Multidrug Resistance-1 in T Lymphocytes and Natural Killer Cells of Adults with Idiopathic Thrombocytopenic Purpura: Effect of Prednisone Treatment

“…These results suggested that CD8 + T cells might contain the main target sites of P-gp in ITP. Given the differences of P-gp functional activity in lymphocyte subsets, the different target cells evaluated in separate studies might also lead to the discrepancies in results [25][26][27].…”

Section: Discussionmentioning

confidence: 86%

“…The majority of previous studies investigated the potential differences between patients with ITP and healthy controls only. However, considering the variance of P-gp function within ITP patients, we think the discrepancies in patient samples would lead to inconsistent results in different studies [25][26][27].…”

Section: Discussionmentioning

confidence: 95%

“…Ruiz-Soto and Levy [25,26] reported increased P-gp activity in ITP and Evans syndrome patients, respectively. Whereas, Lopez-Karpovitch [27] found that T and NK cells did not show increased MDR-1 functional activity in patients with ITP. The majority of previous studies investigated the potential differences between patients with ITP and healthy controls only.…”

Section: Discussionmentioning

confidence: 95%

“…MDR-1 gene polymorphisms and P-gp efflux in patients with ITP have been investigated by several studies [23][24][25][26][27], while the expression and efflux function of P-gp were unascertained in patients with ITP. Furthermore, the immunemodulation functions of P-gp in ITP remain unknown.…”

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Effects of the multidrug resistance-1 gene on drug resistance in primary immune thrombocytopenia

Liu

Zhang

et al. 2016

Autoimmunity

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Primary immune thrombocytopenia (ITP) is an autoimmune disorder that is characterized by low platelet count. Glucocorticoids (GCs) resistance is a great challenge in the treatment of ITP. P-glycoprotein (P-gp) is a widely studied protein, which is associated with drug resistance. However, in ITP, the functional activity and immune regulation mechanism of P-gp remain uncertain. In this study, we evaluated the expression and functional activity of P-gp in different lymphocyte subsets, explored the correlation between P-gp function and GCs resistance and investigated the role of P-gp in ITP pathogenesis. Results indicated that the functional activity and mRNA level of P-gp were significantly higher in GCs-nonresponsive patients than in GCs-responsive patients with ITP. However, these differences in P-gp were only significant in CD8 T cells. P-gp function was related to disease activity rather than GCs therapy. P-gp was involved in secreting granzyme B and perforin, maintaining autoreactive lymphocytes survival and enhancing autologous platelets lysis in ITP. In conclusion, over-functional P-gp might play an important role in the pathogenesis of ITP and induce GCs resistance in nonresponsive ITP patients. The blockage of P-gp could be a promising therapeutic approach for GCs-resistant patients with ITP.

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Immune Thrombocytopenia

Sandhu

2017

Hospital Medicine Clinics

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The objective: This study was undertaken to detect characterization of the different gene polymorphisms in Human killer cell immunoglobulin-like receptor (KIR2) gene and multi-drug resistance (MDR1) gene, among childhood ITP Egyptian patients. In addition to assess the potential role of these polymorphisms in relation to types of ITP and response to different treatment modalities. Patients and Methods: A total of 48 pediatric patients with immune thrombocytopenia (ITP; 24 newly diagnosed and 24 chronic) and 35 healthy controls were investigated via polymerase chain reaction-restriction fragment length polymorphism analysis for multidrug resistance (MDR) 1 and killer cell immunoglobulin-like receptor (KIR) 2 genes. Results: The frequency of MDR1 gene in patients and control was not significant (P ¼ .090). The CT genotype was the highest distribution among all ITP cases (62.50%, n ¼ 30) and control (48.60%, n ¼ 17). There was a significant difference in age at diagnosis of MDR1 gene with the CC genotype had the eldest age and lowest initial platelets count (P ¼ .029 and P ¼ .004). The distribution of KIR2 gene among all patients with ITP and controls was significant (P ¼ .026) with (KIRDL2À/KIRDS2À) genotype was the most prevalent among patients. Conclusion: The frequency of MDR1 polymorphisms was not associated with susceptibility to the development and clinical progression of the disease. However, KIR2 gene polymorphisms were independently associated with childhood ITP in Egyptian patients with highest prevalence among (KIRDL2À/KIRDS2À) genotypes.

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Multidrug Resistance-1 in T Lymphocytes and Natural Killer Cells of Adults with Idiopathic Thrombocytopenic Purpura: Effect of Prednisone Treatment

Cited by 8 publications

References 20 publications

Relation between 3435C>T multidrug resistance 1 gene polymorphism with high dose methylprednisolone treatment of childhood acute idiopathic thrombocytopenic purpura

Relation between 3435C>T multidrug resistance 1 gene polymorphism with high dose methylprednisolone treatment of childhood acute idiopathic thrombocytopenic purpura

Effects of the multidrug resistance-1 gene on drug resistance in primary immune thrombocytopenia

Immune Thrombocytopenia

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