Multidimensional effects of biologically synthesized silver nanoparticles in Helicobacter pylori, Helicobacter felis, and human lung (L132) and lung carcinoma A549 cells

Abstract

Silver nanoparticles (AgNPs) are prominent group of nanomaterials and are recognized for their diverse applications in various health sectors. This study aimed to synthesize the AgNPs using the leaf extract of Artemisia princeps as a bio-reductant. Furthermore, we evaluated the multidimensional effect of the biologically synthesized AgNPs in Helicobacter pylori, Helicobacter felis, and human lung (L132) and lung carcinoma (A549) cells. UV-visible (UV–vis) spectroscopy confirmed the synthesis of AgNPs. X-ray diffraction (XRD) indicated that the AgNPs are specifically indexed to a crystal structure. The results from Fourier transform infrared spectroscopy (FTIR) indicate that biomolecules are involved in the synthesis and stabilization of AgNPs. Dynamic light scattering (DLS) studies showed the average size distribution of the particle between 10 and 40 nm, and transmission electron microscopy (TEM) confirmed that the AgNPs were significantly well separated and spherical with an average size of 20 nm. AgNPs caused dose-dependent decrease in cell viability and biofilm formation and increase in reactive oxygen species (ROS) generation and DNA fragmentation in H. pylori and H. felis. Furthermore, AgNPs induced mitochondrial-mediated apoptosis in A549 cells; conversely, AgNPs had no significant effects on L132 cells. The results from this study suggest that AgNPs could cause cell-specific apoptosis in mammalian cells. Our findings demonstrate that this environmentally friendly method for the synthesis of AgNPs and that the prepared AgNPs have multidimensional effects such as anti-bacterial and anti-biofilm activity against H. pylori and H. felis and also cytotoxic effects against human cancer cells. This report describes comprehensively the effects of AgNPs on bacteria and mammalian cells. We believe that biologically synthesized AgNPs will open a new avenue towards various biotechnological and biomedical applications in the near future.

Characterization of silver nanoparticles by green synthesis method using Pedalium murex leaf extract and their antibacterial activity

“…The band observed at 617 cm -1 is shifted to the lower side at 614 cm -1 , which corresponds to C-Cl stretching in the alkyl group. The strong intense peaks at 1382 cm -1 correspond to C-N stretch vibrations, as well as to the amide I bands of proteins in the leaf extract (Gurunathan et al 2015). The strong bands at 1074 cm -1 are due to ether linkages and suggest the presence of flavanones adsorbed on the surface of metal nanoparticles (Shankar et al 2004).…”

Combination of graphene oxide–silver nanoparticle nanocomposites and cisplatin enhances apoptosis and autophagy in human cervical cancer cells

“…Mitochondrial membrane potential (MMP) was measured, as described previously, using a cationic fluorescent indicator (JC-1; Thermo Fisher Scientific). 25 …”

“…Similarly, the anticancer properties of silver NPs have been investigated in various cancer cell lines, including human breast cancer cells 24 and human lung cancer cells. 25 The synthesis of graphene composites with various metal NPs has evoked much interest, due to their unique properties and enhanced antibacterial activity. 26 In addition, graphene offers a suitable platform for the development of nanocomposites, allowing the combination of nanomaterials with different properties to give novel materials improved or new functionality.…”

“…Studies have reported that AgNPs can induce mitochondria-mediated apoptosis by loss of MMP in A549 human lung-carcinoma cells and also in combination with salinomycin in human ovarian cancer cells. 13,25 Similarly, apoptosis-inducing agents can trigger uncoupling of electron transport from ATP production and lead to MMP loss and ROS production. 60,61 Cis induces remarkable mitochondrial changes in HeLa cells.…”

Effect of Silver Nanoparticle-Mediated Wound Therapy on Biochemical, Hematological, and Histological Parameters

“…Plants, microorganisms, fungi, bacteria, yeast, actinomycetes, etc., are some of the components in the biological system [19], which are designated for synthesizing metal nanoparticles [20][21][22]. The phytoconstituents available in the plant extract (P.E) are kenned to have the capacity of synthesizing metal nanoparticles and are withal utilized as capping agent [23][24][25][26][27][28]. Thus, the plant metabolites act both as reducing and capping agents for metal nanoparticles [29][30][31][32].…”