Multidrug resistance in hydrocarbon-tolerant Gram-positive and Gram-negative bacteria

Stancu, Mihaela Marilena; Grifoll, Magdalena

doi:10.2323/jgam.57.1

Cited by 33 publications

(14 citation statements)

References 69 publications

(101 reference statements)

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“…In the first case, the contribution of more resistant species should increase after the perturbation. Gram-negative bacteria are generally more resistant to toxic organic compounds, such as organic solvents or antibiotics, which may be explained by their different structure resulting in the presence of the efflux pump systems and outer cell membranes (Vermuë et al 1993;Heipieper et al 2007;Heipieper and Martinez 2010;Stancu and Grifoll, 2011). As observed, the contribution of two Gram-negative genera -Pseudomonas and Sphingomonas -increased in all samples spiked with ILs, which may support the first hypothesis regarding the toxicity.…”

Section: Explaining Changes In Community Structure and Decrease In Bisupporting

confidence: 65%

Biodiversity of soil bacteria exposed to sub-lethal concentrations of phosphonium-based ionic liquids: Effects of toxicity and biodegradation

Sydow

Owsianiak

Framski

et al. 2018

Ecotoxicology and Environmental Safety

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Little is known about the effect of ionic liquids (ILs) on the structure of soil microbial communities and resulting biodiversity. Therefore, we studied the influence of six trihexyl(tetradecyl)phosphonium ILs (with either bromide or various organic anions) at sublethal concentrations on the structure of microbial community present in an urban park soil in 100-day microcosm experiments. The biodiversity decreased in all samples (Shannon's index decreased from 1.75 down to 0.74 and OTU's number decreased from 1399 down to 965) with the largest decrease observed in the microcosms spiked with ILs where biodegradation extent was higher than 80%. (i.e. [P][Br] and [P][2,4,4]). Despite this general decrease in biodiversity, which can be explained by ecotoxic effect of the ILs, the microbial community in the microcosms was enriched with Gram-negative hydrocarbon-degrading genera e.g. Sphingomonas. It is hypothesized that, in addition to toxicity, the observed decrease in biodiversity and change in the microbial community structure may be explained by the primary biodegradation of the ILs or their metabolites by the mentioned genera, which outcompeted other microorganisms unable to degrade ILs or their metabolites. Thus, the introduction of phosphonium-based ILs into soils at sub-lethal concentrations may result not only in a decrease in biodiversity due to toxic effects, but also in enrichment with ILs-degrading bacteria.

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Section: Explaining Changes In Community Structure and Decrease In Bisupporting

confidence: 65%

Biodiversity of soil bacteria exposed to sub-lethal concentrations of phosphonium-based ionic liquids: Effects of toxicity and biodegradation

Sydow

Owsianiak

Framski

et al. 2018

Ecotoxicology and Environmental Safety

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“…In agreement with this we propose that certain bacteria may benefit from these broad range transformations during detoxification of hydrocarbons. The strong toxic effects of hydrocarbons and other nonpolar solvents on microorganisms and the mechanisms involved in solvent tolerance of bacteria have been investigated intensively (for further information see Sikkema et al, 1995 ; Kobayashi et al, 2000 ; Trautwein et al, 2008 ; Zink and Rabus, 2010 ; Stancu and Grifoll, 2011 ; Baumgarten et al, 2012 ; Segura et al, 2012 ; Alvarez-Ortega et al, 2013 ).…”

Section: Discussionmentioning

confidence: 99%

Versatile transformations of hydrocarbons in anaerobic bacteria: substrate ranges and regio- and stereo-chemistry of activation reactions†

et al. 2015

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Anaerobic metabolism of hydrocarbons proceeds either via addition to fumarate or by hydroxylation in various microorganisms, e.g., sulfate-reducing or denitrifying bacteria, which are specialized in utilizing n-alkanes or alkylbenzenes as growth substrates. General pathways for carbon assimilation and energy gain have been elucidated for a limited number of possible substrates. In this work the metabolic activity of 11 bacterial strains during anaerobic growth with crude oil was investigated and compared with the metabolite patterns appearing during anaerobic growth with more than 40 different hydrocarbons supplied as binary mixtures. We show that the range of co-metabolically formed alkyl- and arylalkyl-succinates is much broader in n-alkane than in alkylbenzene utilizers. The structures and stereochemistry of these products are resolved. Furthermore, we demonstrate that anaerobic hydroxylation of alkylbenzenes does not only occur in denitrifiers but also in sulfate reducers. We propose that these processes play a role in detoxification under conditions of solvent stress. The thermophilic sulfate-reducing strain TD3 is shown to produce n-alkylsuccinates, which are suggested not to derive from terminal activation of n-alkanes, but rather to represent intermediates of a metabolic pathway short-cutting fumarate regeneration by reverse action of succinate synthase. The outcomes of this study provide a basis for geochemically tracing such processes in natural habitats and contribute to an improved understanding of microbial activity in hydrocarbon-rich anoxic environments.

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“…Overnight culture broths of S. marcescens IBBPo15 cells were inoculated on liquid LB-Mg (20 mM MgSO4) medium (M.M. STANCU & M. GRIFOLL [6]) in the presence of 5% organic solvents (alkanes: cyclohexane, n-hexane, n-decane, aromatics: toluene, styrene, ethylbenzene). Flasks were sealed and incubated for 24 hours at 37°C on a rotary shaker (200 rpm).…”

Section: Methodsmentioning

confidence: 99%

Effect of high growth temperature on Serratia marcescens

Stancu¹

2020

Rom Biotechnol Lett.

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The purpose of this study was to investigate some parameters of S. marcescens IBBPo15 cells adaptation to toxic organic solvents. S. marcescens IBBPo15 cells incubated at 37°C exhibited a slower growth when they were exposed to 5% organic solvents (alkanes, aromatics), as compared with the control cells. S. marcescens IBBPo15 cells grown at 37°C produced protease and the enzyme production decreased in cells exposed to aromatic compounds, as compared with the control cells. Surfactant serrawettin and red pigment prodigiosin were produced by S. marcescens IBBPo15 cells grown at 37°C. Modifications in the pigment and extracellular proteins profile were observed in S. marcescens IBBPo15 cells grown at 37°C during exposure to organic solvents (alkanes, aromatics). Alkane hydroxylase (i.e., alkB1) and toluene dioxygenase (i.e., todM) catabolic genes, 4'-phosphopantetheinyl transferase (i.e., pswP) and serralysin metalloproteinase (i.e., ser) genes were detected by polymerase chain reaction in DNA extracted from S. marcescens IBBPo15 control cells grown at 37°C, as well as in cells exposed to organic solvents (excepting todM in n-decane exposed cells).

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Multidrug resistance in hydrocarbon-tolerant Gram-positive and Gram-negative bacteria

Cited by 33 publications

References 69 publications

Biodiversity of soil bacteria exposed to sub-lethal concentrations of phosphonium-based ionic liquids: Effects of toxicity and biodegradation

Biodiversity of soil bacteria exposed to sub-lethal concentrations of phosphonium-based ionic liquids: Effects of toxicity and biodegradation

Versatile transformations of hydrocarbons in anaerobic bacteria: substrate ranges and regio- and stereo-chemistry of activation reactions†

Effect of high growth temperature on Serratia marcescens

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