Multifunctional 3D electrode platform for real-time in situ monitoring and stimulation of cardiac tissues

Zhang, Ning; Stauffer, Flurin; Simona, Benjamin R.; Zhang, Feng; Zhang, Zhaoming; Huang, Ning‐Ping; Vörös, János

doi:10.1016/j.bios.2018.04.037

Cited by 48 publications

(41 citation statements)

References 42 publications

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“…Living cells and tissues exhibit and respond to electrical potentials in the form of naturally occurring bioelectricity, important for tissue development and regeneration, or exogenously delivered electric current via, for example, electrodes as medical devices . Cellular responses include galvanotaxis, increased intracellular calcium concentration, extracellular matrix assembly, and associated cell proliferation and differentiation .…”

mentioning

confidence: 99%

Human Neural Tissues from Neural Stem Cells Using Conductive Biogel and Printed Polymer Microelectrode Arrays for 3D Electrical Stimulation

Tomaskovic-Crook

Zhang

Ahtiainen

et al. 2019

Adv Healthcare Materials

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Electricity is important in the physiology and development of human tissues such as embryonic and fetal development, and tissue regeneration for wound healing. Accordingly, electrical stimulation (ES) is increasingly being applied to influence cell behavior and function for a biomimetic approach to in vitro cell culture and tissue engineering. Here, the application of conductive polymer (CP) poly(3,4‐ethylenedioxythiophene)‐polystyrenesulfonate (PEDOT:PSS) pillars is described, direct‐write printed in an array format, for 3D ES of maturing neural tissues that are derived from human neural stem cells (NSCs). NSCs are initially encapsulated within a conductive polysaccharide‐based biogel interfaced with the CP pillar microelectrode arrays (MEAs), followed by differentiation in situ to neurons and supporting neuroglia during stimulation. Electrochemical properties of the pillar electrodes and the biogel support their electrical performance. Remarkably, stimulated constructs are characterized by widespread tracts of high‐density mature neurons and enhanced maturation of functional neural networks. Formation of tissues using the 3D MEAs substantiates the platform for advanced clinically relevant neural tissue induction, with the system likely amendable to diverse cell types to create other neural and non‐neural tissues. The platform may be useful for both research and translation, including modeling tissue development, function and dysfunction, electroceuticals, drug screening, and regenerative medicine.

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mentioning

confidence: 99%

Human Neural Tissues from Neural Stem Cells Using Conductive Biogel and Printed Polymer Microelectrode Arrays for 3D Electrical Stimulation

Tomaskovic-Crook

Zhang

Ahtiainen

et al. 2019

Adv Healthcare Materials

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“…Administration of verapamil, an L-type calcium channel blocker, which reduces calcium influx and action potential duration, resulted in a recorded decrease in the beating rate and contractility of cardiomyocytes, demonstrating the suitability of their heart-on-a-chip to evaluate drug effect. In another study, Zhang et al [ 52 ] developed a PDMS channel for 3D cardiac microtissue culture between two Pt-PDMS pillar electrodes to allow continuous electrical stimulation and impedance recording of local field potentials of cardiomyocytes ( Figure 7 b). The tissue was electrically stimulated through the Pt-PDMS electrodes by applying trains of bipolar electrical pulses (0.1 mA, 1 ms, 1 Hz, and 30 min/day for 6 days) that are characteristic for native myocardium [ 170 ].…”

Section: Biosensors For Measuring Ooc’ Electromechanical Activitymentioning

confidence: 99%

“…Adapted from Ref. [ 52 ] with permission from Elsevier. ( c ) Schematic and picture of the interdigitated electrodes (IDE)-based chip (i).…”

Section: Figurementioning

confidence: 99%

“…Specifically, a range of electrical, magnetic, and optical biosensing approaches have been reported over the last years and previously reviewed [ 42 , 43 , 44 , 45 ] for monitoring cell populations within microfabricated OoC systems, with high sensitivity and high resolution [ 46 , 47 , 48 , 49 ]. For instance, biosensors for monitoring cell growth and behavior [ 50 ], electrical [ 51 , 52 , 53 ] and mechanical [ 30 , 54 , 55 ] properties, as well as environmental parameters such as oxygen [ 56 , 57 , 58 ], pH [ 59 ], and metabolites concentration [ 60 , 61 ] have been studied and validated [ 62 ].…”

Section: Introductionmentioning

confidence: 99%

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Integrating Biosensors in Organs-on-Chip Devices: A Perspective on Current Strategies to Monitor Microphysiological Systems

et al. 2020

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Organs-on-chip (OoC), often referred to as microphysiological systems (MPS), are advanced in vitro tools able to replicate essential functions of human organs. Owing to their unprecedented ability to recapitulate key features of the native cellular environments, they represent promising tools for tissue engineering and drug screening applications. The achievement of proper functionalities within OoC is crucial; to this purpose, several parameters (e.g., chemical, physical) need to be assessed. Currently, most approaches rely on off-chip analysis and imaging techniques. However, the urgent demand for continuous, noninvasive, and real-time monitoring of tissue constructs requires the direct integration of biosensors. In this review, we focus on recent strategies to miniaturize and embed biosensing systems into organs-on-chip platforms. Biosensors for monitoring biological models with metabolic activities, models with tissue barrier functions, as well as models with electromechanical properties will be described and critically evaluated. In addition, multisensor integration within multiorgan platforms will be further reviewed and discussed.

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“…Therefore, as a minimal requirement for the functionality of the heart muscle, a responsive and contractile bundle of cardiac muscle fibers are needed. With this in mind, several design considerations have been taken into account when discussing heart-on-chip models, including the use of human induced pluripotent stem cells derived from cardiomyocytes, the addition of fibroblasts and endothelial cells for support, electrochemical stimulation, as well as real-time records of the contraction of the cardiac muscle [4][5][6].…”

Section: Heart-on-chipmentioning

confidence: 99%

Microfluidics – Organ-on-chip

Lungu

Grumezescu

2019

Biomed Eng Int

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This review is an introduction into the world of organ-on-chip models. By briefly explaining the concept of microfluidics and ‘lab-on-chip’, the main focus is on organs-on-chip and body-on-a-chip. The usual method to test the toxicity of a drug is through animal testing. However, the results do not always correlate to humans. In order to avoid animal testing, but also attain useful results, human-derived cell cultures using microfluidics have gained attention. Among all the different types of organ-on-chip devices, this review focuses on three distinct organs: heart, skin and liver. The main requirements for each organ-on-chip, as well as recent researches are presented. There have been considerable advancements with organ-on-chip models; however, even these have their limitations. Due to the fact that the system mimics a single organ, the systemic effect of drugs cannot be fully tested. Therefore, body-on-a-chip systems have been developed; which basically are a composed of a single chip that has several chambers, each chamber accounting for a distinct organ. Multi-organ-on-chip systems have been investigated, and even commercialized, the field still being under extensive research.

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Multifunctional 3D electrode platform for real-time in situ monitoring and stimulation of cardiac tissues

Cited by 48 publications

References 42 publications

Human Neural Tissues from Neural Stem Cells Using Conductive Biogel and Printed Polymer Microelectrode Arrays for 3D Electrical Stimulation

Human Neural Tissues from Neural Stem Cells Using Conductive Biogel and Printed Polymer Microelectrode Arrays for 3D Electrical Stimulation

Integrating Biosensors in Organs-on-Chip Devices: A Perspective on Current Strategies to Monitor Microphysiological Systems

Microfluidics – Organ-on-chip

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