Multifunctional Gold Nanorods with Ultrahigh Stability and Tunability for In Vivo Fluorescence Imaging, SERS Detection, and Photodynamic Therapy

Zhang, Yuan; Qian, Jun; Wang, Dan; Wang, Yalun; He, Sailing

doi:10.1002/anie.201207909

Cited by 230 publications

(141 citation statements)

References 28 publications

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“…27 In this respect, fluorescent-SERS tags have been successfully used for imaging of duplex coexpressed markers on cancer cells, 28 as well as for simultaneous multimodal tumor detection and photodynamic therapy in vivo. 29 Although most of the reported strategies comprise the conjugation of a fluorophore onto the plasmonic core, 30−32 we alternatively propose the use of fluorescently labeled PS beads as carriers to attach the SERS-active Au NSs. Compared to previously reported fluorescent-SERS dual mode tags, our multifunctional nanoprobes are more robust due to the use of PS supports, 15 and the colocalization of the plasmonic nanostars on the colloidal structure allows the detection of the intracellular SERS signal.…”

Section: ■ Introductionmentioning

confidence: 99%

Gold Nanostar-Coated Polystyrene Beads as Multifunctional Nanoprobes for SERS Bioimaging

et al. 2016

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Hybrid colloidal nanocomposites comprising polystyrene beads and plasmonic gold nanostars are reported as multifunctional optical nanoprobes. Such self-assembled structures are excellent Raman enhancers for bioapplications as they feature plasmon modes in the near-infrared "first biological transparency window". In this proof of concept study, we used 4-mercaptobenzoic acid as a Raman-active molecule to optimize the density of gold nanostars on polystyrene beads, improving SERS performance and thereby allowing in vitro cell culture imaging. Interestingly, intermediate gold nanostar loadings were found to yield higher SERS response, which was confirmed by electromagnetic modeling. These engineered hybrid nanostructures notably improve the possibilities of using gold nanostars as SERS tags. Additionally, when fluorescently labeled polystyrene beads are used as colloidal carriers, the composite particles can be applied as promising tools for multimodal bioimaging.

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Section: ■ Introductionmentioning

confidence: 99%

Gold Nanostar-Coated Polystyrene Beads as Multifunctional Nanoprobes for SERS Bioimaging

et al. 2016

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“…To evaluate the similarity between pure and reconstructed multispectral images, a distance metric proposed by Nascimento, spectral angle distance (SAD), is adopted [15]. SAD is expressed as…”

Section: Similarity Metricmentioning

confidence: 99%

Compressive Sampling Multispectral Imaging and Unmixing Method for Fluorescent Imaging

Song¹,

Cai²,

Evans³

et al. 2016

PIER M

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Abstract-Multispectral imaging is an important tool for understanding composite materials in many disciplines. Spectral unmixing enables the determination of individual fluorophore distributions. Due to the dispersive nature of biomaterials the observed spectra of fluorescent dyes is unknown. Spectral unmixing can be accomplished for unknown endmember spectra using minimum volume simplex analysis (MVSA). Compressive sampling (CS) is a method to reduce the computational cost of operating on sparse data sets and can be performed efficiently using NESTA based on Nesterov's algorithm. Here we demonstrate that NESTA and MVSA can be combined with a denoising threshold to create a compressive sampling and multispectral unmixing (CSMIU) method that enables efficient bioimaging and unmixing with high levels of accuracy (spectral angle distances (SADs) < 0.05). This CSMIU method may potentially enable broadband and in vivo bioimaging modalities.

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“…Recently, F-SERS tags have been successfully used for the duplex detection of different markers co-expressed on breast cancer cells 10 and in vivo fluorescence imaging, SERS detection, and photodynamic therapy in living mouse. 15,16 The selection of fluorophores determined the fluorescent properties of F-SERS tags. Organic dyes derivatives, such as fluorescein isothiocyanate (FITC), Rhodamine B isothiocyanate (RBITC)-labeled silica precursors, 9,10,17 or polyelectrolytes 18,19 had been selected to generate fluorescence of dual mode tags via the conjugation on the SERS cores through silane chemistry or electrostatic force induced layer-by-layer deposition.…”

Section: ■ Introductionmentioning

confidence: 99%

Upconversion Fluorescence-SERS Dual-Mode Tags for Cellular and in Vivo Imaging

Xiao-Juan

Chen

Wang

et al. 2014

ACS Appl. Mater. Interfaces

110

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Fluorescent-surface enhanced Raman scattering (F-SERS) dual mode tags showed great potential for bioimaging due to the combined advantages of intuitive, fast imaging of fluorescence and multiplex capability of SERS technique. In previously reported F-SERS tags, organic fluorescent dyes or quantum dots were generally selected to generate fluorescence signal. Herein, we reported the first proof-of-concept upconversion fluorescence (UCF)-SERS dual mode tags based on near infrared (NIR) laser (980 nm) excited upconversion nanoparticles (UCNPs) for live-cell and in vivo imaging. Three components involved in this tag: NaYF 4 :Yb,Er UCNPs@SiO 2 serving as the fluorescent core of the tag; silver nanoparticles in situ grown on the surface of UCNPs@SiO 2 for generating characteristic Raman signal; and denatured BSA coating rendering the tag's stability and biocompatibility. The UCF-SERS tags integrated the NIR imaging capability of both fluorescent UCNPs and plasmonic SERS nanoprobe, which facilitated dual mode bioimaging investigation, especially for living animals. Ex vivo experiments revealed that with 980 nm and 785 nm NIR laser irradiations, the UCF and SERS signals of the tags could be detected from 3 and 7 mm deep pork tissues, respectively. Furthermore, the in vivo imaging capabilities of UCF-SERS tags were successfully demonstrated on living mice. The developed dual modality tags held great potential for medical diagnostics and therapy.

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Multifunctional Gold Nanorods with Ultrahigh Stability and Tunability for In Vivo Fluorescence Imaging, SERS Detection, and Photodynamic Therapy

Cited by 230 publications

References 28 publications

Gold Nanostar-Coated Polystyrene Beads as Multifunctional Nanoprobes for SERS Bioimaging

Gold Nanostar-Coated Polystyrene Beads as Multifunctional Nanoprobes for SERS Bioimaging

Compressive Sampling Multispectral Imaging and Unmixing Method for Fluorescent Imaging

Upconversion Fluorescence-SERS Dual-Mode Tags for Cellular and in Vivo Imaging

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