Multidynamic Osteogenic Differentiation by Effective Polydopamine Micro-Arc Oxide Manipulations

Abstract

Introduction The nanostructural modification of the oral implant surface can effectively mimic the morphology of natural bone tissue, allowing osteoblasts to achieve both proliferation and differentiation capabilities at the bone interface of the dental implant. To improve the osteoinductive activity on the surface of titanium implants for rapid osseointegration, we prepared a novel composite coating (MAO-PDA-NC) by micro-arc oxidation technique and immersion method and evaluated the proliferation, adhesion, and osteogenic differentiation of osteoblasts on this coating. Methods The coatings were prepared by micro-arc oxidation (MAO) technique and immersion method, and characterized by scanning electron microscopy (SEM) and atomic force microscopy (AFM) for different coatings; the loading of PDA was examined using Fourier transform infrared spectroscopy (FTIR); the ion release capacity of the coatings was determined by inductively coupled plasma emission spectrometry (ICP-OES); the interfacial bonding of the coatings was examined using nanoscratch experiment strength. The cytotoxicity of the coating was examined by live/dead staining kit; cell proliferation viability was examined by CCK-8 kit; adhesion and osteogenic effect of the coating were examined by immunofluorescence staining and RT-PCR; osteogenic differentiation was examined by alkaline phosphatase staining. Results The surface morphology of titanium implants was modified by micro-arc oxidation technology, and a new MAO-PDA-NC composite coating was successfully prepared. The results showed that the MAO-PDA-NC coating not only optimized the physical and chemical properties of the titanium implant surface but also significantly stimulated the biological properties of osteoblast adhesion, proliferation, and osteogenic differentiation on the coating surface. Conclusion These results show that MAO-PDA-NC composite coating can significantly improve the surface properties of titanium implants and achieve a stable bond between implant and bone tissue, thus accelerating early osseointegration.

Effect of micro-arc oxidation coatings with graphene oxide and graphite on osseointegration of titanium implants-an in vivo study

“…MAO applied to titanium implants before surface coatings has enhanced chemical reactions at the implant surface, promoting improved osteoblast adhesion, proliferation, and osteogenic differentiation. This enhancement improves osseointegration between the implant and bone ( Zhou et al, 2022 ).…”

Fabrication and in vitro biological performance of a double-layered nanoparticles-microarc oxidation composite coating on titanium for dental implant application

“…Minocycline-loaded nanomicelles were incorporated into the pores of MAO treated titanium (MAO-Ti), and the composite coating showed excellent antibacterial ability [20]. Zhou fabricated a composite dopamine-nanoclay coating on MAO-Ti by immersion method, which could significantly stimulate osteoblast adhesion, proliferation, and osteogenic differentiation [21]. A bioactive silicon/copper-MAO composite coating was fabricated by doping silicon and copper ions into MAO coating, and the coating presented good antibacterial and osteogenic properties [22].…”