Multienvironmental evaluation of wheat (Triticum aestivum L.) germplasm identifies donors with multiple fungal disease resistance

Kumar, Satish; Singroha, Garima; Bhardwaj, Subhash; Bala, Ritu; Saharan, M. S.; Gupta, Vikas; Khan, Anwar; Mahapatra, Sunita; Sivasamy, M.; Rana, Vijay; Mishra, C. N.; Prakash, Om; Verma, Ajay; Sharma, Pradeep; Sharma, Indu; Chatrath, Ravish; Singh, Gyanendra Pratap

doi:10.1007/s10722-019-00751-3

Cited by 20 publications

(8 citation statements)

References 20 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Genetics of Karnal bunt resistance indicates that the resistance is governed by one to several genes having partial dominance [20,21,22,23,24,25]. Some of the Indian, Chinese and Brazilian wheat have been reported to have sufficient levels of resistance to Karnal bunt [26,27]. Besides cultivated germplasm, several accessions of Aegilops species [28] and synthetic hexaploid wheat (derived from hybridization of T. durum and Ae.…”

Section: Introductionmentioning

confidence: 99%

Genome Wide Association Study of Karnal Bunt Resistance in a Wheat Germplasm Collection from Afghanistan

Gupta

Kumar

et al. 2019

IJMS

Self Cite

View full text Add to dashboard Cite

Karnal bunt disease of wheat, caused by the fungus Neovossia indica, is one of the most important challenges to the grain industry as it affects the grain quality and also restricts the international movement of infected grain. It is a seed-, soil- and airborne disease with limited effect of chemical control. Currently, this disease is contained through the deployment of host resistance but further improvement is limited as only a few genotypes have been found to carry partial resistance. To identify genomic regions responsible for resistance in a set of 339 wheat accessions, genome-wide association study (GWAS) was undertaken using the DArTSeq® technology, in which 18 genomic regions for Karnal bunt resistance were identified, explaining 5–20% of the phenotypic variation. The identified quantitative trait loci (QTL) on chromosome 2BL showed consistently significant effects across all four experiments, whereas another QTL on 5BL was significant in three experiments. Additional QTLs were mapped on chromosomes 1DL, 2DL, 4AL, 5AS, 6BL, 6BS, 7BS and 7DL that have not been mapped previously, and on chromosomes 4B, 5AL, 5BL and 6BS, which have been reported in previous studies. Germplasm with less than 1% Karnal bunt infection have been identified and can be used for resistance breeding. The SNP markers linked to the genomic regions conferring resistance to Karnal bunt could be used to improve Karnal bunt resistance through marker-assisted selection.

show abstract

Section: Introductionmentioning

confidence: 99%

Genome Wide Association Study of Karnal Bunt Resistance in a Wheat Germplasm Collection from Afghanistan

Gupta

Kumar

et al. 2019

IJMS

Self Cite

View full text Add to dashboard Cite

show abstract

“…FAO (2018FAO ( , 2019 predicts an additional requirement of 198 million tonnes of wheat by 2050. In order to achieve the required levels, wheat production needs to be increased sufficiently in developing countries (Kumar et al 2019) Wheat is very susceptible to heat and drought at reproductive and grain filling stage (Alexander et al, 2006;Gaffen and Ross 1998;Hennessy et al, 2008).…”

Section: Introductionmentioning

confidence: 99%

Validation of molecular markers linked with QTLs for heat and drought stress tolerance in wheat

Singroha¹,

Mishra²,

Kumar³

et al. 2021

Journal of Cereal Research

Self Cite

View full text Add to dashboard Cite

Global climatic changes affect the production and productivity of wheat around the world. Increasing heat and drought are the most important abiotic stress factors that hinder productivity. To produce sufficient wheat there is urgent need to develop heat and drought tolerant wheat cultivars; therefore, identification of genetic diversity present among available wheat genotypes using SSRs markers is of prime importance. The allelic variation present among wheat genotypes can be deployed in breeding heat and drought tolerant cultivars. A set of 36 wheat genotypes was evaluated for evaluating genetic diversity for heat and drought tolerance among wheat genotypes using already reported SSR markers. A total of 24 SSR markers were analysed and out of them 19 were found polymorphic. Using SSR markers data cluster analysis was carried out to classify the wheat cultivars for genetic variation in heat tolerance. The dendrogram constructed using SSR markers data assigned the 36 wheat cultivars into three broad clusters. The polymorphic SSRs thus identified could be used to evaluate genetic diversity present among wheat genotypes and the wheat cultivars thus identified could be exploited in wheat breeding programs.

show abstract

“…Distribution and diversity of pathogenic population over a large area is an essential criterion for disease management (Kumar et al 2019;Mahapatra and Das 2013;Rampersad et al 2013;McDonald and Linde 2002). Therefore, the current research is the rst attempt to (i) analyze the genetic and phylogenetic relationships between ve different populations of B. sorokiniana isolates from wheat growing zones of India and (ii) elucidate their demographic expansion by generating and comparing ITS gene sequence data.…”

Section: Introductionmentioning

confidence: 99%

Molecular Diversity, Haplotype Distribution and Gene Flow of Bipolaris Sorokiniana Fungus Causing Spot Blotch Disease in Different Wheat Growing Zones

Kashyap¹,

Kumar²,

Sharma³

et al. 2021

Preprint

Self Cite

View full text Add to dashboard Cite

Bipolaris sorokiniana (BS) is an economically important fungal pathogen causing spot blotch of wheat (Trtiticum aestivum) and found in all wheat growing zones of India. Very scanty and fragmentary information is available on its genetic diversity. The current research is the first detailed report on the geographic distribution and evolution of BS population in five geographically distinct wheat growing zones [North Western Plain Zone (NWPZ), North Eastern Plain zone (NEPZ); North Hill Zone (NHZ), Southern Hill Zone (SHZ) and Peninsular Zone (PZ)] of India, studied by performing nucleotide sequence comparison of internal transcribed spacer region of 183 isolates. A moderate to high levels of haplotypic diversity was noticed in different wheat growing zones. Phylogenetic analysis suggests that B. sorokiniana exist in two distinct lineages as all isolates under study were grouped in two different clades and found analogous to the findings of haplotypic and median joining network analysis. The genetic parameters revealed the existence of 59 haplotypes with three major haplotypes (H_2, H_3, and H_25) which showed star-like structure network surrounded by several single haplotypes, revealing high frequency of the mutations ((Eta = 2 – 437) in total analyzed population. H_3 was observed as a predominant haplotype and prevalent in all the five zones. Moderate level of genetic differentiation was found between NEPZ and PZ (Fst = 0.563), whereas it was low between NEPZ and NHZ (Fst = -0.062). High level of gene flow was noticed between NWPZ and NEPZ (Nm = 14.32), while it was found minimum between SZ and NHZ (Nm = 0.50). Moreover, negative score of neutrality statistics (Tajima’s D and Fu’s FS test) for NWPZ, PZ and SHZ populations, suggested recent population expansion in these zones. However, positive score for both the neutrality tests observed in NEPZ and NHZ indicated the dominance of balancing selection in structuring their population. Recombination events were observed in the NWPZ, NEPZ and NHZ population, while it was absent in SHZ and PZ population. Thus, the lack of any specific genetic population structure in all the zones indicates for the expansion history only from one common source population i.e. NWPZ, a mega zone of wheat production in India. Overall, it seems that the predominance of individual haplotypes with a moderate level of genetic variation and men mediated movement of contaminated seed and dispersal of inoculum, mutations and recombination as prime evolutionary processes play essential role in defining the genetic structure of BS population.

show abstract

Multienvironmental evaluation of wheat (Triticum aestivum L.) germplasm identifies donors with multiple fungal disease resistance

Cited by 20 publications

References 20 publications

Genome Wide Association Study of Karnal Bunt Resistance in a Wheat Germplasm Collection from Afghanistan

Genome Wide Association Study of Karnal Bunt Resistance in a Wheat Germplasm Collection from Afghanistan

Validation of molecular markers linked with QTLs for heat and drought stress tolerance in wheat

Molecular Diversity, Haplotype Distribution and Gene Flow of Bipolaris Sorokiniana Fungus Causing Spot Blotch Disease in Different Wheat Growing Zones

Contact Info

Product

Resources

About