Multielemental Analysis of Bee Pollen, Propolis, and Royal Jelly Collected in West-Central Poland

Matuszewska, Eliza; Klupczynska, Agnieszka; Maciołek, Krzysztof; Kokot, Zenon J.; Matysiak, Jan

doi:10.3390/molecules26092415

Cited by 31 publications

(20 citation statements)

References 92 publications

(115 reference statements)

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Each sample was analyzed in seven replicates. A detailed description of the sample preparation process and the outcome of the validation study were presented in a previous study [ 13 ].…”

Section: Methodsmentioning

confidence: 99%

“…This study used the ED-XRF technology to analyze the most abundant and essential minerals (K, P, Ca, Zn, Mn, Fe, Cu, and S) in bee pollen, vital for human health and well-being. Recently, our group has published a paper describing the use of ICP-MS and ICP-OES to analyze a large group of bee pollen samples, including propolis, bee pollen, and royal jelly [ 13 ]. However, compared with the ICP methods, ED-XRF is much faster and does not require a tedious sample preparation.…”

Section: Introductionmentioning

confidence: 99%

“…For this purpose, we analyzed bee pollen samples collected in Poland (Greater Poland region) in 2018 and 2019 and a group of commercially available products. Two samples, first examined using ICP-MS and ICP-OES [ 13 ], were used as standard samples to develop a proper ED-XRF method. Two different ED-XRF approaches were tested.…”

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Rapid and Accurate Approach for Honeybee Pollen Analysis Using ED-XRF and FTIR Spectroscopy

et al. 2021

Self Cite

View full text Add to dashboard Cite

Since honeybee pollen is considered a “perfectly complete food” and is characterized by many beneficial properties (anti-inflammatory, antioxidant, anti-bacterial, etc.), it has begun to be used for therapeutic purposes. Consequently, there is a high need to develop methods for controlling its composition. A thorough bee pollen analysis can be very informative regarding its safety for consumption, the variability of its composition, its biogeographical origin, or harvest date. Therefore, in this study, two reliable and non-destructive spectroscopy methods, i.e., ED-XRF and ATR–FTIR, are proposed as a fast approach to characterize bee pollen. The collected samples were derived from apiaries located in west-central Poland. Additionally, some commercially available samples were analyzed. The applied methodology was optimized and combined with sophisticated chemometric tools. Data derived from IR analyses were also subjected to two-dimensional correlation spectroscopy. The developed ED-XRF method allowed the reliable quantification of eight macro- and micro-nutrients, while organic components were characterized by IR spectroscopy. Principal component analysis, cluster analysis, and obtained synchronous and asynchronous maps allowed the study of component changes occurring dependently on the date and location of harvest. The proposed approach proved to be an excellent tool to monitor the variability of the inorganic and organic content of bee pollen.

show abstract

“…Each sample was analyzed in seven replicates. A detailed description of the sample preparation process and the outcome of the validation study were presented in a previous study [ 13 ].…”

Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Rapid and Accurate Approach for Honeybee Pollen Analysis Using ED-XRF and FTIR Spectroscopy

et al. 2021

Self Cite

View full text Add to dashboard Cite

show abstract

“…Studies reporting elemental analysis of bee pollen are abundant. It is recognized that the content of macroelements (K, P, S, Ca and Mg, followed by Na, Fe, Mn, Al and Zn) as well as microelements and trace elements can vary in bee pollen considerably [42,43]. On the contrary, works describing NHC analysis are scarce.…”

Section: Modeling Of Nitrogen Carbon and Sulfur Contentmentioning

confidence: 99%

Modeling of Antioxidant Activity, Polyphenols and Macronutrients Content of Bee Pollen Applying Solid-State 13C NMR Spectra

et al. 2021

View full text Add to dashboard Cite

An application of solid 13C nuclear magnetic resonance (NMR) spectroscopy for the determination of macronutrients, total polyphenols content, antioxidant activity, N C S elements, and pH in commercially available bee pollens is reported herein. Solid-state 13C NMR spectra were recorded for homogenized pollen granules without chemical treatment or dissolution of samples. By combining spectral data with the results of reference analyses, partial least squares models were constructed and validated separately for each of the studied parameters. To characterize and compare the models’ quality, the relative standard errors of prediction (RSEP) were calculated for calibration and validation sets. In the case of the analysis of protein, fat and reducing sugars, these errors were in the 1.8–2.5% range. Modeling the elemental composition of bee pollen on the basis of 13C NMR spectra resulted in RSEPcal/RSEPval values of 0.3/0.6% for the sum of NHCS elements, 0.3/0.4% for C, 1.8/1.9% for N, and 4.2/6.1% for S quantification. Analyses of total phenolics and ABTS antioxidant activity resulted in RSEP values in the 2.7–3.5% and 2.8–3.8% ranges, respectively, whereas they were 1.4–2.1% for pH. The obtained results demonstrate the usefulness of 13C solid-state NMR spectroscopy for direct determination of various important physiochemical parameters of bee pollen.

show abstract

“…Мінеральне живлення медоносних бджіл, як і ссавців, забезпечується надходженням у травний тракт життєвоважливих елементів, фізіологічна роль яких встановлена для організму (Mg, Ca, Fe, Zn, Cо, Cu, Mn, I, Se) з природного корму -пилку і нектару або паді (Romaniv & Кovalchuk, 2012;Кovalchuk et al, 2014; та води (Matuszewska et al, 2021). Разом з тим живлення бджіл у природних умовах з інтенсивним техногенним навантаженням на довкілля зумовлює негативний вплив на їхню життєдіяльність (Кovalchuk, 2012;Zhumadina et al, 2015;DeGrandi-Hoffman et al, 2018;Pashaian et al, 2021).…”

Section: вступunclassified

The influence of nanotechnological preparations of plant protection on the viability of bees and the content of microelements in their tissues

et al. 2021

View full text Add to dashboard Cite

The research was performed in terms of keeping bees in an incubator. Bees were selected families of analog and divided into control (І) and 6 experimental (ІІ-ІV) groups. Over 7 days, bees of control and experimental groups were fed 1 ml sugar syrup (SS), adding to that amount of citrate of polielemental nanotechnology drugs recommended for plant protection – “Avatar-Insecticide” (“A-I”) and “Avatar-Fungicide” (“A-F”) in a dilution of 1:1000 (II, III groups) and 1: 500 (IV, V groups) and their VI compatible combination (1:1000) and VII (1:500) groups. Every day number of live and dead bees in groups in absolute and relative values were counted. For 7 th day of feeding bees, drugs were taken to determine Co Zn, Cu, Ag in tissues. The results were statistically processed with the use of Microsoft Excel. The aim of the research is safety for beekeeping use of experimental drugs for plant protection “A-F” and “A-I” the conditions of feeding their bees. The differences of influence of drugs “A-F” and “A-I” on safety and loss of bees, their content in tissues Co and Cu depending on concentration, method, and duration of use were shown. The significant positive effect of feeding “A-F” drug dilution of 1:1000 on the safety of bees with the increase in the number of living by 12.7 % on day 7 compared to the control was noted. Application of this the drug in a dilution of 1:500 and its mixture with “A-I” in a dilution of 1:1000 and 1:500 reduced rates of preservation of bees throughout the research period. Separate feeding preparation of “A-F” and “A-I” compatible with dilutions of 1:1000 and 1:500 resulted in the highest content of Cо in the tissues of bees. Noted lower Cu content in tissues bee research groups at higher levels amid Zn and Ag by joint action both drugs in a dilution of 1:500 compared to the control. The results indicate a toxic effect of “A-I” drug to bees in the laboratory employed in concentrations and combinations and drug “A-F” – only a dilution of 1:500. Found high the ability of the cumulative drug doses applied “A-F” content of Co tissues of bees.

show abstract

Multielemental Analysis of Bee Pollen, Propolis, and Royal Jelly Collected in West-Central Poland

Cited by 31 publications

References 92 publications

Rapid and Accurate Approach for Honeybee Pollen Analysis Using ED-XRF and FTIR Spectroscopy

Rapid and Accurate Approach for Honeybee Pollen Analysis Using ED-XRF and FTIR Spectroscopy

Modeling of Antioxidant Activity, Polyphenols and Macronutrients Content of Bee Pollen Applying Solid-State 13C NMR Spectra

The influence of nanotechnological preparations of plant protection on the viability of bees and the content of microelements in their tissues

Contact Info

Product

Resources

About