Multifaceted nucleic acid probing with a rationally upgraded molecular rotor

Abstract

A rationally designed molecular rotor CCVJ-H shows superior performances than CCVJ-1 in a range of nucleic acid sensing applications, including nucleic acid detection, DNA glycosylase assay, and nucleic acid-small molecule interaction profiling.

Merging DNA Repair with Bioorthogonal Conjugation Enables Accessible and Versatile Asymmetric DNA Catalysis

“…The bioorthogonal reactivity of these aldehyde sites generated by UDG has been previously exploited for site-selective attachment of oxyamine, hydrazine, or hydrazide-based chemical probes for biological and biomedical applications. However, this strategy has not been explored for attachment of catalysts. − Notably, both dU-containing DNA and E. coli UDG are cheaply and widely available, potentially allowing systematic investigations into the DNA sequence effect on reaction outcomes in a high-throughput format as well as permitting site-specific modification of long and complex DNA scaffolds which are otherwise challenging using phosphoramidite chemistry.…”