Multifunctional effect of epigallocatechin-3-gallate (EGCG) in downregulation of gelatinase-A (MMP-2) in human breast cancer cell line MCF-7

Sen, Triparna; Moulik, Shuvojit; Dutta, Anindita; Choudhury, Paromita Roy; Banerji, Aniruddha; Das, Shamik; Roy, Madhumita; Chatterjee, Amitava

doi:10.1016/j.lfs.2008.11.018

Cited by 86 publications

(63 citation statements)

References 51 publications

(41 reference statements)

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“…For example, Epigallocatechin gallate (EGCG) and Paclitaxel (PTX) were co-formulated within a targeted core shell PLGA-casein nanoparticle to be released in sequence to allow for NF-κB downregulation and enhanced activity of PTX [174]. Certain NF-κB-inducible genes have been shown to protect MDA-MB-231 human breast cancer cells against Paclitaxel [175], and EGCG has demonstrated down-regulation of NF-κB amongst other key regulatory proteins [176]. The nanoparticles re-sensitized PTX-resistant human breast cancer cells to PTX, afforded substantial cytotoxicity, and repressed expression of P-gp [174].…”

Section: Optimal Design Of Delivery Systems For Combination Therapymentioning

confidence: 99%

“Combo” nanomedicine: Co-delivery of multi-modal therapeutics for efficient, targeted, and safe cancer therapy

Kemp

Shim

Heo

et al. 2016

Advanced Drug Delivery Reviews

423

258

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a b s t r a c t a r t i c l e i n f oThe dynamic and versatile nature of diseases such as cancer has been a pivotal challenge for developing efficient and safe therapies. Cancer treatments using a single therapeutic agent often result in limited clinical outcomes due to tumor heterogeneity and drug resistance. Combination therapies using multiple therapeutic modalities can synergistically elevate anti-cancer activity while lowering doses of each agent, hence, reducing side effects. Co-administration of multiple therapeutic agents requires a delivery platform that can normalize pharmacokinetics and pharmacodynamics of the agents, prolong circulation, selectively accumulate, specifically bind to the target, and enable controlled release in target site. Nanomaterials, such as polymeric nanoparticles, gold nanoparticles/cages/shells, and carbon nanomaterials, have the desired properties, and they can mediate therapeutic effects different from those generated by small molecule drugs (e.g., gene therapy, photothermal therapy, photodynamic therapy, and radiotherapy). This review aims to provide an overview of developing multi-modal therapies using nanomaterials ("combo" nanomedicine) along with the rationale, up-to-date progress, further considerations, and the crucial roles of interdisciplinary approaches.

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Section: Optimal Design Of Delivery Systems For Combination Therapymentioning

confidence: 99%

“Combo” nanomedicine: Co-delivery of multi-modal therapeutics for efficient, targeted, and safe cancer therapy

Kemp

Shim

Heo

et al. 2016

Advanced Drug Delivery Reviews

423

258

View full text Add to dashboard Cite

show abstract

“…The levels of MMP-2, MMP -9, TIMP-1 and TIMP-2 gene expression were analyzed with real time RT-PCR as described previously with slight modifications [31]. RNA was extracted from Vero cells which were seeded at a density of 5 × 10 4 cells/mL and infected with DV3 (MOI of 1 ffu/cell) for 48 h at 37 °C in presence and absence of various concentrations of ZOA and 100 μM EGCG.…”

Section: Real-time Rt-pcr For Mmp-2 Mmp-9 Timp-1 and Timp-2 Mrnamentioning

confidence: 99%

Zingiber officinale Roscoe aqueous extract modulates Matrixmetalloproteinases and tissue inhibitors of Metalloproteinases expressions in Dengue virus-infected cells: implications for prevention of vascular permeability

Sharma¹,

Klinzing²,

Ramos³

2015

Trop. J. Pharm Res

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“…To assay the gelatinase activity, Gelatin Zymography was performed using a 7.5 % SDS-PAGE (sodium dodecyl sulfate polyacrylamide gel electrophoresis) co-polymerized with 0.1 % gelatin as described in earlier report [17] with only modification of 44 h incubation in reaction buffer.…”

Section: Gelatin Zymographymentioning

confidence: 99%

Extracellular Matrix Protein Laminin Induces Matrix Metalloproteinase-9 in Human Breast Cancer Cell Line MCF-7

Pal

Moulik

Dutta

et al. 2014

Cancer Microenvironment

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Studies on interaction of tumor cells with extracellular matrix (ECM) components showed increased extracellular protease activity mediated by the family of matrix metalloproteinases (MMPs). Here we studied the effect of human breast cancer cell line MCF-7-laminin (LM) interaction on MMPs and the underlying signaling pathways. Culturing of MCF-7 cells on LM coated surface upregulated MMP-9 expression as well as reduced tissue inhibitor of metalloproteinases-1 (TIMP-1) expression. LM induced MMP-9 expression is abrogated by the blockade of α 2 integrin. Inhibitor studies indicate possible involvement of phosphatidyl-inositol-3-kinase (PI3K), extracellular signal regulated kinase (ERK) and nuclear factor-kappaB (NF-κB) in LM induced signaling. LM treatment also enhanced phosphorylation of FAK (focal adhesion kinase), PI3K, ERK; nuclear translocation of ERK, pERK, NF-κB and cell migration. Our findings indicate that, binding of MCF-7 cells to LM, possibly via α 2 β 1 integrin, induces signaling involving FAK, PI3K, ERK, NF-κB followed by upregulation of MMP-9 and cell migration.

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Multifunctional effect of epigallocatechin-3-gallate (EGCG) in downregulation of gelatinase-A (MMP-2) in human breast cancer cell line MCF-7

Cited by 86 publications

References 51 publications

“Combo” nanomedicine: Co-delivery of multi-modal therapeutics for efficient, targeted, and safe cancer therapy

“Combo” nanomedicine: Co-delivery of multi-modal therapeutics for efficient, targeted, and safe cancer therapy

Zingiber officinale Roscoe aqueous extract modulates Matrixmetalloproteinases and tissue inhibitors of Metalloproteinases expressions in Dengue virus-infected cells: implications for prevention of vascular permeability

Extracellular Matrix Protein Laminin Induces Matrix Metalloproteinase-9 in Human Breast Cancer Cell Line MCF-7

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