Multidrug resistance and prevalence of quinolone resistance genes of Salmonella enterica serotypes 4,[5],12:i:- in China

He, Jun; Sun, Fan; Sun, Dewei; Wang, Zhenyu; Jin, Shanshan; Pan, Zhiming; Xu, Zhengzhong; Chen, Xiang; Jiao, Xinan

doi:10.1016/j.ijfoodmicro.2020.108692

Cited by 25 publications

(19 citation statements)

References 30 publications

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“…Isolated Salmonella serovars showing intermediate resistance to quinolones, particularly fluoroquinolones, were presented in Table 1 . In the last two decades, Salmonella 4,[5],12:i:- has rapidly emerged worldwide [ 14 ]. In South Korea, the first foodborne outbreak of Salmonella 4,[5],12:i:- was reported in 2008 [ 20 ]; the same serovar, which exhibits nalidixic acid resistance, was isolated from pigs and chickens in South Korea [ 21 ].…”

Section: Resultsmentioning

confidence: 99%

“…In South Korea, the first foodborne outbreak of Salmonella 4,[5],12:i:- was reported in 2008 [ 20 ]; the same serovar, which exhibits nalidixic acid resistance, was isolated from pigs and chickens in South Korea [ 21 ]. Similarly, most Salmonella 4,[5],12:i:- isolates from chickens, geese, and cats in China were resistant to nalidixic acid (52.5%) [ 14 ]. Isolates of enrofloxacin-resistant Salmonella 4,[5],12:i:- from swine in the United States have also been reported [ 22 ].…”

Section: Resultsmentioning

confidence: 99%

“…In Canada, a relatively high prevalence of PMQR genes has been reported in human isolates of non-typhoidal Salmonella with resistance and reduced susceptibility to fluoroquinolones [ 13 ]. Likewise, PMQR gene-positive Salmonella 4,[5],12:i:- were recently isolated from pigs, chickens, humans, geese, and cats in China [ 14 ]. In this study, we aim to estimate the presence of the plasmid-mediated quinolone resistance genes and their association with fluoroquinolone susceptibility in non-typhoidal Salmonella isolates from human clinical samples in South Korea from 2016 to 2019.…”

Section: Introductionmentioning

confidence: 99%

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Presence of plasmid-mediated quinolone resistance (PMQR) genes in non-typhoidal Salmonella strains with reduced susceptibility to fluoroquinolones isolated from human salmonellosis in Gyeonggi-do, South Korea from 2016 to 2019

et al. 2021

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Non-typhoidal salmonellosis remains a pressing public health problem worldwide. Quinolones, particularly fluoroquinolones, are widely used to treat various infections, including non-typhoidal salmonellosis, which can be a serious illness. The emergence of fluoroquinolone-resistant Salmonella has resulted in treatment failure and high mortality rates. In this study, we estimated the presence of plasmid-mediated quinolone resistance (PMQR) genes in Salmonella enterica isolated from human salmonellosis patients in South Korea from 2016 to 2019. We evaluated the association of these genes with fluoroquinolone susceptibility. Antimicrobial susceptibility tests for Salmonella isolates were performed using the Vitek II system, and the minimum inhibitory concentrations (MIC) of ciprofloxacin and levofloxacin were determined using the E-test method. Plasmid-mediated quinolone resistance (PMQR) genes were detected by PCR amplification and quinolone resistance-determining regions (QRDRs) of the gyrA and parC genes were analyzed following Sanger sequencing of the PCR products. Thirty-four Salmonella strains with reduced susceptibility to fluoroquinolones (ciprofloxacin MIC ≥ 0.125 µg/mL and levofloxacin MIC ≥ 0.25 µg/mL) were selected from 208 human clinical Salmonella isolates. Among them, 22 Salmonella strains harbored one PMQR gene (qnrA, qnrB, or qnrS), and three Salmonella strains carried two PMQR genes (qnrS and aac(6′)-Ib-cr or qnrA and qnrB). qnrS was the most common PMQR gene. Serotyping revealed that Salmonella 4,[5]12:i:- (32.4%, 11/34) and Salmonella Typhimurium (29.4%, 10/34) were the two most predominant serovars, and Multi-locus sequence typing (MLST) showed that ST19 and ST34 were the most frequent sequence types. In conclusion, qnr gene-positive Salmonella 4,[5],12:i:- and Salmonella Typhimurium were the main serovars responsible for reduced susceptibility to fluoroquinolones. Therefore, our findings suggest that PMQR-positive Salmonella strains, which can be isolated from various samples including human, food, and the environment, should be carefully monitored.

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Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Presence of plasmid-mediated quinolone resistance (PMQR) genes in non-typhoidal Salmonella strains with reduced susceptibility to fluoroquinolones isolated from human salmonellosis in Gyeonggi-do, South Korea from 2016 to 2019

et al. 2021

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“…Isolates showed a high-level MDR (89.6%) in our study, which was much higher than that of previous studies in China [ 34 ], Switzerland [ 42 ], and Denmark [ 35 ]. Over the last two decades, two primary MDR clones of Salmonella 4,[5],12:i:- were recognized as important for public health [ 43 ]. One clonal line (European clone) was characterized by chromosomally encoded resistance to ampicillin, streptomycin, sulfonamides, and tetracyclines (ASSuT), and another clonal line (Spanish clone) was characterized by plasmid-encoded resistance to ampicillin, chloramphenicol, sulfonamides, gentamicin, streptomycin, tetracycline, and trimethoprim (ACSuGSTTm) [ 10 , 43 ].…”

Section: Discussionmentioning

confidence: 99%

“…Over the last two decades, two primary MDR clones of Salmonella 4,[5],12:i:- were recognized as important for public health [ 43 ]. One clonal line (European clone) was characterized by chromosomally encoded resistance to ampicillin, streptomycin, sulfonamides, and tetracyclines (ASSuT), and another clonal line (Spanish clone) was characterized by plasmid-encoded resistance to ampicillin, chloramphenicol, sulfonamides, gentamicin, streptomycin, tetracycline, and trimethoprim (ACSuGSTTm) [ 10 , 43 ]. In our study, 44.8% and 12.6% isolates displayed similar MDR profiles with European and Spanish clones, respectively.…”

Section: Discussionmentioning

confidence: 99%

Highly prevalent MDR, frequently carrying virulence genes and antimicrobial resistance genes in Salmonella enterica serovar 4,[5],12:i:- isolates from Guizhou Province, China

Long

Wang

et al. 2022

PLoS ONE

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Salmonella enterica serovar 4,[5],12:i:-, a monophasic variant of Salmonella Typhimurium lacking the phase 2 flagellin, is one of the common serotypes causing Salmonellosis worldwide. However, information on Salmonella serovar 4,[5],12:i:- from Guizhou Province has lacked so far. This study aimed to investigate the antimicrobial resistance, the presence of antimicrobial resistance genes and virulence genes, and characterize the MLST genotypes of Salmonella serovar 4,[5],12:i:- isolates from Guizhou province, China. We collected 363 non-typhoid Salmonella (NTS) isolates of Guizhou from 2013 to 2018. Biochemical identification, serogroups testing, and specific multiplex polymerase chain reaction (mPCR) assay were conducted to identify Salmonella 4,[5],12:i:- isolates. Isolates were determined the antimicrobial resistance by the micro broth dilution method, detected the presence of antimicrobial resistance genes and virulence genes by PCR, and examined the molecular genotyping by Multilocus sequence typing (MLST). Eighty-seven Salmonella 4,[5],12:i:- isolates were detected, accounting for 23.9% (87/363) of the total NTS isolates. All Salmonella 4,[5],12:i:- isolates showed highly resistant to sulfaoxazole (93.1%), streptomycin (90.8%), ampicillin (88.5%), tetracycline (86.2%) and doxycycline (86.2%). A high proportion (94.2%) of multi-drug resistance (MDR) isolates were found. Most (83.9%) Salmonella 4,[5],12:i:- isolates carried four antimicrobial resistance genes, especially blaTEM-1, strA-strB, sul2, and tetB genes. Salmonella 4,[5],12:i:- isolates showed a high rate of invA, sseL, mgtC, siiE, sopB, gipA, gtgB, sspH1, and sspH2 (72.4%~98.9%). On the contrary, none of the isolates were detected the spvC and pefA genes. MLST analysis revealed three sequence types (STs), and ST34 (97.7%) was the dominant sequence type. This study is the first report of Salmonella 4,[5],12:i:- in humans from Guizhou province, China. The data might be useful for rational antimicrobial usage against Salmonella 4,[5],12:i:- infections, risk management, and public health strategies in Guizhou.

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Genomic Epidemiology of ST34 Monophasic Salmonella enterica Serovar Typhimurium from Clinical Patients from 2008 to 2017 in Henan, China

Mu¹,

Li²,

Du³

et al. 2022

Engineering

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Multidrug resistance and prevalence of quinolone resistance genes of Salmonella enterica serotypes 4,[5],12:i:- in China

Cited by 25 publications

References 30 publications

Presence of plasmid-mediated quinolone resistance (PMQR) genes in non-typhoidal Salmonella strains with reduced susceptibility to fluoroquinolones isolated from human salmonellosis in Gyeonggi-do, South Korea from 2016 to 2019

Presence of plasmid-mediated quinolone resistance (PMQR) genes in non-typhoidal Salmonella strains with reduced susceptibility to fluoroquinolones isolated from human salmonellosis in Gyeonggi-do, South Korea from 2016 to 2019

Highly prevalent MDR, frequently carrying virulence genes and antimicrobial resistance genes in Salmonella enterica serovar 4,[5],12:i:- isolates from Guizhou Province, China

Genomic Epidemiology of ST34 Monophasic Salmonella enterica Serovar Typhimurium from Clinical Patients from 2008 to 2017 in Henan, China

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