Multidrug- and colistin-resistant Salmonella enterica 4,[5],12:i:- sequence type 34 carrying the mcr-3.1 gene on the IncHI2 plasmid recovered from a human

Monte, Daniel Farias Marinho do; Nelson, Valerie; Cerdeira, Louise; Keelara, Shivaramu; Greene, Shermalyn R.; Griffin, Denise; Rath, Shadia; Hall, Robbie; Page, Nichole; Fedorka–Cray, Paula J.; Thakur, Siddhartha

doi:10.1099/jmm.0.001012

Cited by 23 publications

(27 citation statements)

References 17 publications

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“…Evidence shows that the IncX4 plasmids harbouring mcr-1 genes have been recovered from human and animal E. coli isolates however, our study recovered these plasmids from the poultry environment [43]. Another study conducted in China also detected PMCR genes -mcr 1 in E. coli isolates sourced from the aquatic environment [44] however, the mcr 3.1 gene was detected in a human Salmonella case in the US [45]. This further buttresses that mcr gene has spread across multiple pathogens.…”

Section: Discussionsupporting

confidence: 57%

Genetic relatedness of multidrug resistant Escherichia coli isolated from humans, chickens and poultry environments

Aworh

Kwaga

Hendriksen

et al. 2020

Preprint

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Background: Inappropriate use of antimicrobial agents in animal production has led to the development of antimicrobial resistance (AMR) in foodborne pathogens. Transmission of AMR foodborne pathogens from reservoirs, particularly chickens to the human population do occur. Recently, we reported that occupational exposure was a risk factor for multidrug-resistant (MDR) Escherichia coli (E. coli) among poultry-workers. Here we determined the prevalence and genetic relatedness among MDR E. coli isolated from poultry-workers, chickens, and poultry environments in Abuja, Nigeria. This study was conducted to address the gaps identified by the Nigerian AMR situation analysis.Methods: We conducted a cross-sectional study among poultry-workers, chickens, and poultry farm/live bird market (LBM) environments. The isolates were tested phenotypically for their antimicrobial susceptibility profiles, genotypically characterized using whole-genome sequencing (WGS) and in silico multilocus sequence types (MLST). We conducted a phylogenetic single nucleotide polymorphism (SNPs) analysis to determine relatedness and clonality among the isolates.Results: A total of 115 (26.8%) out of 429 samples were positive for E. coli. Of these, 110 isolates were viable for phenotypic and genotypic characterization. The selection comprised 47 (42.7%) isolates from poultry-workers, 36 (32.7%) from chickens, and 27 (24.5%) from poultry-farm or LBM environments. Overall, 101 (91.8%) isolates were determined MDR conferring resistance to at least three drug classes. High frequency of resistance were observed for tetracycline (n = 102; 92.7%), trimethoprim/sulfamethoxazole (n = 93; 84.5%), streptomycin (n = 87; 79.1%) and ampicillin (n = 88; 80%). Two plasmid-mediated colistin genes – mcr-1.1 harboured on IncX4 plasmids were detected in environmental isolates. The most prevalent sequence types (ST) were ST-155 (n = 8), ST-48 (n = 8) and ST-10 (n = 6). Two isolates of human and environmental sources with a SNPs difference of 6161 originating from the same farm shared a novel ST. The isolates had similar AMR genes and plasmid replicons.Conclusion: MDR E.coli isolates were prevalent amongst poultry-workers, poultry, and the poultry farm/LBM environment. The emergence of MDR E. coli with novel ST in two isolates may be plasmid-mediated. Competent authorities should enforce AMR regulations to ensure prudent use of antimicrobials to limit the risk of transmission along the food chain.

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Section: Discussionsupporting

confidence: 57%

Genetic relatedness of multidrug resistant Escherichia coli isolated from humans, chickens and poultry environments

Aworh

Kwaga

Hendriksen

et al. 2020

Preprint

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“…Recently, some studies proposed it as the global pandemic clone ( Alicia et al, 2018 ; Mulvey et al, 2018 ; Biswas et al, 2019 ). Importantly, Salmonella 4,[5],12:i:- were suggested to be associated with swine ( Linxian et al, 2017 ) and currently considered with high frequency of multidrug resistance potential among Salmonella serovars, posing significant public health concerns worldwide ( Elnekave et al, 2018 ; Mather et al, 2018 ; Monte et al, 2019 ).…”

Section: Introductionmentioning

confidence: 99%

“…As a result of the extensive use of colistin for veterinary purposes especially for the control and prophylaxis of Enterobacteriaceae infections, the mobilized colistin-resistant ( mcr ) Salmonella 4,[5],12:i:- has been disseminated in humans, animals, as well as food products, including pork ( Linxian et al, 2017 ; Alicia et al, 2018 ; Elbediwi et al, 2019 ). mcr carrying Salmonella 4,[5],12:i:- has also been reported from Asia, European countries, and North America ( Monte et al, 2019 ). However, the study of Salmonella 4,[5],12:i:- in the finishing pigs is largely lacking and the direct evidence of the pork-borne transmission, particularly in certain critical antimicrobial resistance, i.e., mobile colistin resistance gene migration, remains obscure in China.…”

Section: Introductionmentioning

confidence: 99%

Genomic Characterization of mcr-1-carrying Salmonella enterica Serovar 4,[5],12:i:- ST 34 Clone Isolated From Pigs in China

Elbediwi

Pan

et al. 2020

Front. Bioeng. Biotechnol.

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Salmonella enterica serovar 4,[5],12:i:-, so-called Typhimurium monophasic variant, has become one of the most frequently isolated serovars both in humans and in animals all over the world. The increasing prevalence of mcr-1-carrying Salmonella poses significant global health concerns. However, the potential role of Salmonella 4,[5],12:i:-in mcr-1 gene migration through the food chain to the human remains obscure. Here, we investigated 337 Salmonella isolates from apparently healthy finishing pigs, which is rarely studied, obtained from pig farms and slaughterhouses in China. The mcr-1 gene was found in four colistin-resistant S. enterica 4,[5],12:i:-isolates. Notably, all four isolates belonged to sequence type 34 (ST34) with multidrug resistance phenotype. Further genomic sequencing and antimicrobial resistance characterization confirmed that mcr was responsible for the colistin resistance, and the conjugation assay demonstrated that three of four isolates carried mcr-1 in IncHI2 plasmid. Importantly, mcr-1 and class-1 integron were found to co-localize in two strains with IncHI2 plasmid. By collecting all the mcr-1-carrying Typhimurium and monophasic variant strains across the food chain (farm animals, animal-origin food, and humans), our phylogenomic analysis of available 66 genomes, including four strains in this study, demonstrated an independent phylogenetic cluster of all eight Chinese swine-originated isolates and one human isolate. Together, this study provides direct evidence for clonal and pork-borne transmission of mcr-1 by Salmonella 4,[5],12:i:-ST34 in China and highlighted a domestication pathway by acquisition of additional antimicrobial resistance determinants in Chinese ST34 isolates.

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“…In recent years, the endemic of STM ST34 has also been reported from this region. 25,42,43 To study NTS pathogenesis, the streptomycin-pretreated mouse colitis model has been extensively used. Nonetheless, the distinct pathovariants of STM might result in the altered pathogenicity in mice.…”

Section: Discussionmentioning

confidence: 99%

Pathogenicity of clinical Salmonella enterica serovar Typhimurium isolates from Thailand in a mouse colitis model

Sarichai

Buddhasiri

Walters

et al. 2020

Microbiology and Immunology

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Salmonella enterica serovar Typhimurium (S. Typhimurium [STM]) is a leading cause of nontyphoidal salmonellosis (NTS) worldwide. The pathogenesis of NTS has been studied extensively using a streptomycinpretreated mouse colitis model with the limited numbers of laboratory STM strains. However, the pathogenicity of the clinically isolated STM (STMC) strains endemic in Thailand in mice has not been explored. The aim of this study was to compare the pathogenicity of STMC strains collected from Northern Thailand with the laboratory STM (IR715) in mice. Five STMC isolates were obtained from the stool cultures of patients with acute NTS admitted to Maharaj Nakorn Chiang Mai Hospital in 2016 and 2017. Detection of virulence genes and sequence type (ST) of the strains was performed. Female C57BL/6 mice were pretreated with streptomycin sulfate 1 day prior to oral infection with STM. On Day 4 postinfection, mice were euthanized, and tissues were collected to analyze the bacterial numbers, tissue inflammation, and cecal histopathological score. We found that all five STMC strains are ST34 and conferred the same or

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Multidrug- and colistin-resistant Salmonella enterica 4,[5],12:i:- sequence type 34 carrying the mcr-3.1 gene on the IncHI2 plasmid recovered from a human

Cited by 23 publications

References 17 publications

Genetic relatedness of multidrug resistant Escherichia coli isolated from humans, chickens and poultry environments

Genetic relatedness of multidrug resistant Escherichia coli isolated from humans, chickens and poultry environments

Genomic Characterization of mcr-1-carrying Salmonella enterica Serovar 4,[5],12:i:- ST 34 Clone Isolated From Pigs in China

Pathogenicity of clinical Salmonella enterica serovar Typhimurium isolates from Thailand in a mouse colitis model

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