Multicompartmentalized polymersomes for selective encapsulation of biomacromolecules

Fu, Zhikang; Ochsner, Mirjam; Hoog, Hans‐Peter M. de; Tomczak, Nikodem; Nallani, Madhavan

doi:10.1039/c0cc03971c

Cited by 84 publications

(81 citation statements)

References 29 publications

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“…20–23 None of these well-established polymersome formulations, however, generates fully biodegradable vesicles via aqueous self-assembly. A few biodegradable polymersome compositions have been prepared from amphiphilic diblock copolymers of PEO and aliphatic polyesters/polycarbonates using an organic co-solvent/water injection/extraction method.…”

Section: Introductionmentioning

confidence: 99%

Aqueous self-assembly of poly(ethylene oxide)-block-poly(ε-caprolactone) (PEO-b-PCL) copolymers: disparate diblock copolymer compositions give rise to nano- and meso-scale bilayered vesicles

Wei

Ghoroghchian

et al. 2013

Nanoscale

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Nanoparticles formed from diblock copolymers of FDA approved PEO and PCL have generated considerable interest as in vivo drug delivery vehicles. Herein, we report the synthesis of the most extensive family PEO-b-PCL copolymers that vary over the largest range of number-average molecular weights (Mn: 3.6 – 57K), PEO weight fractions (fPEO: 0.08 – 0.33), and PEO chain lengths (0.75–5.8K) reported to date. These polymers were synthesized in order to establish the full range of aqueous phase behaviours of these diblock copolymers and to specifically identify formulations that were able to generate bilayered vesicles (polymersomes). Cryogenic transmission electron microscopy (cryo-TEM) was utilized in order to visualize the morphology of these structures upon aqueous self-assembly of dry polymer films. Nanoscale polymersomes were formed from PEO-b-PCL copolymers over a wide range of PEO weight fractions (fPEO: 0.14 – 0.27) and PEO molecular weights (0.75 – 3.8K) after extrusion of aqueous suspensions. Comparative morphology diagrams, which describe the nature of self-assembled structures as a function of diblock copolymer molecular weight and PEO weight fraction, show that in contrast to micron-scale polymersomes, which form only from a limited range of PEO-b-PCL diblock copolymer compositions, a multiplicity of PEO-b-PCL diblock copolymer compositions are able to give rise to nanoscale vesicles. These data underscore that PEO-b-PCL compositions that spontaneously form micron-sized polymersomes, as well as those that have previously been reported to form polymersomes via a cosolvent fabrication system, provide only limited insights into the distribution of PEO-b-PCL diblocks that give rise to nanoscale vesicles. The broad range of polymersome-forming PEO-b-PCL compositions described herein suggest the ability to construct extensive families of nanoscale vesicles of varied bilayer thickness, providing the ability to tune the timescales of vesicle degradation and encapsulant release based on the intended in vivo application.

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Section: Introductionmentioning

confidence: 99%

Aqueous self-assembly of poly(ethylene oxide)-block-poly(ε-caprolactone) (PEO-b-PCL) copolymers: disparate diblock copolymer compositions give rise to nano- and meso-scale bilayered vesicles

Wei

Ghoroghchian

et al. 2013

Nanoscale

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“…One of the most recent approaches consisted in forming the larger polymersomes by solvent-displacement method (or nanoprecipitation) with a suspension of smaller polymersomes previously formed by film rehydration as a water phase. [14] The drawback of this technique lies essentially in the poor encapsulation yield during nanoprecipitation. To overcome this limitation, other options, such as emulsions or double-emulsion techniques, have been investigated.…”

mentioning

confidence: 99%

Polymersomes in Polymersomes: Multiple Loading and Permeability Control

2011

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“…So far, polymersomes have encapsulated a variety of proteins, including aquaporin Z (Kumar et al, 2007), cytochrome C (CC) (Hvasanov et al, 2011), hemoglobin (Rameez et al, 2008; Li et al, 2012), insulin (Xiong et al, 2007; Christian et al, 2009; Kim H. et al, 2012), immunoglobulin G (Fu et al, 2011), ovalbumin (Stano et al, 2013), and myoglobin (Kishimura et al, 2007). Recent investigations also have found that polymer vesicles with asymmetrical membranes exhibit much higher loading capacity of proteins than symmetrical polymersomes.…”

Section: Applications Of Asymmetric Polymersomesmentioning

confidence: 99%

Asymmetrical Polymer Vesicles for Drug delivery and Other Applications

Zhao

et al. 2017

Front. Pharmacol.

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Scientists have been attracted by polymersomes as versatile drug delivery systems since the last two decades. Polymersomes have the potential to be versatile drug delivery systems because of their tunable membrane formulations, stabilities in vivo, various physicochemical properties, controlled release mechanisms, targeting abilities, and capacities to encapsulate a wide range of drugs and other molecules. Asymmetrical polymersomes are nano- to micro-sized polymeric capsules with asymmetrical membranes, which means, they have different outer and inner coronas so that they can exhibit better endocytosis rate and endosomal escape ability than other polymeric systems with symmetrical membranes. Hence, asymmetrical polymersomes are highly promising as self-assembled nano-delivery systems in the future for in vivo therapeutics delivery and diagnostic imaging applications. In this review, we prepared a summary about recent research progresses of asymmetrical polymersomes in the following aspects: synthesis, preparation, applications in drug delivery and others.

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Multicompartmentalized polymersomes for selective encapsulation of biomacromolecules

Cited by 84 publications

References 29 publications

Aqueous self-assembly of poly(ethylene oxide)-block-poly(ε-caprolactone) (PEO-b-PCL) copolymers: disparate diblock copolymer compositions give rise to nano- and meso-scale bilayered vesicles

Aqueous self-assembly of poly(ethylene oxide)-block-poly(ε-caprolactone) (PEO-b-PCL) copolymers: disparate diblock copolymer compositions give rise to nano- and meso-scale bilayered vesicles

Polymersomes in Polymersomes: Multiple Loading and Permeability Control

Asymmetrical Polymer Vesicles for Drug delivery and Other Applications

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