Multicolor Imaging of Cancer Cells with Fluorophore-Tagged Aptamers for Single Cell Typing

Wang, Song; Kong, Hao; Gong, Xiaoyun; Zhang, Sichun; Zhang, Xinrong

doi:10.1021/ac501657g

Cited by 36 publications

(24 citation statements)

References 33 publications

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“…Biochemical analysis at the single-cell level can provide information for more precise diagnose and therapy. [10][11][12][13][14] We thus explored vitamin B 2 nanorods with ahigh aspect ratio for single-cell phototherapy.F igure 4A shows optical waveguiding using as ingle vitamin B 2 nanorod at the single-cell level. In detail, when light is introduced from one point far away from the tumor cell, the fluorescence emission can be delivered into the inside of cell, although the intensity decreases to some degree.M oreover,F igure 4B reveals that after spot light illumination for 5min, the tumor cell near asingle nanorod is killed, as evidenced by the bright emission from PI, which specially stains the nuclei of dead cells.…”

Section: Angewandte Chemiementioning

confidence: 99%

Assembled Vitamin B2 Nanocrystals with Optical Waveguiding and Photosensitizing Properties for Potential Biomedical Application

et al. 2019

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Great success has been achieved in recent years in the development of synthetic or assembled nanobiomaterials. Among these,b iomolecule-based nanoarchitectures with special optical property are of particular interest. Here,w e demonstrate that vitamin B 2 nanocrystals assembled as nanorods can be obtained with precise control. Excitingly,such onedimensional nanostructures not only exhibit intrinsic optical waveguiding properties but also the ability to sensitizeo xygen to produce reactive oxygen species.W ith these properties,w e applied the obtained vitamin B 2 nanorods under remotely localized light illumination into single tumour cells in vitro for anticancer photodynamic therapy. Further,v itamin B 2 nanorods were explored for in vivo photodynamic therapybyusing at umour model. With such bionanostructures,n ew features and functions of vitamin B 2 and its derivatives have been developed.

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Section: Angewandte Chemiementioning

confidence: 99%

Assembled Vitamin B2 Nanocrystals with Optical Waveguiding and Photosensitizing Properties for Potential Biomedical Application

et al. 2019

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“…Various receptor systems have been employed for array-based sensing of cells, including fluorescent polymers [53], green fluorescent proteins [46, 50, 55], fluorescently labeled DNAs [52, 54], magnetic glyco-nanoparticles [51], and gold nanomaterials [48, 49]. Although these methods are capable of discerning cells, these systems generally require a large population of cells.…”

Section: Introductionmentioning

confidence: 99%

Pattern-based sensing of triple negative breast cancer cells with dual-ligand cofunctionalized gold nanoclusters

Tao

Auguste

2017

Biomaterials

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Early detection of breast cancer is a critical component in patient prognosis and establishing effective therapy regimens. Here, we developed an easily accessible yet potentially powerful sensor to detect cancer cell targets by utilizing seven dual-ligand cofunctionalized gold nanoclusters (AuNCs) as both effective cell recognition elements and signal transducers. On the basis of this AuNC multichannel sensor, we have successfully distinguished healthy, cancerous and metastatic human breast cells with excellent reproducibility and high sensitivity. Triple negative breast cancer cells (TNBCs), which exhibit low expression of the estrogen receptor, progesterone receptor, and human epidermal growth factor receptor-2, were identified. The high accuracy of the blind breast cell sample tests further validates the practical application of the sensor array. In addition, the versatility of the sensor array is further justified by identifying amongst distinct cell types, different cell concentrations and cell mixtures. Notably, the drug-resistant cancer cells can also be efficiently discriminated. Furthermore, the dual-ligand cofunctionalized AuNCs can efficiently differentiate different cells from the peripheral blood of tumor-free and tumor-bearing mice. Taken together, this fluorescent AuNCs based array provides a powerful cell analysis tool with potential applications in biomedical diagnostics.

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“…As reported, the cross-reactive signals are crucial important for the fabrication of sensor array. [34][35][36][37][38][39] As resulted, we obtained different CL signals with the presence of different metal ions in C-dots solutions. For example, we recorded the enhanced CL signals with the presence of Zn 2+ , the decreased signals for Fe 3+ , and the almost similar CL signals for Cr 3+ compared with the C-dots solution without any metal ions added ( Figure 6A).…”

Section: Characterization Of the Ltp-treated C-dotsmentioning

confidence: 87%

Excited Oxidized-Carbon Nanodots Induced by Ozone from Low-Temperature Plasma to Initiate Strong Chemiluminescence for Fast Discrimination of Metal Ions

et al. 2016

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Carbon nanodots (C-dots) are recently well examined due to the emissions with color-tuning and nonblinking properties, while more studies are still needed for the appropriate understanding and application of distinct emissions. In this work, we found the emission of chemiluminescence (CL) by introducing low-temperature plasma (LTP) into C-dots solutions without any reagent added, whose intensity was affected by the presence of different metal ions. Based on both experimental data and theoretical calculations, we found with the ozonation by ozone from LTP, excited oxidized-C-dots would be generated with the addition of ozone onto the conjugated double bonds of C-dots, and these excited species could directly initiate strong CL combining with the deactivation of excited species to the ground state. Significantly, the cross-reactive CL signals were obtained from different kinds of C-dots with the presence of different metal ions. Therefore, a new sensor array (electronic tongue) composed of five different C-dots was designed for fast discrimination of metal ions, which achieved the accurate discrimination of 13 kinds of metal ions in pure water and real samples. It exhibited good reproducibility and sensitivity, which can be used for the quantitative analysis of metal ions such as showing a linear range from 4 × 10(-7) to 6 × 10(-5) mol·L(-1) (R(2) > 0.99) for Fe(3+) with a detection limit of 2.5 × 10(-7) mol·L(-1). This work not only provides a novel finding of CL from C-dots revealing explicit relationship between structures and CL properties, but also realizes the fast discrimination of metal ions, showing potentials in environmental monitoring and quality identifications.

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Multicolor Imaging of Cancer Cells with Fluorophore-Tagged Aptamers for Single Cell Typing

Cited by 36 publications

References 33 publications

Assembled Vitamin B2 Nanocrystals with Optical Waveguiding and Photosensitizing Properties for Potential Biomedical Application

Assembled Vitamin B2 Nanocrystals with Optical Waveguiding and Photosensitizing Properties for Potential Biomedical Application

Pattern-based sensing of triple negative breast cancer cells with dual-ligand cofunctionalized gold nanoclusters

Excited Oxidized-Carbon Nanodots Induced by Ozone from Low-Temperature Plasma to Initiate Strong Chemiluminescence for Fast Discrimination of Metal Ions

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