Multidrug resistance and ESBL-producing Salmonella spp. isolated from broiler processing plants

Ziech, Rosângela Estel; Lampugnani, Camila; Perin, Ana Paula; Sereno, Mallú Jagnow; Sfaciotte, Ricardo Antonio Pilegi; Viana, Cibeli; Soares, Vanessa Mendonça; Pinto, José Paes de Almeida Nogueira; Bersot, Luciano dos Santos

doi:10.1016/j.bjm.2015.11.021

Cited by 42 publications

(26 citation statements)

References 33 publications

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“…This result is in agreement with those obtained by other authors, which found reduced efficacy of this antimicrobial against salmonellae, maybe because of the indiscriminate use in humans and animals therapy [40][41][42].…”

Section: Discussionsupporting

confidence: 93%

Salmonella infection in healthy pet reptiles: Bacteriological isolation and study of some pathogenic characters

Bertelloni¹,

Chemaly²,

Cerri³

et al. 2016

Acta Microbiologica et Immunologica Hungarica

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The fecal samples from 213 captive reptiles were examined, and 29 (13.61%) Salmonella enterica isolates were detected: 14/62 (22.58%) from chelonians, 14/135 (10.37%) from saurians, and 1/16 (6.25%) from ophidians. The isolates were distributed among 14 different serotypes: Miami, Ebrie, Hermannsweder, Tiergarten, Tornov, Pomona, Poona, Goteborg, Abaetetube, Nyanza, Kumasi, Typhimurium, 50:b:z 6 , 9,12:z 29 :1,5, and a non-motile serotype with antigenic formula 1,4,[5],12:-:-. Salmonella typhimurium and 50:b:z 6 isolates showed the spv plasmid virulence genes, responsible of the capability to induce extra-intestinal infections. In some cases, pulsed field gel electrophoresis revealed different profiles for the strains of the same serotypes, showing different origins, whereas a common source of infection was supposed when one pulsotype had been observed for isolates of a serovar. Twentyseven (93.10%) isolates showed resistance to one or more antibiotics. Ceftazidime was active to all the tested isolates, whereas the highest percentages of strains were no susceptible to tigecycline (93.10%), streptomycin (89.66%), and sulfonamide (86.21%).

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Section: Discussionsupporting

confidence: 93%

Salmonella infection in healthy pet reptiles: Bacteriological isolation and study of some pathogenic characters

Bertelloni¹,

Chemaly²,

Cerri³

et al. 2016

Acta Microbiologica et Immunologica Hungarica

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“…In the present study, the most frequent profile of the isolates was weak biofilm formation and resistance to quinolones, sulfonamides, tetracyclines, cephalosporin, and penicillins (Figure 1). On the other hand, Ziech et al (2016b), evaluating Salmonella sp. strains isolated in broiler processing plants, detected 86% multiresistant strains, and the most frequent profile was weak biofilm formation, production of wide-spectrumbetalactamase, and resistance to beta-lactams, quinolones, and tetracyclines.…”

Section: Resultsmentioning

confidence: 99%

Antimicrobial Susceptibility and Biofilm Production by Salmonella sp. Strains Isolated from Frozen Poultry Carcasses

Sereno

Ziech

Druziani

et al. 2017

Rev. Bras. Cienc. Avic.

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The objectives of this study were to evaluate the antimicrobial resistance and the biofilm-producing ability of Salmonella sp. strains isolated from frozen poultry carcasses. Antimicrobial susceptibility was tested by the disk-diffusion method. Biofilm-producing ability was determined in 96-well polystyrene microplates stained with crystal violet at 1%. Out of the 22 strains tested, all were multiresistant, that is, resistant to more than three antimicrobial classes, and 72.7% were able to form biofilms. The highest resistance rates obtained were against sulfonamides, tetracycline, and quinolones. On the other hand, 100% of the strains were sensitive to chloramphenicol. According to the rate of biofilm formation, 3 (13.6%) and 13 (59.1%) strains were classified as moderate and weak biofilm-producers, respectively, and 27.3% did not form biofilms. Biofilms increase the tolerance of microorganisms to stress, reducing their sensitivity to disinfectants and antimicrobials; favor equipment corrosion; and act as substrates for the adhesion of bacteria with lower biofilm-producing capacity. The results of the present study stress the importance of cleaning procedures in food processing plants and highlight the public health risks related to the emergence of multiresistant strains.

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“…However, the widespread use and even abuse of antibiotics in animal husbandry have facilitated the emergence and dissemination of antibiotic resistance in Salmonella , which has posed a serious challenge for the health of animals and humans ( Marshall and Levy, 2011 ; Mukerji et al, 2017 ). Noticeably, numerous studies in recent years have indicated that extended-spectrum β-lactamase (ESBL)-producing Salmonella has been frequently isolated from food-producing animals and animal-derived foods in many countries of the world, including China ( Wu et al, 2013 ; Chon et al, 2015 ; Franco et al, 2015 ; Hu et al, 2015 ; Noda et al, 2015 ; Ziech et al, 2016 ; Zhao et al, 2017 ). ESBL-producing Salmonella is able to inactivate and hydrolyze the β-lactam ring in β-lactam antibiotics and third- and fourth-generation cephalosporins, leading to the increase of treatment cost and even to therapy failure, which has triggered a serious public concern ( Bonnet, 2004 ; Pitout and Laupland, 2008 ).…”

Section: Introductionmentioning

confidence: 99%

Prevalence and Antibiotic Resistance of Non-typhoidal Salmonella Isolated from Raw Chicken Carcasses of Commercial Broilers and Spent Hens in Tai’an, China

Zhou²,

Miao

2017

Front. Microbiol.

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The present study was aimed to determine the prevalence and characteristics of Salmonella isolated from meat samples of commercial broilers (CB) and spent hens (SH). Between March and June 2016, 200 retail raw chicken carcasses (100 from CB and 100 from SH) were obtained from local supermarkets in Tai’an city of China, and Salmonella isolates were then analyzed for antibiotic resistance, serotype, β-lactamase genes, and the presence of class 1 integron. Forty Salmonella strains were obtained in this study (CB: 21/100, 21%; SH: 19/100, 19%). Three serotypes were identified in 40 Salmonella, and S. Enteritidis (CB: 15/21, 71.4%; SH: 10/19, 52.6%) was the dominant serotype, followed by S. Typhimurium (CB: 4/21, 19%; SH: 6/19, 31.6%) and S. Derby (CB: 2/21, 9.5%; SH: 3/19, 15.8%). Among 21 Salmonella isolated from CB, high antibiotic resistance rates were found for ampicillin (20/21, 95.2%), nalidixic acid (18/21, 85.7%), cefotaxime (17/21, 81%), and tetracycline (13/21, 61.9%); class 1 integron was observed in seven isolates (7/21, 33.3%), and gene cassettes included an empty integron (0.15 kb, n = 1), aadA2 (1.2 kb, n = 3), drfA1-aadA1 (1.4 kb, n = 1), and drfA17-aadA5 (1.7 kb, n = 2); blaTEM-1 was the dominant β-lactamase gene (21/21, 100%), followed by blaCTX-M-55 (7/21, 33.3%). Among 19 Salmonella isolated from SH, high antibiotic resistance rates were found for nalidixic acid (19/19, 100%), tetracycline (19/19, 100%), ampicillin (18/19, 94.7%), and ciprofloxacin (13/19, 68.4%); class 1 integron was observed in two isolates (2/19, 10.5%), and gene cassettes included drfA17-aadA5 (1.7 kb, n = 1) and drfA1-aadA1 (1.4 kb, n = 1); blaTEM-1 was the dominant β-lactamase gene (19/19, 100%), followed by blaCTX-M-55 (2/19, 10.5%) and blaCMY-2 (1/19, 5.3%). Collectively, antibiotic-resistant Salmonella can be widely detected in retail raw chicken carcasses of CB and SH, and therefore can pose a serious risk to public health.

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Multidrug resistance and ESBL-producing Salmonella spp. isolated from broiler processing plants

Cited by 42 publications

References 33 publications

Salmonella infection in healthy pet reptiles: Bacteriological isolation and study of some pathogenic characters

Salmonella infection in healthy pet reptiles: Bacteriological isolation and study of some pathogenic characters

Antimicrobial Susceptibility and Biofilm Production by Salmonella sp. Strains Isolated from Frozen Poultry Carcasses

Prevalence and Antibiotic Resistance of Non-typhoidal Salmonella Isolated from Raw Chicken Carcasses of Commercial Broilers and Spent Hens in Tai’an, China

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