Multicomponent cellulase production by Cellulomonas biazotea NCIM‐2550 and its applications for cellulosic biohydrogen production

Abstract

Among four cellulolytic microorganisms examined, Cellulomonas biazotea NCIM-2550 can grow on various cellulosic substrates and produce reducing sugar. The activity of cellulases (endoglucanase, exoglucanase, and cellobiase), xylanase, amylase, and lignin class of enzymes produced by C. biazotea was mainly present extracellularly and the enzyme production was dependent on cellulosic substrates (carboxymethyl cellulose [CMC], sugarcane bagasse [SCB], and xylan) used for growth. Effects of physicochemical conditions on cellulolytic enzyme production were systematically investigated. Using MnCl(2) as a metal additive significantly induces the cellulase enzyme system, resulting in more reducing sugar production. The efficiency of fermentative conversion of the hydrolyzed SCB and xylan into clean H(2) energy was examined with seven H(2)-producing pure bacterial isolates. Only Clostridiumbutyricum CGS5 exhibited efficient H(2) production performance with the hydrolysate of SCB and xylan. The cumulative H(2) production and H(2) yield from using bagasse hydrolysate (initial reducing sugar concentration = 1.545 g/L) were approximately 72.61 mL/L and 2.13 mmol H(2)/g reducing sugar (or 1.91 mmol H(2)/g cellulose), respectively. Using xylan hydrolysate (initial reducing sugar concentration = 0.345 g/L) as substrate could also attain a cumulative H(2) production and H(2) yield of 87.02 mL/L and 5.03 mmol H(2)/g reducing sugar (or 4.01 mmol H(2)/g cellulose), respectively.

Decolorization and biodegradation of reactive dyes and dye wastewater by a developed bacterial consortium

“…Utilizing agricultural ADR Average decolorization rate (mg h -1 ), CD complete decolorization, ND no decolorization a Effect of initial pH (at 37°C, static condition, dye concentration 50 mg l -1 ) b Effect of temperature (at pH 8.0, static condition, dye concentration 50 mg l -1 ) c Effect of initial dye concentration (at 37°C, pH 8.0, static condition) SM synthetic medium, ND no decolorization a 10 ml extract of each agricultural residue (1%). The extract was prepared by mixing 1 g of rice husk, rice straw, bagasse powder and wood shavings with 100 ml distilled water individually and the mixtures were autoclaved at 121°C for 20 min lignocellulosic waste (about 1.3 9 10 10 metric tons per year) as a supplement to assist or stimulate the degradation of industrial effluents could act as a promising technology and may resolve the problem of the disposal of agro-residues (Saratale et al 2010).…”

Production and characterization of endoglucanase secreted by Streptomyces capoamus isolated from Caatinga

“…The enzymatic hydrolysis of the cellulosic feedstock has several advantages over the chemical processes because of its potential saccharification efficiency and lower energy consumption (Saratale et al, 2008(Saratale et al, , 2010. During the fermentation process, the biomass-degrading microorganisms secrete extracellular enzymes (Santos, 2013).…”

Produção E Caracterização Enzimática Das Celulases Produzidas Por Actinobactérias.

“…Na ultima década muitos trabalhos tem descrito que o aumento da concentração da fonte de carbono pode proporcionar o aumento na produção do composto de interesse, principalmente no que se trata de enzimas hidrolíticas (GOMEZ et al, 2004). No entanto, ainda não se provou se é a concentração aumentada do substrato que afeta a produção da enzima, se é a falta de outro composto no meio que desperta essa produção ou se são os produtos de degradação ou metabólitos formados durante a fermentação, que estimulam a síntese da enzima (SARATALE et al, 2010).…”