Multifocal Electroretinogram in Rhodopsin P347L Transgenic Pigs

Abstract

In Tg retinas, ectopic synapses formed between cones and rod bipolar cells probably altered signal processing of the ON-bipolar cell pathway. In response to the altered visual signal input from the outer retina, signal processing in inner retinal neurons was also modified.

Electrophysiological Findings in a Porcine Model of Selective Retinal Capillary Closure

“…20,21 Pig eyes have been used as models for the study of the pathogenesis of retinal diseases, the testing of ophthalmic treatments, and surgical procedures. [22][23][24][25][26][27][28] An advantage of this novel pig model of retinal capillary closure is its unilaterality, for the closed end of the delivery catheter blocks the onward transmission of microspheres and prevents their systemic dissemination. Microspheres were absent from all the fellow control eyes reported here, thus enabling comparisons to be made between embolized and nonembolized eyes in the same animal.…”

Molecular complexes that direct rhodopsin transport to primary cilia

“…Modeling of ADRP mutations in transgenic animals has unequivocally confirmed the crucial role of this signal in the delivery of rhodopsin to the ROS. Transgenic mice, frogs, rats and pigs carrying mutations in the VxPx domain all develop retinal degeneration that is preceded by mislocalization of rhodopsin to multiple cellular compartments, including the photoreceptor synapse, which is normally completely devoid of rhodopsin (Concepcion & Chen, 2010; Concepcion et al, 2002; Green et al, 2000; Lee & Flannery, 2007; Li et al, 1996; Li et al, 1998; Ng et al, 2008; Sommer et al, 2011; Tam et al, 2000). The presence of mislocalized rhodopsin likely causes retinal degeneration in transgenic rods through the impairment of normal cellular functions that depend on the functional compartmentalization of photoreceptor cells.…”

The parameters of the porcine eyeball

“…The fundus reflection is orange to pale grey, with pigmented epithelial cells. The pig eye lacks a tapetum [32]. The retinal circulation is holangiotic [25].…”