Multiepitope Proteins for the Differential Detection of IgG Antibodies against RBD of the Spike Protein and Non-RBD Regions of SARS-CoV-2

Gomes, Larissa Rodrigues; Durans, Andressa da Matta; Napoleão-Pêgo, Paloma; Waterman, Jessica A.; Freitas, Mariana S.; Sá, Nathalia Beatriz Ramos de; Pereira, Lilian V.; Furtado, Jéssica S.; Aquino, Romário G.; Machado, Mario C. R.; Fintelman-Rodrigues, Natália; Souza, Thiago Moreno L.; Morel, Carlos Médicis; Provance, David William; De-Simone, Salvatore Giovanni

doi:10.3390/vaccines9090986

Cited by 12 publications

(15 citation statements)

References 56 publications

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“…Determining the actual burden of infection is also vital for epidemic forecasting and response planning. Seroprevalence studies are potent in identifying the number of undiagnosed missing cases with mild or no symptoms or who cannot undergo testing, which may contribute significantly to the transmission [ 7 , 8 , 9 , 10 , 11 ]. Furthermore, seroprevalence studies estimate the susceptible population in a community.…”

Section: Introductionmentioning

confidence: 99%

Seroprevalence of Anti-SARS-CoV-2 Antibodies in Chattogram Metropolitan Area, Bangladesh

et al. 2022

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Seroprevalence studies of COVID-19 are used to assess the degree of undetected transmission in the community and different groups such as health care workers (HCWs) are deemed vulnerable due to their workplace hazards. The present study estimated the seroprevalence and quantified the titer of anti-SARS-CoV-2 antibody (IgG) and its association with different factors. This cross-sectional study observed HCWs, in indoor and outdoor patients (non-COVID-19) and garment workers in the Chattogram metropolitan area (CMA, N = 748) from six hospitals and two garment factories. Qualitative and quantitative ELISA were used to identify and quantify antibodies (IgG) in the serum samples. Descriptive, univariable, and multivariable statistical analysis were performed. Overall seroprevalence and among HCWs, in indoor and outdoor patients, and garment workers were 66.99% (95% CI: 63.40–70.40%), 68.99% (95% CI: 63.8–73.7%), 81.37% (95% CI: 74.7–86.7%), and 50.56% (95% CI: 43.5–57.5%), respectively. Seroprevalence and mean titer was 44.47% (95% CI: 38.6–50.4%) and 53.71 DU/mL in the non-vaccinated population, respectively, while it was higher in the population who received a first dose (61.66%, 95% CI: 54.8–68.0%, 159.08 DU/mL) and both doses (100%, 95% CI: 98.4–100%, 255.46 DU/mL). This study emphasizes the role of vaccine in antibody production; the second dose of vaccine significantly increased the seroprevalence and titer and both were low in natural infection.

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Section: Introductionmentioning

confidence: 99%

Seroprevalence of Anti-SARS-CoV-2 Antibodies in Chattogram Metropolitan Area, Bangladesh

et al. 2022

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“…B-cell epitope mapping plays a key role in robust immune response induction. This is due to the important role of B-cells in the regulation of an immune response through the production of antibodies against the invading pathogen [ 30 , 31 ]. This B-cell response is triggered by the identification of epitope sequences by the antigen.…”

Section: Resultsmentioning

confidence: 99%

Potential Immunogenic Activity of Computationally Designed mRNA- and Peptide-Based Prophylactic Vaccines against MERS, SARS-CoV, and SARS-CoV-2: A Reverse Vaccinology Approach

Khan

Ansari

et al. 2022

Molecules

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The continued emergence of human coronaviruses (hCoVs) in the last few decades has posed an alarming situation and requires advanced cross-protective strategies against these pandemic viruses. Among these, Middle East Respiratory Syndrome coronavirus (MERS-CoV), Severe Acute Respiratory Syndrome coronavirus (SARS-CoV), and Severe Acute Respiratory Syndrome coronavirus-2 (SARS-CoV-2) have been highly associated with lethality in humans. Despite the challenges posed by these viruses, it is imperative to develop effective antiviral therapeutics and vaccines for these human-infecting viruses. The proteomic similarity between the receptor-binding domains (RBDs) among the three viral species offers a potential target for advanced cross-protective vaccine designs. In this study, putative immunogenic epitopes including Cytotoxic T Lymphocytes (CTLs), Helper T Lymphocytes (HTLs), and Beta-cells (B-cells) were predicted for each RBD-containing region of the three highly pathogenic hCoVs. This was followed by the structural organization of peptide- and mRNA-based prophylactic vaccine designs. The validated 3D structures of these epitope-based vaccine designs were subjected to molecular docking with human TLR4. Furthermore, the CTL and HTL epitopes were processed for binding with respective human Lymphocytes Antigens (HLAs). In silico cloning designs were obtained for the prophylactic vaccine designs and may be useful in further experimental designs. Additionally, the epitope-based vaccine designs were evaluated for immunogenic activity through immune simulation. Further studies may clarify the safety and efficacy of these prophylactic vaccine designs through experimental testing against these human-pathogenic coronaviruses.

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“…coli cells Indirect ELISA 147 foot-and-mouth-positive serum samples/610 healthy swine serum samples Sensitivity: 95.9% Specificity: 96.7% [ 105 ]/China Human hepatitis C/hepatitis C virus Recombinant multiepitope HCV antigen E . coli cells ELISA 88 hepatitis-positive serum samples/376 hepatitis C-negative serum samples/18 serum samples from non-hepatitis C diseases Sensitivity: 100% Specificity: 99.73% [ 106 ]/Brazil Human cytomegalovirus/cytomegalovirus rMEHCMV E. coli BL21(DE3) cells ELISA 12 cytomegalovirus-positive serum samples/1 serum sample from healthy individual rMEHCMV was recognized by human positive samples [ 107 ]/Brazil Canine coronavirus/SARS-CoV-2 rSP E. coli BL21(DE3) cells Indirect ELISA 64 coronavirus-positive serum samples/10 healthy dog serum samples 82.81% positive rate [ 108 ]/China Human coronavirus/SARS-CoV-2 Dx-SARS2-RBD and Dx-SARS2-noRBD E. coli BL21(DE3) cells ELISA 185 coronavirus-positive serum samples/256 serum samples from healthy individuals/94 serum samples from non-coronovirus diseases Dx-SARS2-RBD Sensitivity: 100% Specificity: 99.51-100% Dx-SARS2-noRBD Sensitivity: 100% Specificity: 99.21-100% [ 109 ]/Brazil African swine fever/African swine fever virus reMeP72 E. coli BL21(DE3) cells Colloidal gold-based immunochromatographic assay 139 swine serum samples Sensitivity: 85.7% Specificity: 97.6% [ 110 ]/China …”

Section: Rmps Applied In Disease Diagnosismentioning

confidence: 99%

Recombinant multiepitope proteins expressed in Escherichia coli cells and their potential for immunodiagnosis

Gonçalves,

Ribeiro,

Resende

et al. 2024

Microb Cell Fact

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Recombinant multiepitope proteins (RMPs) are a promising alternative for application in diagnostic tests and, given their wide application in the most diverse diseases, this review article aims to survey the use of these antigens for diagnosis, as well as discuss the main points surrounding these antigens. RMPs usually consisting of linear, immunodominant, and phylogenetically conserved epitopes, has been applied in the experimental diagnosis of various human and animal diseases, such as leishmaniasis, brucellosis, cysticercosis, Chagas disease, hepatitis, leptospirosis, leprosy, filariasis, schistosomiasis, dengue, and COVID-19. The synthetic genes for these epitopes are joined to code a single RMP, either with spacers or fused, with different biochemical properties. The epitopes’ high density within the RMPs contributes to a high degree of sensitivity and specificity. The RMPs can also sidestep the need for multiple peptide synthesis or multiple recombinant proteins, reducing costs and enhancing the standardization conditions for immunoassays. Methods such as bioinformatics and circular dichroism have been widely applied in the development of new RMPs, helping to guide their construction and better understand their structure. Several RMPs have been expressed, mainly using the Escherichia coli expression system, highlighting the importance of these cells in the biotechnological field. In fact, technological advances in this area, offering a wide range of different strains to be used, make these cells the most widely used expression platform. RMPs have been experimentally used to diagnose a broad range of illnesses in the laboratory, suggesting they could also be useful for accurate diagnoses commercially. On this point, the RMP method offers a tempting substitute for the production of promising antigens used to assemble commercial diagnostic kits.

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Multiepitope Proteins for the Differential Detection of IgG Antibodies against RBD of the Spike Protein and Non-RBD Regions of SARS-CoV-2

Cited by 12 publications

References 56 publications

Seroprevalence of Anti-SARS-CoV-2 Antibodies in Chattogram Metropolitan Area, Bangladesh

Seroprevalence of Anti-SARS-CoV-2 Antibodies in Chattogram Metropolitan Area, Bangladesh

Potential Immunogenic Activity of Computationally Designed mRNA- and Peptide-Based Prophylactic Vaccines against MERS, SARS-CoV, and SARS-CoV-2: A Reverse Vaccinology Approach

Recombinant multiepitope proteins expressed in Escherichia coli cells and their potential for immunodiagnosis

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