Multidrug resistance operon emrAB contributes for chromate and ampicillin co-resistance in a Staphylococcus strain isolated from refinery polluted river bank

He, Zhong; Ma, Yantian; Liu, Pu; Li, Xiangkai

doi:10.1186/s40064-016-3253-7

Cited by 16 publications

(16 citation statements)

References 51 publications

(55 reference statements)

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“…400,401 Further, the multidrug efflux system EmrAB from S. aureus has been shown to act upon chromium(VI). 402 Overall, these observations emphasize that the promiscuity of efflux systems can include the ability to translocate substrates that are very different in steric bulk and potentially charge distribution. They also support a role for metal ions in maintaining the expression of an efflux system even in the absence of its antimicrobial substrate(s).…”

Section: Resistance To Heavy Metalsmentioning

confidence: 92%

Physiological Functions of Bacterial “Multidrug” Efflux Pumps

et al. 2021

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Bacterial multidrug efflux pumps have come to prominence in human and veterinary pathogenesis because they help bacteria protect themselves against the antimicrobials used to overcome their infections. However, it is increasingly realized that many, probably most, such pumps have physiological roles that are distinct from protection of bacteria against antimicrobials administered by humans. Here we undertake a broad survey of the proteins involved, allied to detailed examples of their evolution, energetics, structures, chemical recognition, and molecular mechanisms, together with the experimental strategies that enable rapid and economical progress in understanding their true physiological roles. Once these roles are established, the knowledge can be harnessed to design more effective drugs, improve existing microbial production of drugs for clinical practice and of feedstocks for commercial exploitation, and even develop more sustainable biological processes that avoid, for example, utilization of petroleum.

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Section: Resistance To Heavy Metalsmentioning

confidence: 92%

Physiological Functions of Bacterial “Multidrug” Efflux Pumps

et al. 2021

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“…A total of 10 rRNA, 61 tRNA, and 2,510 protein-coding genes were predicted by in silico annotation of the genome. Of note, the emr A and emr B genes, implicated in chromate and ampicillin co-resistance in Staphylococcus aureus LZ 01 ( Zhang et al 2016 ), were identified in the genome of SC5. These genes are also observed in the genomes of the other four S. arlettae chromium-resistant strains isolated from the same environment.…”

Section: Resultsmentioning

confidence: 99%

Comparative Genomics Suggests a Taxonomic Revision of the Staphylococcus cohnii Species Complex

Lavecchia

Chiara

Virgilio

et al. 2021

Genome Biology and Evolution

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Staphylococcus cohnii (SC), a coagulase-negative bacterium, was first isolated in 1975 from human skin. Early phenotypic analyses led to the delineation of two subspecies (subsp.), Staphylococcus cohnii subsp. cohnii (SCC) and Staphylococcus cohnii subsp. urealyticus (SCU). SCC was considered to be specific to humans whereas SCU apparently demonstrated a wider host range, from lower primates to humans. The type strains ATCC 29974 and ATCC 49330 have been designated for SCC and SCU, respectively. Comparative analysis of 66 complete genome sequences—including a novel SC isolate—revealed unexpected patterns within the SC complex, both in terms of genomic sequence identity and gene content, highlighting the presence of 3 phylogenetically distinct groups. Based on our observations, and on the current guidelines for taxonomic classification for bacterial species, we propose a revision of the SC species complex. We suggest that SCC and SCU should be regarded as two distinct species: SC and SU (Staphylococcus urealyticus), and that two distinct subspecies, SCC and SCB (SC subsp. barensis, represented by the novel strain isolated in Bari) should be recognized within SC. Furthermore, since large scale comparative genomics studies recurrently suggest inconsistencies or conflicts in taxonomic assignments of bacterial species, we believe that the approach proposed here might be considered for more general application.

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“…Further, evidence of shared genetic tolerance mechanisms for metals and antibiotics was found in Staphylococcus aureus isolated from a polluted riverbank. S. aureus contained a novel emrAB operon encoding efflux pumps that were inducible by the heavy metals Cr(VI) and manganese and the antibiotics ampicillin and chloramphenicol (Zhang et al, 2016).…”

Section: Resultsmentioning

confidence: 99%

Comparative genomics of 16Microbacteriumspp. that tolerate multiple heavy metals and antibiotics

Learman¹,

Ahmad²,

Brookshier³

et al. 2019

PeerJ

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A total of 16 different strains of Microbacterium spp. were isolated from contaminated soil and enriched on the carcinogen, hexavalent chromium [Cr(VI)]. The majority of the isolates (11 of the 16) were able to tolerate concentrations (0.1 mM) of cobalt, cadmium, and nickel, in addition to Cr(VI) (0.5–20 mM). Interestingly, these bacteria were also able to tolerate three different antibiotics (ranges: ampicillin 0–16 μg ml−1, chloramphenicol 0–24 μg ml−1, and vancomycin 0–24 μg ml−1). To gain genetic insight into these tolerance pathways, the genomes of these isolates were assembled and annotated. The genomes of these isolates not only have some shared genes (core genome) but also have a large amount of variability. The genomes also contained an annotated Cr(VI) reductase (chrR) that could be related to Cr(VI) reduction. Further, various heavy metal tolerance (e.g., Co/Zn/Cd efflux system) and antibiotic resistance genes were identified, which provide insight into the isolates’ ability to tolerate metals and antibiotics. Overall, these isolates showed a wide range of tolerances to heavy metals and antibiotics and genetic diversity, which was likely required of this population to thrive in a contaminated environment.

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Multidrug resistance operon emrAB contributes for chromate and ampicillin co-resistance in a Staphylococcus strain isolated from refinery polluted river bank

Cited by 16 publications

References 51 publications

Physiological Functions of Bacterial “Multidrug” Efflux Pumps

Physiological Functions of Bacterial “Multidrug” Efflux Pumps

Comparative Genomics Suggests a Taxonomic Revision of the Staphylococcus cohnii Species Complex

Comparative genomics of 16Microbacteriumspp. that tolerate multiple heavy metals and antibiotics

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