Multifunctional, fluorescent DNA-derived carbon dots for biomedical applications: bioimaging, luminescent DNA hydrogels, and dopamine detection

Pandey, Pankaj; Preeti, .; Rawat, Kamla; Prasad, Tulika; Bohidar, H. B.

doi:10.1039/c9tb01863h

Cited by 62 publications

(34 citation statements)

References 39 publications

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“…All obtained solutions were strongly fluorescent under UV-A (365 nm) irradiation and, visually, intensities and colours of fluorescent light of obtained products were notably different ( Figure 2 B). TEM observations of the solutions obtained after the HT treatment of DNA confirmed the presence of small nanoparticles with an average size of 7.8 ± 2.5 nm ( Figure 2 C), in agreement with the size of DNA biodots reported in the literature that was in the range of 5–20 nm [ 39 , 40 , 41 , 42 ]. Samples prepared from DNA and RNA contained black non-fluorescent precipitate (ca.…”

Section: Resultssupporting

confidence: 89%

“…In contrast to carbohydrate-derived CDs, only a few studies have attempted to synthesize fluorescent nanomaterials from DNA by the HT method so far [ 39 , 40 , 41 , 42 ]. Ding et al successfully prepared and applied DNA-derived CDs for simultaneous DNA delivery and visualization [ 39 ].…”

Section: Introductionmentioning

confidence: 99%

“…By comparison of CDs prepared from DNA and those from individual DNA bases and base pairs, it was noted that only cytosine bases or cytosine-containing base pairs could be converted to CDs with a bright fluorescence comparable to CDs from DNA. Pandey et al used salmon sperm DNA to prepare multifunctional CDs of 4–5 nm size for bioimaging, drug delivery by CDs hydrogel, and biosensing [ 41 ]. At present, however, to the best of our knowledge, only the four above studies are available and the process of fluorescent dots formation from DNA and factors that control fluorescent properties of CDs are still poorly understood.…”

Section: Introductionmentioning

confidence: 99%

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Fluorescent Nanoparticles Synthesized from DNA, RNA, and Nucleotides

et al. 2021

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Ubiquitous on Earth DNA and other nucleic acids are being increasingly considered as promising biomass resources. Due to their unique chemical structure, which is different from that of a more common carbohydrate biomass polymers, materials based on nucleic acids may exhibit new attractive characteristics. In this study, fluorescent nanoparticles (biodots) were prepared by a hydrothermal (HT) method from various nucleic acids (DNA, RNA, nucleotides, and nucleosides) to establish the relationship between the structure of precursors and fluorescent properties of biodots and to optimize conditions for preparation of the most fluorescent product. HT treatment of nucleic acids results in decomposition of sugar moieties and depurination/depyrimidation of nucleobases, while their consequent condensation and polymerization gives fluorescent nanoparticles. Fluorescent properties of DNA and RNA biodots are drastically different from biodots synthesized from individual nucleotides. In particular, biodots synthesized from purine-containing nucleotides or nucleosides show up to 50-fold higher fluorescence compared to analogous pyrimidine-derived biodots. The polymeric nature of a precursor disfavors formation of a bright fluorescent product. The reported effect of the structure of the nucleic acid precursor on the fluorescence properties of biodots should help designing and synthesizing brighter fluorescent nanomaterials with broader specification for bioimaging, sensing, and other applications.

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Section: Resultssupporting

confidence: 89%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Fluorescent Nanoparticles Synthesized from DNA, RNA, and Nucleotides

et al. 2021

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“…In addition, dopamine in pharmaceutical samples was successfully analyzed using this new platform. Many similar nanomaterial‐enhanced sensors were constructed and the commonly used nanomaterials include CNTs, 85‐87 graphene, 88‐90 CDs, 91,92 QDs, 93 AuNPs, 94,95 silver nanoparticles, 96,97 metal oxides, 98,99 and metal‐organic frameworks (MOFs) 3,100–102 . Fast‐scan cyclic voltammetry (FSCV) is a popular method for real‐time detection of NTs in vivo because of its fast detection speed.…”

Section: Electochemical Biosensors Based On Oxidation Of Dopaminementioning

confidence: 99%

Biosensors and sensors for dopamine detection

Liu

2020

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Dopamine is a key catecholamine neurotransmitter and it has critical roles in the function of the human central nervous system. Abnormal release of dopamine is related to neurological diseases and depression. Therefore, it is necessary to monitor dopamine levels in vivo and in real time to understand its physiological roles. In this review, we discuss dopamine detection focusing on the molecular recognition methods including enzymes, antibodies, and aptamers, as well as new advances based on nanomaterials and molecularly imprinted polymers (MIPs). A large fraction of these sensors rely on electrochemical detection to fulfill the requirement of fast, in situ, and in vivo detection with a high spatial and temporal resolution. These methods need to overcome interferences from molecules with a similar redox potential. In addition, fluorescent and colorimetric sensors based on aptamers are also quite popular, and care needs to be taken to validate specific dopamine binding. Combining aptamers or MIPs with electrochemistry promises to achieve rapid detection and increased selectivity. In this article, we pay more attention to the molecular recognition mechanism and critically review the sensor designs. In the end, some future directions are discussed.

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“…Either the cross‐linking between the DNA building blocks and nanomaterials is formed via physical interaction, or the nanomaterials are encapsulated in the DNA hydrogel networks. The nanomaterials applied for the development of hybrid NAHs cover a variety of categories, including carbon‐based nanomaterials, such as GO, [ 76 , 77 ] carbon‐nanotubes, [ 78 , 79 ] carbon dots, [ 80 , 81 , 82 ] quantum dots, [ 83 ] magnetic beads, [ 84 , 85 , 86 , 87 , 88 ] liposome, [ 57 ] gold nanoparticles (AuNPs), [ 68 , 75 , 89 , 90 , 91 ] silica nanoparticles, [ 54 , 92 ] Laponite nanoparticles, [ 93 , 94 , 95 ] and photonic crystal (PC). [ 96 ] The interaction between DNA and nanomaterials reveals their multiple functional features, further enhancing the corresponding applications.…”

Section: Functional Units and Their Assembly Strategies For Nahsmentioning

confidence: 99%

Smart and Functionalized Development of Nucleic Acid‐Based Hydrogels: Assembly Strategies, Recent Advances, and Challenges

et al. 2021

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Nucleic acid-based hydrogels that integrate intrinsic biological properties of nucleic acids and mechanical behavior of their advanced assemblies are appealing bioanalysis and biomedical studies for the development of new-generation smart biomaterials. It is inseparable from development and incorporation of novel structural and functional units. This review highlights different functional units of nucleic acids, polymers, and novel nanomaterials in the order of structures, properties, and functions, and their assembly strategies for the fabrication of nucleic acid-based hydrogels. Also, recent advances in the design of multifunctional and stimuli-responsive nucleic acid-based hydrogels in bioanalysis and biomedical science are discussed, focusing on the applications of customized hydrogels for emerging directions, including 3D cell cultivation and 3D bioprinting. Finally, the key challenge and future perspectives are outlined.

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Multifunctional, fluorescent DNA-derived carbon dots for biomedical applications: bioimaging, luminescent DNA hydrogels, and dopamine detection

Abstract: Here, we describe the synthesis of 2–3 nm, hydrophilic, blue fluorescence-emitting carbon dots (C-Dots, made using a DNA precursor) by the hydrothermal route from the gelling concentration of 2% (w/v) DNA.

Cited by 62 publications

References 39 publications

Fluorescent Nanoparticles Synthesized from DNA, RNA, and Nucleotides

Fluorescent Nanoparticles Synthesized from DNA, RNA, and Nucleotides

Biosensors and sensors for dopamine detection

Smart and Functionalized Development of Nucleic Acid‐Based Hydrogels: Assembly Strategies, Recent Advances, and Challenges

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