Multidrug-Resistant Klebsiella variicola Isolated in the Urine of Healthy Bovine Heifers, a Potential Risk as an Emerging Human Pathogen

Giannattasio-Ferraz, Silvia; Ene, Adriana; Johnson, Genevieve; Maskeri, Laura; Oliveira, André Alves de; Banerjee, Swarnali; Barbosa-Stancioli, Edel Figueiredo; Putonti, Catherine

doi:10.1128/aem.00044-22

Cited by 4 publications

(3 citation statements)

References 59 publications

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“…25 As well, the circulation of multidrug-resistant K. variicola in healthy livestock animals has also been reported. 26 Contrary to the existing studies that investigate the role of K. variicola in environmental resistance and animal disease, there is growing evidence of its clinical impact. Previous research has demonstrated that K. variicola is associated with a range of infections, including bloodstream infections, urinary infections, and respiratory tract infections.…”

Section: Discussionmentioning

confidence: 99%

Emergence of OXA-484-Producing Klebsiella variicola in China

Ge¹,

Qiao²,

Xu³

et al. 2023

IDR

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The frequent and inappropriate use of antibiotics has caused a dramatic rise in the number, species, and degree of multi-drug resistant bacteria, making them more prevalent and difficult to treat. In this context, the aim of the present study was to characterize the OXA-484-producing strains isolated from a perianal swab of a patient by using whole-genome analysis. Patients and Methods: In this study, carbapenemase-producing Klebsiella variicola was identified by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS), average nucleotide identity (ANI) and PCR. S1 nuclease pulsed-field gel electrophoresis (S1-PFGE) and Southern blotting were utilized to characterize the plasmid profiles of K. variicola 4717. In particular, WGS was performed to obtain genomic information on this clinical isolate, and assemble all the plasmids of the bla OXA-484 -harboring strain. Results: The antimicrobial susceptibility pattern of K. variicola 4717 revealed that it was resistant to a range of antibiotics, including aztreonam, imipenem, meropenem, ceftriaxone, cefotaxime, ceftazidime, levofloxacin, ciprofloxacin, piperacillin-tazobactam, methylenesulfamer oxazole, amoxicillin-clavulanic acid, cefepime, and tigecycline. Its susceptibility to chloromycin was intermediate, while it was still susceptible to amikacin, gentamicin, fosfomycin, and polymyxin B. The presence of two companion plasmids, p4717_1 and p4717_2, together with a plasmid carrying the bla OXA-484 gene was observed. An in-depth investigation of p4717-OXA-484 uncovered that it is an IncX3-type plasmid and shares a similar segment encoded by IS26. Given the similar genetic background, it was conceivable that bla OXA-484 could have developed from bla OXA-181 through a series of mutations. Conclusion: Herein, we described the first genome sequence of K. variicola strain harbouring the class D β-actamase bla OXA-484 in an Inc-X3-type plasmid. Our work also uncovered the genetic characterization of K. variicola 4717 and the importance of initiating antimicrobial detection promptly.

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Section: Discussionmentioning

confidence: 99%

Emergence of OXA-484-Producing Klebsiella variicola in China

Ge¹,

Qiao²,

Xu³

et al. 2023

IDR

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“…While Klebsiella pneumoniae species complex (KpSC) members are clinically relevant as opportunistic human pathogens ( 1 ) with intrinsic resistance to penicillins ( 2 , 3 ) often extending to cephalosporins ( 4 , 5 ) and carbapenems ( 6 , 7 ), they are also found as endophytes ( 8 ). Kingdom-crossing is especially pronounced in strains of K. variicola ( 9 ), which are not only nitrogen fixers and plant colonizers, isolated from sources such as sugarcane ( 10 ), rice and bananas ( 11 ), but also found in bloodstream infections ( 12 ), cattle urine ( 13 ) and termite gut microbiota ( 14 ).…”

Section: Announcementmentioning

confidence: 99%

Complete genome sequence of Klebsiella variicola subsp. variicola ML.9ba2, an endophytic strain isolated from aerial roots of Philodendron erubescens

Lam,

Leung,

Lai

et al. 2024

Microbiol Resour Announc

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“…The latter is commonly isolated as part of plant microbiomes as it can promote plant growth by nitrogen fixation (Pinto-Tomás et al 2009 ). It is also an emerging human pathogen (Rodríguez-Medina et al 2019 ) causing urinary tract infections (Potter et al 2018 ), as well as in other diseases in wild and farm animals (Giannattasio-Ferraz et al 2022 ). However, K. variicola has been largely neglected due to a historic taxonomic misclassification (Martínez-Romero et al 2018 , Rodrigues et al 2018 ).…”

Section: Introductionmentioning

confidence: 99%

Emergence of novel non-aggregative variants under negative frequency-dependent selection in Klebsiella variicola

Nucci,

Janaszkiewicz,

Rocha

et al. 2023

microLife

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Klebsiella variicola is an emergent human pathogen causing diverse infections, some of which in the urinary tract. However, little is known about the evolution and maintenance of genetic diversity in this species, the molecular mechanisms and their population dynamics. Here, we characterized the emergence of a novel rdar-like (rough and dry) morphotype which is contingent both on the genetic background and the environment. We show that mutations in either the nitrogen assimilation control gene (nac) or the type III fimbriae regulator, mrkH, suffice to generate rdar-like colonies. These morphotypes are primarily selected for the reduced inter-cellular aggregation as a result of MrkH loss-of-function which reduces type 3 fimbriae expression. Additionally, these clones also display increased growth rate and reduced biofilm formation. Direct competitions between rdar and wild type clones show that mutations in mrkH provide large fitness advantages. In artificial urine, the morphotype is under strong negative frequency-dependent selection and can socially exploit wild type strains. An exhaustive search for mrkH mutants in public databases revealed that ca 8% of natural isolates analysed had a truncated mrkH gene many of which were due to insertions of IS elements, including a reported clinical isolate with rdar morphology. These strains were rarely hypermucoid and often isolated from human, mostly from urine and blood. The decreased aggregation of these mutants could have important clinical implications as we hypothesize that such clones could better disperse within the host allowing colonisation of other body sites and potentially leading to systemic infections.

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Multidrug-Resistant Klebsiella variicola Isolated in the Urine of Healthy Bovine Heifers, a Potential Risk as an Emerging Human Pathogen

Abstract: Klebsiella variicola is an opportunistic pathogen in humans. It also has been associated with bovine mastitis, which can have significant economic effects.

Cited by 4 publications

References 59 publications

Emergence of OXA-484-Producing Klebsiella variicola in China

Emergence of OXA-484-Producing Klebsiella variicola in China

Complete genome sequence of Klebsiella variicola subsp. variicola ML.9ba2, an endophytic strain isolated from aerial roots of Philodendron erubescens

Emergence of novel non-aggregative variants under negative frequency-dependent selection in Klebsiella variicola

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