Multicolor structured illumination microscopy and quantitative control of polychromatic coherent light with a digital micromirror device

Brown, Peter; Kruithoff, Rory; Seedorf, Gregory J.; Shepherd, Douglas P.

doi:10.1101/2020.07.27.223941

Cited by 6 publications

(9 citation statements)

References 67 publications

(99 reference statements)

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…The results provide practical design suggestions for circumventing the undesired blazed-grating effect of DMDs. Our simulation results agree with the independent findings of Brown et al, who were able to successfully construct a multi-colour DMD SIM offering three excitation wavelengths [22]. Following our analysis of suitable wavelength, and building on top of an existing opto-mechanical platform [14], it should now also be possible to design a multicolour DMD-based SIM microscope with simple, passive opto-mechanics which can fully use all of the speed and cost advantages of a DMD mentioned before.…”

Section: Discussionsupporting

confidence: 91%

“…0.3°in [14]). Brown et al were able to construct such a system [22] offering three excitation wavelengths (they chose a triplet of 473 nm, 532 nm and 635 nm). They use a (semi-)analytic approach to model the blazed grating effect, developed independently of the approaches presented here, while providing compatible results, which we view as an important cross-check.…”

Section: Resultsmentioning

confidence: 99%

“…Harvey & Pfisterer [25] provide a one-dimensional introduction to the blazed grating effect itself, but this model needs to be extended when used to describe a two-dimensional DMD with mirrors tilting along their diagonal. A white paper [23] by Texas Instruments (itself a large producer of DMD chips) offers an introduction to the blazed grating effect in conjunction with DMDs, and Brown et al [22] recently provided a (semi-)analytic framework to model DMDs. Simultaneously, we started from a purely numerical approach to simulate light propagation in a DMD-based system, and developed simplifications to this model in the process.…”

Section: Methodsmentioning

confidence: 99%

“…They also offer high switching speeds, they can handle high light intensities, and depending on coating, are not sensitive to light polarization. This makes them an interesting option for many SLM applications in microscopy [14][15][16][17][18][19][20][21][22]. However, the jagged nature of the micromirror array gives rise to the blazed grating effect that becomes rather annoying and detrimental when using DMDs in combination with a coherent light source [23,24].…”

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

Simulating digital micromirror devices for patterning coherent excitation light in structured illumination microscopy

Lachetta

Sandmeyer

et al. 2021

Phil. Trans. R. Soc. A.

View full text Add to dashboard Cite

Digital micromirror devices (DMDs) are spatial light modulators that employ the electro-mechanical movement of miniaturized mirrors to steer and thus modulate the light reflected off a mirror array. Their wide availability, low cost and high speed make them a popular choice both in consumer electronics such as video projectors, and scientific applications such as microscopy. High-end fluorescence microscopy systems typically employ laser light sources, which by their nature provide coherent excitation light. In super-resolution microscopy applications that use light modulation, most notably structured illumination microscopy (SIM), the coherent nature of the excitation light becomes a requirement to achieve optimal interference pattern contrast. The universal combination of DMDs and coherent light sources, especially when working with multiple different wavelengths, is unfortunately not straight forward. The substructure of the tilted micromirror array gives rise to a blazed grating, which has to be understood and which must be taken into account when designing a DMD-based illumination system. Here, we present a set of simulation frameworks that explore the use of DMDs in conjunction with coherent light sources, motivated by their application in SIM, but which are generalizable to other light patterning applications. This framework provides all the tools to explore and compute DMD-based diffraction effects and to simulate possible system alignment configurations computationally, which simplifies the system design process and provides guidance for setting up DMD-based microscopes. This article is part of the Theo Murphy meeting ‘Super-resolution structured illumination microscopy (part 1)’.

show abstract

Section: Discussionsupporting

confidence: 91%

Section: Resultsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Simulating digital micromirror devices for patterning coherent excitation light in structured illumination microscopy

Lachetta

Sandmeyer

et al. 2021

Phil. Trans. R. Soc. A.

View full text Add to dashboard Cite

show abstract

“…They also offer high switching speeds, they can handle high light intensities, and depending on coating, are not sensitive to light polarization. This makes them an interesting option for many SLM applications in microscopy [14–22]. However, the jagged nature of the micromirror array gives rise to the blazed grating effect that becomes rather annoying and detrimental when using DMDs in combination with a coherent light source [23,24].…”

Section: Introductionmentioning

confidence: 99%

Simulating digital micromirror devices for patterning coherent excitation light in structured illumination microscopy

Lachetta

Sandmeyer

et al. 2020

Preprint

View full text Add to dashboard Cite

SummaryDigital micromirror devices (DMDs) are spatial light modulators that employ the electro-mechanical movement of miniaturized mirrors to steer and thus modulate the light reflected of a mirror array. Their wide availability, low cost and high speed make them a popular choice both in consumer electronics such as video projectors, and scientific applications such as microscopy.High-end fluorescence microscopy systems typically employ laser light sources, which by their nature provide coherent excitation light. In super-resolution microscopy applications that use light modulation, most notably structured illumination microscopy (SIM), the coherent nature of the excitation light becomes a requirement to achieve optimal interference pattern contrast. The universal combination of DMDs and coherent light sources, especially when working with multiple different wavelengths, is unfortunately not straight forward. The substructure of the tilted micromirror array gives rise to a blazed grating, which has to be understood and which must be taken into account when designing a DMD-based illumination system.Here, we present a set of simulation frameworks that explore the use of DMDs in conjunction with coherent light sources, motivated by their application in SIM, but which are generalizable to other light patterning applications. This framework provides all the tools to explore and compute DMD-based diffraction effects and to simulate possible system alignment configurations computationally, which simplifies the system design process and provides guidance for setting up DMD-based microscopes.

show abstract

Answering some questions about structured illumination microscopy

Manton

2022

Phil. Trans. R. Soc. A.

View full text Add to dashboard Cite

Structured illumination microscopy (SIM) provides images of fluorescent objects at an enhanced resolution greater than that of conventional epifluorescence wide-field microscopy. Initially demonstrated in 1999 to enhance the lateral resolution twofold, it has since been extended to enhance axial resolution twofold (2008), applied to live-cell imaging (2009) and combined with myriad other techniques, including interferometric detection (2008), confocal microscopy (2010) and light sheet illumination (2012). Despite these impressive developments, SIM remains, perhaps, the most poorly understood ‘super-resolution’ method. In this article, we provide answers to the 13 questions regarding SIM proposed by Prakash et al. along with answers to a further three questions. After providing a general overview of the technique and its developments, we explain why SIM as normally used is still diffraction-limited. We then highlight the necessity for a non-polynomial, and not just nonlinear, response to the illuminating light in order to make SIM a true, diffraction-unlimited, super-resolution technique. In addition, we present a derivation of a real-space SIM reconstruction approach that can be used to process conventional SIM and image scanning microscopy (ISM) data and extended to process data with quasi-arbitrary illumination patterns. Finally, we provide a simple bibliometric analysis of SIM development over the past two decades and provide a short outlook on potential future work. This article is part of the Theo Murphy meeting issue ‘Super-resolution structured illumination microscopy (part 2)’.

show abstract

Multicolor structured illumination microscopy and quantitative control of polychromatic coherent light with a digital micromirror device

Cited by 6 publications

References 67 publications

Simulating digital micromirror devices for patterning coherent excitation light in structured illumination microscopy

Simulating digital micromirror devices for patterning coherent excitation light in structured illumination microscopy

Simulating digital micromirror devices for patterning coherent excitation light in structured illumination microscopy

Answering some questions about structured illumination microscopy

Contact Info

Product

Resources

About