Multifunctional all-in-one adhesive hydrogel for the treatment of perianal infectious wounds

Abstract

Postoperative wound of perianal infectious disease represents a common but unique refractory wound in clinical practice. The reasons that hinder the wound healing process include not only the severe bacterial infection of the wound itself and the narrow and deep shape of the wound, but also its frequent bacterial contact. Therefore, the development of biofunctional dressings to aid in therapy is essential. In this study, we synthesized a new type of dressing comprising a hydrogel host based on the Schiff base principle and catechol groups between polydopamine, oxidized dextran, and quaternized chitosan, and then loaded it with traditional Chinese medicine molecules. These formed an integrated hydrogel for accelerated wound repair in a perianal infection model. The prepared hydrogels exhibited excellent wet tissue adhesion, antifouling, morphological variability, suitable swelling properties, and complete degradability, as well as remarkable contact antibacterial ability and the ability to rapidly scavenge free radicals. Hemostatic experiments showed excellent hemostatic properties, as the integrated hydrogel could instantly gel to seal the hemorrhage. Hemocompatibility and in vitro cell experiments showed that the integrated hydrogel had good biosafety and significantly promoted cell proliferation, which in turn accelerated the repair of infected whole cortexes in rats. A histomorphological evaluation showed that the integrated hydrogel promoted the recovery of normal anatomical tissue in rats by promoting the formation of collagen fibers and inhibiting inflammation. The results showed that this multifunctional integrated hydrogel has great potential for the treatment of continuously infected skin regeneration, providing a promising therapeutic strategy for postoperative wound healing in perianal infectious diseases.

MMP-Responsive Nanoparticle-Loaded, Injectable, Adhesive, Self-Healing Hydrogel Wound Dressing Based on Dynamic Covalent Bonds

“…AHADA/CCS/Col hydrogel (82.14 ± 18.65 μm) seemed denser when compared to other hydrogels (AHA/CCS with 133.58 ± 16.96 μm and AHADA/CCS with 119.19 ± 16.79 μm). The porous highly interconnected and multicross-linked network structure enables hydrogels to resemble strongly the extracellular matrix, enhancing their potential utility as wound dressing materials. , …”

“…Wound dressings with great tissue adhesion properties are ideal because they can firmly cover the surface of the wound and act as barrier protection. ,,− The hydrogel formed based on dynamic bonds was injectable and could be formed in situ on the tissue surface to better adapt to the position and shape of the wound. In order to measure the tissue adhesion of the hydrogels, we performed lap experiments (Figure A).…”

“…The porous highly interconnected and multicross-linked network structure enables hydrogels to resemble strongly the extracellular matrix, enhancing their potential utility as wound dressing materials. 32,33 Different hydrogel network structures correlate directly with their mechanical properties. As shown in Figure 2B, the hydrogel G′ remained stable within the studied frequency range.…”