protects its photodynamic effectiveness

Contrast-Enhanced MRI-Guided Photodynamic Cancer Therapy with a Pegylated Bifunctional Polymer Conjugate

Engineering imaging probes and molecular machines for nanomedicine

Contact Info

Product

Resources

About