Multifunctional Interleukin-24 Resolves Neuroretina Autoimmunity via Diverse Mechanisms

Zhang, Xuan; Hu, Cuiping; Zhong, Yajie; Qiao, Dijie; Chi, Wei; Shen, Huangxuan; Chong, Wai Po

doi:10.3390/ijms231911988

Cited by 5 publications

(1 citation statement)

References 37 publications

(59 reference statements)

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“…[ 13 ] IL-24, along with other members of the IL-10 family, such as IL-19 and IL-20, promotes the growth of keratinocytes in 2D cell cultures. [ 14 ] Like IL-19 and IL-20, IL-24 causes an increase in the thickness of the outer layer of the skin and the growth of skin cells in a human epidermis culture system. [ 15 ] This effect is directly proportional to the amount of IL-24 utilized.…”

Section: Introductionmentioning

confidence: 99%

Serum level of interleukin-24 and its polymorphism in eczematic Iraqi patients

Mahmood,

Al-Bassam

2024

Medicine

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Eczema is a common skin disease associated with inflammation. Interleukin (IL)-24 is crucial in the pathogenesis of inflammatory diseases like eczema. The study objective was the assessment of IL-24 serum levels and its gene polymorphisms in eczematic Iraqi patients. This retrospective case-control study involved 145 participants, divided into 82 patients with eczema and 63 healthy controls. An enzyme-linked immunosorbent assay measured serum IL-24, while polymerase chain reaction and Sanger DNA sequencing were used for genotype analysis. Serum IL-24 level was significantly higher (P value < .001) in patients compared to controls (41.6 [interquartile range (IQR): 28.9–53.6] vs 9.8 [IQR: 0.8–19.6] pg/mL, respectively). DNA sequence illustrated 2 SNPs with polymorphic frequencies (rs1150256 G/A and rs3093425 del/ins). The first SNP (rs1150256 G/A) showed 3 genotypes (GG, AA, and G/A), while the second SNP (rs3093425) showed 3 genotypes (-/G del/Ins, G Ins/Ins, and - del/del). The subsequent investigation revealed the presence of the following findings within the DNA sequence of the PCR amplified region (329bp). In the control group, all participants had GG/G (wild type) genotype/allele for the rs1150256 SNP, while in eczematic patients, 24.4% GG, 50% GA, and 25.6% AA. For the second SNP genotype (rs3093425 del/ins), the genotype frequencies in patients vs control were (24.4% vs 84.1%, 50.0% vs 11.1%, and 25.6% vs 4.8; Del/Del, Del/Ins, and Ins/Ins, respectively). The presence of Ins compared to Del increased the risk of eczema by 8.91 (4.66–17.03); OR (95% CI). In conclusion, IL-24 is a good predictor of eczema and A-allele carrier for rs1150256 SNP, and insertion-allele carrier for rs3093425 SNP is associated with elevated serum IL-24 and higher risk of eczema.

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Section: Introductionmentioning

confidence: 99%

Serum level of interleukin-24 and its polymorphism in eczematic Iraqi patients

Mahmood,

Al-Bassam

2024

Medicine

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Novel insight into MDA-7/IL-24: A potent therapeutic target for autoimmune and inflammatory diseases

Feng,

Cen,

Zou

et al. 2024

Clinical Immunology

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Protective role of IL-17-producing γδ T cells in a laser-induced choroidal neovascularization mouse model

Chang,

Hsing,

Chiu

et al. 2023

J Neuroinflammation

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Background Vision loss in patients with wet/exudative age-related macular degeneration (AMD) is associated with choroidal neovascularization (CNV), and AMD is the leading cause of irreversible vision impairment in older adults. Interleukin-17A (IL-17A) is a component of the microenvironment associated with some autoimmune diseases. Previous studies have indicated that wet AMD patients have elevated serum IL-17A levels. However, the effect of IL-17A on AMD progression needs to be better understood. We aimed to investigate the role of IL-17A in a laser-induced CNV mouse model. Methods We established a laser-induced CNV mouse model in wild-type (WT) and IL-17A-deficient mice and then evaluated the disease severity of these mice by using fluorescence angiography. We performed enzyme-linked immunosorbent assay (ELISA) and fluorescence-activated cell sorting (FACS) to analyze the levels of IL-17A and to investigate the immune cell populations in the eyes of WT and IL-17A-deficient mice. We used ARPE-19 cells to clarify the effect of IL-17A under oxidative stress. Results In the laser-induced CNV model, the CNV lesions were larger in IL-17A-deficient mice than in WT mice. The numbers of γδ T cells, CD3+CD4+RORγt+ T cells, Treg cells, and neutrophils were decreased and the number of macrophages was increased in the eyes of IL-17A-deficient mice compared with WT mice. In WT mice, IL-17A-producing γδ T-cell numbers increased in a time-dependent manner from day 7 to 28 after laser injury. IL-6 levels increased and IL-10, IL-24, IL-17F, and GM-CSF levels decreased in the eyes of IL-17A-deficient mice after laser injury. In vitro, IL-17A inhibited apoptosis and induced the expression of the antioxidant protein HO-1 in ARPE-19 cells under oxidative stress conditions. IL-17A facilitated the repair of oxidative stress-induced barrier dysfunction in ARPE-19 cells. Conclusions Our findings provide new insight into the protective effect of IL-17A in a laser-induced CNV model and reveal a novel regulatory role of IL-17A-producing γδ T cells in the ocular microenvironment in wet AMD.

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Multifunctional Interleukin-24 Resolves Neuroretina Autoimmunity via Diverse Mechanisms

Cited by 5 publications

References 37 publications

Serum level of interleukin-24 and its polymorphism in eczematic Iraqi patients

Serum level of interleukin-24 and its polymorphism in eczematic Iraqi patients

Novel insight into MDA-7/IL-24: A potent therapeutic target for autoimmune and inflammatory diseases

Protective role of IL-17-producing γδ T cells in a laser-induced choroidal neovascularization mouse model

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