Multielemental determination in breast cancerous and non-cancerous biopsies by inductively coupled plasma-mass spectrometry following small volume microwave-assisted digestion

Elemental fingerprinting of tumorous and adjacent non-tumorous tissues from patients with colorectal cancer using ICP-MS, ICP-OES and chemometric analysis

“…Microwave sample digestions were performed in the PFA Teflon vials (6 ml) with 0.3 ml of HNO 3 following the procedure described elsewhere (Millos et al 2008). Three subsamples of each biopsy (20-30 mg mass) were digested.…”

Low volume microwave digestion and direct determination of selenium in biological samples by hydride generation-atomic fluorescence spectrometry

“…In 2000, Rodushkin and colleagues determined 60 elements in whole human blood sample by ICP-MS after a LVMWD with only one mL of sample [16]. In the recent years, several groups have reported multi-elemental determination of human biopsies based on LVMWD technique [17][18][19][20]. All these studies were performed with ICP-OES (optical emission spectrometry) [20], ICP-MS [18,19] or both ICP-MS and TXRF (total reflection X-ray fluorescence) spectrometry [17].…”

“…In the recent years, several groups have reported multi-elemental determination of human biopsies based on LVMWD technique [17][18][19][20]. All these studies were performed with ICP-OES (optical emission spectrometry) [20], ICP-MS [18,19] or both ICP-MS and TXRF (total reflection X-ray fluorescence) spectrometry [17]. With these techniques, samples do not require a complete digestion, as organic colloids are readily transformed after digestion into elemental forms in the high temperature flame, graphite tube and plasma torch before being determined spectrometrically.…”

Liver Biopsy

“…The suitability of histochemical staining methods and two microanalytical methods were studied. Inductively coupled plasma mass spectrometry (ICP-MS) has favourable detection limits and was selected for simultaneous determination of micro and trace elements (Labat, et al, 2003;Millos, et al, 2008). Total reflection X-ray fluorescence spectrometry (TXRF) is also a suitable and powerful technique for analysis of small-mass biopsy samples, because it requires small amount of substance (Marco, et al, 2004).…”