Multifunctional, multicompartment polyorganosiloxane magnetic nanoparticles for biomedical applications

Utech, Stefanie; Scherer, Christian; Maskos, Michael

doi:10.1016/j.jmmm.2009.02.043

Cited by 19 publications

(15 citation statements)

References 13 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Multicompartments have been developed in a variety of setups by using different combinations of lipid membranes, (in)organic capsules, amphiphilic polymer membranes, and multilayer polyelectrolyte membranes . For example, simple responsive cellular mimics were proposed using capsosome‐based compartments loaded with stimuli‐responsive adamantyl‐modified polymersomes, capsules containing pH‐dependent liposomes, lipid giant vesicles loaded with UV‐responsive liposomes, and microscale polymersomes encapsulated with temperature‐dependent liposomes to trigger specific chemical reactions …”

Section: Introductionmentioning

confidence: 99%

Mimicking Cellular Signaling Pathways within Synthetic Multicompartment Vesicles with Triggered Enzyme Activity and Induced Ion Channel Recruitment

Thamboo

Najer

Belluati

et al. 2019

Adv Funct Materials

View full text Add to dashboard Cite

Subcellular compartmentalization of cells, a defining characteristic of eukaryotes, is fundamental for the fine tuning of internal processes and the responding to external stimuli. Reproducing and controlling such compartmentalized hierarchical organization, responsiveness, and communication is important toward understanding biological systems and applying them to smart materials. Herein, a cellular signal transduction strategy (triggered release from subcompartments) is leveraged to develop responsive, purely artificial, polymeric multicompartment assemblies. Incorporation of responsive nanoparticles-loaded with enzymatic substrate or ion channels-as subcompartments inside micrometersized polymeric vesicles (polymersomes) allowed to conduct bioinspired signaling cascades. Response of these subcompartments to an externally added stimulus is achieved and studied by using confocal laser scanning micro scopy (CLSM) coupled with in situ fluorescence correlation spectroscopy (FCS). Signal triggered activity of an enzymatic reaction is demonstrated in multicompartments through recombination of compartmentalized substrate and enzyme. In parallel, a two-step signaling cascade is achieved by triggering the recruitment of ion channels from inner subcompartments to the vesicles' membrane, inducing ion permeability, mimicking endosome-mediated insertion of internally stored channels. This design shows remarkable versatility, robustness, and controllability, demonstrating that multicompartment polymer-based assemblies offer an ideal scaffold for the development of complex cell-inspired responsive systems for applications in biosensing, catalysis, and medicine.

show abstract

Section: Introductionmentioning

confidence: 99%

Mimicking Cellular Signaling Pathways within Synthetic Multicompartment Vesicles with Triggered Enzyme Activity and Induced Ion Channel Recruitment

Thamboo

Najer

Belluati

et al. 2019

Adv Funct Materials

View full text Add to dashboard Cite

show abstract

“…Such hybrid particles, consisting of inorganic and polymeric materials have been used for example in drug delivery (magnetite particles), [27,175] cancer treatment (cis-platin), [25] cell labeling (lanthanide complexes or quantum dots) [165,176] and lithographic applications. [121] Despite the variety of applications, the determination of the actual metal content in these hybrid nanoparticles has rarely been reported.…”

Section: Introductionmentioning

confidence: 99%

Surface Patterning with Colloidal Monolayers

Vogel

2012

Springer Theses

View full text Add to dashboard Cite

This thesis focuses on the controlled assembly of monodisperse polymer colloids into ordered two-dimensional arrangements. These assemblies, commonly referred to as colloidal monolayers, are subsequently used as masks for the generation of arrays of complex metal nanostructures on solid substrates.The motivation of the research presented here is twofold. First, monolayer crystallization methods were developed to simplify the assembly of colloids and to produce more complex arrangements of colloids in a precise way. Second, various approaches to colloidal lithography are designed with the aim to include novel features or functions to arrays of metal nanostructures.The air/water interface was exploited for the crystallization of colloidal monolayer architectures as it combines a two-dimensional confinement with a high lateral mobility of the colloids that is beneficial for the creation of high long range order. A direct assembly of colloids is presented that provides a cheap, fast and conceptually simple methodology for the preparation of ordered colloidal monolayers. The produced two-dimensional crystals can be transformed into nonclose-packed architectures by a plasma-induced size reduction step, thus providing valuable masks for more sophisticated lithographic processes. Finally, the controlled co-assembly of binary colloidal crystals with defined stoichiometries on a Langmuir trough is introduced and characterized with respect to accessible configurations and size ratios.Several approaches to lithography are presented that aim at introducing different features to colloidal lithography. First, using metal-complex containing latex particles, the synthesis of which is described as well, symmetric arrays of metal nanoparticles can be created by controlled combustion of the organic material of the colloids. The process does not feature an inherent limit in nanoparticle size and is able to produce complex materials as will be demonstrated for FePt alloy particles. Precise control over both size and spacing of the particle array is presented.Second, two lithographic processes are introduced to create sophisticated nanoparticle dimer units consisting of two crescent shaped nanostructures in close proximity; essentially by using a single colloid as mask to generate two structures simultaneously. Strong coupling processes of the parental plasmon resonances of the two objects are observed that are accompanied by high near-field enhancements. A plasmon hybridization model is elaborated to explain all polarization dependent shifts of the resonance positions. Last, a technique to produce laterally patterned, ultra-flat substrates without surface topographies by embedding gold nanoparticles in a silicon dioxide matrix is applied to construct robust and re-usable sensing architectures and to introduce an approach for the nanoscale patterning of solid supported lipid bilayer membranes.

show abstract

“…Strikingly, all particles separated contain more than one magnetic iron oxide nanoparticle. Calculating the particle sizes that would be necessary for a magnetic separation using a cylindrical magnet (diameter 2, length 3 mm, remanescent magnetic induction B r ¼1.2 T) we found a minimum magnetic core radius of R¼19.3 nm and a magnetic volume of V¼30 098 nm 3 for a capsule with a diamagnetic shell of 14.5 nm thickness [14]. Based on the TEM image shown in Fig.…”

Section: Magnetic Separationmentioning

confidence: 96%

“…In a previous publication, we reported the incorporation of hydrophobically stabilized magnetic iron oxide nanoparticles synthesized via thermal decomposition of iron pentacarbonyl using oleic acid as capping agent into the polyorganosiloxane system [14]. To provide compatibility between the hydrophobic inorganic particles and the siloxane system the magnetic particles had to be dispersed in octadecyltrimethoxysilane (OD-T) before incorporation.…”

Section: Magnetic Iron Oxide Nanoparticlesmentioning

confidence: 99%

Magnetic polyorganosiloxane core–shell nanoparticles: Synthesis, characterization and magnetic fractionation

Utech

Scherer

Krohne

et al. 2010

Journal of Magnetism and Magnetic Materials

Self Cite

View full text Add to dashboard Cite

Multifunctional, multicompartment polyorganosiloxane magnetic nanoparticles for biomedical applications

Cited by 19 publications

References 13 publications

Mimicking Cellular Signaling Pathways within Synthetic Multicompartment Vesicles with Triggered Enzyme Activity and Induced Ion Channel Recruitment

Mimicking Cellular Signaling Pathways within Synthetic Multicompartment Vesicles with Triggered Enzyme Activity and Induced Ion Channel Recruitment

Surface Patterning with Colloidal Monolayers

Magnetic polyorganosiloxane core–shell nanoparticles: Synthesis, characterization and magnetic fractionation

Contact Info

Product

Resources

About