Multicolor Barcoding in a Single Upconversion Crystal

Zhang, Yuhai; Zhang, Lixin; Deng, Renren; Tian, Jing; Zong, Yun; Jin, Dong-Yan; Liu, Xiaogang

doi:10.1021/ja5013646

Cited by 352 publications

(253 citation statements)

References 58 publications

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“…Inherited from the studies on fluorescent dyes and quantum dots, optical multiplexing strategies using UCNPs have been developed in the spectral domain 7,[24][25][26][27] , time domain 28 and spatial domain 29,30 . However, studies of the complex luminescence mechanisms involving sequential multi-step photophysical and energy transfer processes featuring complex luminescence kinetics are still at their infancy [31][32][33][34] , and may open possibility to develope unique multiplexing strategies in other domains.…”

Section: Introductionmentioning

confidence: 99%

Phase angle encoded upconversion luminescent nanocrystals for multiplexing applications

et al. 2017

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Lanthanide-doped upconversion nanoparticles (UCNPs) are increasingly used as luminescent candidates in multiplexed applications due to their excellent optical properties. Inthepast,several encodingidentities havebeen proposedforUCNPs,includingemissioncolour,intensity ratio between different emissionbands, colourspatial distribution, and luminescencelifetime.In this paper, a new optical encoding dimension for upconversion nanomaterials is developed by exploring their luminescence kinetics, i.e., the phase angle of upconversion luminescence in response to a harmonic-wave excitation. Our theoretical derivation shows that the phase angle is governed jointly by the rise and decay times, characterizing the upconversion luminescence kinetics. Experimentally, a full set of methods are developed to manage the upconversion luminescence kinetics, through which the rise and decay times can be manipulated dependently or independently. Furthermore,a large phase-angle space is achieved in which tens of unique codes can be potentially generated in the same colour channel. Our work greatly extends the multiplexing capacity of UCNPs,and offers newopportunities for their applicationsin a wide range such as microarray assays, bioimaging, anti-counterfeiting, deep tissue multiplexed labelling/detectionand high-density data storage.In addition, the development of thisluminescence kinetics-based optical encoding strategy is also instructive for developing multiplexing techniques using other cascade luminescent systems that inherently lack multi-spectral channels, such as triplet-triplet annihilation molecule pairs.

QC 20170330

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Section: Introductionmentioning

confidence: 99%

Phase angle encoded upconversion luminescent nanocrystals for multiplexing applications

et al. 2017

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QC 20170330

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“…However, the majority of NCs that can be made via alternative synthesis methods cannot be grown in-situ in glass. For example, it is not possible to grow nanodiamond or heterogeneous nanostructures in glass, which limits the ability to engineering optical properties at the nanoscale [56][57][58][59][60]. For NCs that can be grown in-situ, it is still challenging to reach a high level of compositional and nanostructural control over the NCs.…”

Section: Nanoparticle-doped Glasses and Fibersmentioning

confidence: 99%

Glass and Process Development for the Next Generation of Optical Fibers: A Review

Ballato

Ebendorff‐Heidepriem

Zhao

et al. 2017

Fibers

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Applications involving optical fibers have grown considerably in recent years with intense levels of research having been focused on the development of not only new generations of optical fiber materials and designs, but also on new processes for their preparation. In this paper, we review the latest developments in advanced materials for optical fibers ranging from silica, to semi-conductors, to particle-containing glasses, to chalcogenides and also in process-related innovations.

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“…-doped UCNPs, many interesting UCL properties such as multicolor emission, single-band emission, broad-band emission, stimulated emission and lasing, and UCL lifetime multiplexing have been explored [144][145][146][147][148][149][150][151][152][153] [164][165][166][167][168][169][170][171][172]. In particular, the newly-developed Nd 3+ -sensitized UCNPs are more advantageous for in vivo bioimaging than common Yb 3+ -sensitized UCNPs, since the laser-induced tissue overheating effect can be minimized by virtue of the Nd 3+ absorption at ~808 nm where the water absorption cross-section is much smaller than that at 980 nm [168][169][170][171][172].…”

Section: +mentioning

confidence: 99%

Inorganic lanthanide nanoprobes for background-free luminescent bioassays

Huang

Zheng

et al. 2015

Sci. China Mater.

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Luminescent bioassay techniques have been widely adopted in a variety of research and medical institutions. However, conventional luminescent bioassays utilizing traditional bioprobes like organic dyes and quantum dots often suffer from the interference of background noise from scattered lights and autofluorescence from biological matrices. To eliminate this disadvantage, the use of inorganic lanthanide (Ln 3+ )-doped nanoparticles (NPs) is an excellent option in view of their superior optical properties, such as the long-lived downshifting luminescence, near-infrared triggered anti-Stokes upconverting luminescence and excitation-free persistent luminescence. In this review, we summarize the latest advances in the development of inorganic Ln 3+ -doped NPs as sensitive luminescent bioprobes from their fundamental physicochemical properties to biodetection, including the chemical synthesis, surface functionalization, optical properties and their promising applications for background-free luminescent bioassays. Future efforts and prospects towards this rapidly growing field are also proposed. INTRODUCTIONSensitive and specific bioassay of trace amount of target analytes is essential for a variety of biomedical applications ranging from pharmaceutical preparation to disease diagnosis and therapy [1][2][3]. Among various bioassay methods, luminescent bioassay has received particular attention because of its high sensitivity and good specificity [4,5]. Conventional luminescent bioassay techniques such as enzyme-linked immunosorbent assay (ELISA), time-resolved (TR) fluoroimmunoassay (TRFIA), Förster resonance energy transfer (FRET) and TR-FRET assays have laid the foundation for many modern clinical applications [6][7][8][9][10][11]. However, these bioassays are impeded by the availability of traditional bioprobes like organic dyes, lanthanide (Ln 3+ ) chelates and quantum dots (QDs). The use of these bioprobes has a number of limitations. Organic dyes commonly possess poor photochemical stability and suffer from serious photobleaching [12]. The applicability of QDs is compromised by photoblinking and high toxic- ity of heavy metal elements like cadmium and selenium, especially for in vivo biosensing [13,14]. Moreover, both organic dyes and QDs may induce high background noise and considerable photodamage to the biological samples under ultraviolet (UV) excitation, which deteriorates their detection sensitivity for bioassays [15,16]. Although such background noise can be suppressed by the technique of TR photoluminescence (PL) through the use of the longlived luminescence of Ln 3+ -chelates, the poor photochemical stability, long-term toxicity and high cost of Ln 3+ -chelates remain a major issue [17]. These concerns fuel a crucial demand for the development of a new generation of luminescent bioprobes to circumvent the limitations of traditional ones.Recently, with the explosion of nanoscience and nanotechnology, there is a growing dedication towards the development of diverse luminescent nanoprobes for biodetection ...

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Multicolor Barcoding in a Single Upconversion Crystal

Cited by 352 publications

References 58 publications

Phase angle encoded upconversion luminescent nanocrystals for multiplexing applications

Phase angle encoded upconversion luminescent nanocrystals for multiplexing applications

Glass and Process Development for the Next Generation of Optical Fibers: A Review

Inorganic lanthanide nanoprobes for background-free luminescent bioassays

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