Multidimensional Raman Spectroscopic Signatures as a Tool for Forensic Identification of Body Fluid Traces: A Review

Sikirzhytski, Vitali; Sikirzhytskaya, Aliaksandra; Lednev, Igor K.

doi:10.1366/11-06455

Cited by 87 publications

(68 citation statements)

References 77 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…210 The assignment of certain bands to fibrin was later revised 211 as more was learned about the effect of intense laser exposure. 201 After understanding of the chemical heterogeneity of blood spatters increased, Lednev et al proposed that multi-dimensional Raman measurements be attempted, 212 and in 2012 they used the technique to discriminate between dry samples that contained mixtures of human blood and semen. With their multivariate algorithm, mixtures with blood content higher than 80% could not be distinguished from pure blood, while 5% blood in a mixture resulted in a Raman spectrum distinguishable from the spectrum of pure semen 213 (later studies would report on the successful discrimination between dry samples of peripheral blood and menstrual blood, 214 and also between races 215 ).…”

Section: Whole Bloodmentioning

confidence: 99%

Raman Spectroscopy of Blood and Blood Components

et al. 2017

View full text Add to dashboard Cite

Blood is a bodily fluid that is vital for a number of life functions in animals. To a first approximation, blood is a mildly alkaline aqueous fluid (plasma) in which a large number of free-floating red cells (erythrocytes), white cells (leucocytes), and platelets are suspended. The primary function of blood is to transport oxygen from the lungs to all the cells of the body and move carbon dioxide in the return direction after it is produced by the cells' metabolism. Blood also carries nutrients to the cells and brings waste products to the liver and kidneys. Measured levels of oxygen, nutrients, waste, and electrolytes in blood are often used for clinical assessment of human health. Raman spectroscopy is a nondestructive analytical technique that uses the inelastic scattering of light to provide information on chemical composition, and hence has a potential role in this clinical assessment process. Raman spectroscopic probing of blood components and of whole blood has been on-going for more than four decades and has proven useful in applications ranging from the understanding of hemoglobin oxygenation, to the discrimination of cancerous cells from healthy lymphocytes, and the forensic investigation of crime scenes. In this paper, we review the literature in the field, collate the published Raman spectroscopy studies of erythrocytes, leucocytes, platelets, plasma, and whole blood, and attempt to draw general conclusions on the state of the field.

show abstract

Section: Whole Bloodmentioning

confidence: 99%

Raman Spectroscopy of Blood and Blood Components

et al. 2017

View full text Add to dashboard Cite

show abstract

“…[49] It provides specific information on macromolecules, i.e., nucleic acids, proteins, and lipids. [50,51] Their structure, composition, and conformation which is unique to each macromolecule which therefore provides total biochemical state like finger printing of molecule. [52][53][54] Sample preparation is mostly simple and reagent free, and material tested with a Raman can be low as or femtoliters or picograms.…”

Section: Rsmentioning

confidence: 99%

Biomarker Detection of Neurological Disorders through Spectroscopy Analysis

Khan¹,

Ahmed²,

Khalid³

et al. 2018

IDJAR

View full text Add to dashboard Cite

Background:The accurate and efficient diagnosis at the early stages of neurological disorders is the key feature for effective treatment and productive research for finding out new ways to combat diseases. It is essentially true for neurological disorders where there is no effective cure, but only treatments are available for slowing down the procedure. Neurological disorders reveal only non-specific clinical symptoms of mental changes/decline starting from a few days to decades after initiation which goes very challenging to differentiate even at later stages when the disorder becomes way aggressive. Despite the fact of having great need, the current availability of diagnostic tests is unable to diagnose different forms of neurological disorders. Aim: The aim of this review is to explore the application of Raman spectroscopy (RS) and mass spectrometry (MS) for the detection of changes in the biochemical composition of cerebrospinal fluid (CSF), blood serum, urine, and saliva. The approach will be based on probing biochemical composition of a biofluid totally using the spectroscopy analysis with advanced statistics. The power of high differentiation method will promote the translation of RS from mere a laboratory technique to clinically useful tool. Demonstration of biochemical information derived from RS from CSF, blood, saliva, and urine that will yield accurate and selective detection of neurological disorders. It will also provide diagnostic and prognostic indicators and will also play a significant role in the development of personalized medicine. Conclusion: Combination of RS and other techniques such as MS and advanced molecular techniques will allow differentiating CSF, blood serum, saliva, and urine samples of common neurological disorders from normal control patients with sensitivity and specificity close to 95%. Clinical Significance: The outcome of the research methods explained will demonstrate an accurate discriminative method which will be based on RS for the detection of neurological disorders. The findings of research in the review will furthermore confirm that if the biomedical application of the methods will either allow to distinguish and detect biomarkers of neurological disorders from biofluids and is it a viable clinical tool that can be used for an accurate diagnosis through a simple test.

show abstract

“…In outline, the representative Raman spectra of blood, semen, vaginal fluid, saliva and sweat have been proposed as shown in Figure 3, information about their chemical composition has been obtained from the study of their spectral bands and several studies proving the differentiation of these five body fluids based on their characteristic Raman signatures by using chemometrics have been published [33][34][35][36][37][38]. Moreover, multivariate classification models have been developed in order to identify and classify unknown fluids, even in mixtures [39][40][41]. Nevertheless, the stains that were analysed in most of these studies were samples of body fluids on glass slides, not mimicking correctly real crime scene samples.…”

Section: Vibrational Spectroscopy In Promising Approaches For Body Flmentioning

confidence: 99%

“…For example, a large part of the studies were carried out by analysing samples of body fluids on glass slides or directly placed onto the instrument [25,[33][34][35][36][37][38][39][40][41], which is an extremely unusual substrate in real cases. In addition, studies in which stains were prepared on different clothes such as cotton, polyester, acrylic or wool, exclusively correspond to blood [18][19][20][21][22][23]27,30,31] except for studies [24,26].…”

Section: Vibrational Spectroscopy In Promising Approaches For Body Flmentioning

confidence: 99%

Body Fluids and Spectroscopic Techniques in Forensics: A Perfect Match?

Zapata¹,

Gregório²,

García‐Ruiz³

2016

J Forensic Med

View full text Add to dashboard Cite

Human body fluids are of great interest in forensics, due to the possibility to extract their genetic information. At the moment, there is the need to develop a non-destructive, rapid and user-friendly method for the detection and identification of the body fluids usually found at crime scenes: blood, semen, vaginal fluid, saliva, sweat and urine. In this review, the spectroscopic techniques used or being researched on this topic are discussed, taking into account their advantages, limitations and advances. Although, UV-Vis light sources are used worldwide in forensic laboratories for the location of body fluids, they are not selective enough to be confirmatory. HSI, FTIR and Raman spectroscopy seem to be suitable for the identification and discrimination of body fluids, though comprehensive research about some unsolved aspects must be performed first.

show abstract

Multidimensional Raman Spectroscopic Signatures as a Tool for Forensic Identification of Body Fluid Traces: A Review

Cited by 87 publications

References 77 publications

Raman Spectroscopy of Blood and Blood Components

Raman Spectroscopy of Blood and Blood Components

Biomarker Detection of Neurological Disorders through Spectroscopy Analysis

Body Fluids and Spectroscopic Techniques in Forensics: A Perfect Match?

Contact Info

Product

Resources

About