Multicolour FISH painting for the analysis of chromosomal aberrations induced by 220kV X-rays and fission neutrons

Abstract

Compared with single-colour FISH painting, a multicolour approach provides extended information for a mechanistic and quantitative interpretation of radiation-induced chromosome aberrations. The choice of a nomenclature system and the selection of an appropriate aberration parameter or category depend on these specific aspects. Practical application requires a rapid and reproducible description of the observed painting patterns and should also throw light on the origin of aberrations.

Complex chromosome aberrations persist in individuals many years after occupational exposure to densely ionizing radiation: An mFISH study

“…Thus, these measurements suggest that complex chromosome aberrations may be a useful biomarker of densely ionizing radiation exposure in a human population exposed many years earlier. These results in a human population confirm earlier in vitro–based studies (Griffin et al, 1995; Testard et al, 1997; Knehr et al, 1999; Anderson et al, 2000, 2002, 2003; Boei et al, 2001; Moquet et al, 2001), as well as earlier suggestions (Anderson et al, 2000; Boei et al, 2001) that complex chromosomal aberrations have the potential to be a specific biomarker for past exposure to densely ionizing radiation.…”

“…Chromosome aberrations involving three or more breaks in two or more chromosomes are defined as complex (Savage and Simpson, 1994). In vitro, they have been shown to be preferentially induced after exposure to low doses of densely ionizing radiation such as that of alpha particles or neutrons, compared with sparsely ionizing radiation such as that of X‐rays (Griffin et al, 1995; Testard et al, 1997; Knehr et al, 1999; Anderson et al, 2000, 2002, 2003; Boei et al, 2001; Moquet et al, 2001). This preferential production of complex aberrations by densely ionizing radiation is related to the unique energy deposition patterns produced by densely ionizing radiation, which produces highly localized multiple DNA damage at the chromosomal level (Brenner and Ward, 1992; Prise et al, 2001; Anderson et al, 2002).…”

Chromosome Damage Caused by Accidental Chronic Whole-Body Gamma Radiation Exposure in Thailand

“…[35][36][37][38][39][40][41] However, some studies have observed a higher frequency of symmetrical translocations. [42][43][44][45][46][47] We observed a similar frequency at the first mitosis of symmetrical translocations compared to asymmetrical translocations following in vitro exposures (data not shown).…”

Radiation Dose of the Lens in Trans-sphenoidal Pituitary Surgery: Pros and Cons of a Conventional Setup Using Fluoroscopic Guidance and CT-Based Neuronavigation

“…Irradiation of blood with doses as low as 0.2 Gy by using x-rays demonstrated doseresponse curves of chromosomal aberrations. 26 In all published experimental studies with rodents, it was difficult or impossible to determine excess tumor rates at doses substantially less than 1 Gy. 24 Factors such as variations in radiosensitivity during the cell cycle; induction of an adaptive response to an initial exposure, which can reduce the effect of later exposures; bystander effect; induction of persistent genomic instability; and hyper-radiation sensitivity may already be caused by low LET radiation.…”