Multiclass pesticide residue analysis in fish muscle and liver on one-step extraction-cleanup strategy coupled with liquid chromatography tandem mass spectrometry

Kaczyński, Piotr; Łozowicka, Bożena; Perkowski, Maciej; Szabunko, Julia

doi:10.1016/j.ecoenv.2016.12.040

Cited by 64 publications

(21 citation statements)

References 43 publications

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“…In the case of matrix-matched calibration, recoveries were in accordance with results reported by Kaczyński et al for 300 pesticides in fish tissues (Kaczyński, Łozowicka, Perkowski, & Szabuńko, 2017) or by Mol et al for 258 molecules in different types of food (Hans G. J. Mol et al, 2008), with around 63-78% of molecules within the recovery range 70-120% (see detailed results in Table 2).…”

Section:  Recoveriessupporting

confidence: 89%

Multi-class analysis for simultaneous determination of pesticides, mycotoxins, process-induced toxicants and packaging contaminants in tea

et al. 2018

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This study attempts at uniting the analysis of four different classes of contaminants for both liquid and solid tea samples. A total of 32 compounds, classified as pesticides, mycotoxins, process-induced toxicants or packaging contaminants, were carefully chosen for their diversity of structures and physicochemical properties. The proposed method combines a sample treatment strategy coming from metabolomics with liquid chromatography analysis using a silica bonded C18-pentafluorophenyl column coupled to high resolution mass spectrometry. For tea brew, dilute and shoot method provides good quantification (70-120% recoveries and <20% RSD) for more than 80% of compounds. For tea leaves, strong matrix effects are observed, thus, matrix-matched calibration is required to reach good performances, i.e. 63% of compounds quantified and 81% detected at 10µg/kg. Finally, method performances were evaluated against existing regulations, and it appears that 69% of contaminants are quantified and 91% detected at levels lower than their respective European regulation limits.

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Section:  Recoveriessupporting

confidence: 89%

Multi-class analysis for simultaneous determination of pesticides, mycotoxins, process-induced toxicants and packaging contaminants in tea

et al. 2018

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“…On the other hand, solvent extractions proved to be suitable for the analysis of phosalone, propargite 67 and tolyfluanid in liver samples [24][25][26]. 68…”

Section: Samples 66mentioning

confidence: 99%

“…Once these potentially contaminated 44 fruits have been consumed and the compounds have passed into the body, these latter are transported by 45 the blood flow to the liver for metabolization [24]. Therefore, in the scope of metabolism [24] or 46 biomonitoring [25][26][27] studies, a sensitive and reliable analytical method is needed to assess the 47 pesticides level in liver fractions. Until now, as far as we know, bibliographic research shows that no 48 work has been published on the simultaneous determination of diphenylamine, phosalone, propargite 49 and tolylfluanid in human biological samples.…”

Section: Introduction 31mentioning

confidence: 99%

“…Until now, as far as we know, bibliographic research shows that no 48 work has been published on the simultaneous determination of diphenylamine, phosalone, propargite 49 and tolylfluanid in human biological samples. including the determination of propargite and phosalone [24] or the quantification of propargite and 52 tolylfluanid [25] in fish liver. These methods were based on the use of liquid chromatography coupled 53 to tandem mass spectrometry (LC-MS/MS).…”

Section: Introduction 31mentioning

confidence: 99%

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A simple GC–MS method for the determination of diphenylamine, tolylfluanid propargite and phosalone in liver fractions

Kadar

Peyre²,

Wortham

et al. 2019

Journal of Chromatography B

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Pierre Doumenq. A simple GC-MS method for the determination of diphenylamine, tolylfluanid propargite and phosalone in liver fractions. Journal of Chromatography B Biomedical Sciences and Applications, Elsevier, 2019, 1113, pp.(A. Kadar) and ludovic.peyre-6 teisseire@hotmail.fr (L. Peyre) 7 8 ABSTRACT 9In this study, an accurate and robust gas chromatography/mass spectrometry method was developed for 10 quantitative analysis of diphenylamine, tolylfluanid, propargite and phosalone in liver fractions. 11Different injector parameters were optimized by an experimental design of experiment technique 12 (central composite design). An optimal combination of injector temperature (°C), splitless time (min) 13 and overpressure (KPa) values enabled to maximize the chromatographic responses. Sample preparation 14 was based on protein precipitation using trichloroacetic acid followed by liquid-liquid extraction (LLE) 15 of the pesticides with hexane. All compounds were quantified without interference in selected ion 16 monitoring mode using endrin as internal standard. The calibration curves for diphenylamine, 17 tolylfluanid, propargite and phosalone compounds were linear over the concentration range of 18 0.1 to 25 µM (i.e.0.1, 0.5, 2.0, 5.0, 15.0 and 25.0 µM) ) with determination coefficients (R 2 ) higher than 19 0.999. A lower limit of quantification of 0.1 µM was obtained for all analytes, i.e. 422.5, 868.0, 876.2 20 and 919.5 µg/kg of liver fraction (hepatocytes) for diphenylamine, tolylfluanid, propargite and 21 phosalone, respectively. All compounds showed extraction recoveries higher than 93%, with a 22 maximum RSD of 3.4%. Intra-and inter-day accuracies varied from 88.4 to 102.9% and, imprecision 23 varied from 1.1 to 6.7%. Stability tests demonstrated that all pesticides were stable in liver extracts 24 during instrumental analysis (20°C in the autosampler tray for 72 h) following three successive freeze-25 thaw cycles and, at -20°C for up to 12 months. This simple and efficient analytical procedure is thus 26 suitable for assessing mammals liver contamination or metabolism studies. 27

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“…Analytical methods for the analysis of polychlorinated biphenyls (PCBs) and organochlorine pesticides (OCPs) are widely available and are the result of a vast amount of environmental analytical method development and research on persistent organic pollutants (POPs) over the past 30-40 years (Muir et al, 2006). Even so, the analysis of pesticide residues in fish samples is challenging due to the low concentrations and large number of analytes that need to be monitored and quantified in a complex matrix (Kaczyński et al, 2017, Rodrigues et al, 2016. Usually a solvent-based extraction of the sample is required in the first part of the pesticide determination process from which will result contaminants and unwanted matrices.…”

Section: Introductionmentioning

confidence: 99%

The Development of an Automated Clean-up for Fat Extracts in the Routine Analysis of Organochlorine Compounds in Fish Meat

Cioca¹,

Heemken²,

Mihaiu³

2017

BUASVMCN-VM

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The present work describes the development of a new, automatic High Performance Liquid Chromatography (HPLC) Clean-up step, in the methodology of sample preparation and multi-residue determination of organochlorine compounds (OCs) in fish meat. 24 OCswere taken into study. In addition 7 Polychlorinated Biphenyls (PCBs), 7 chlorobenzene compounds and one 2,3,7,8-Tetrachlorodibenzo-p-dioxin (TCDD) were investigated.The HPLC conditions were established in accordance with the validated traditional Clean-up step of the laboratory. The technique was applied on a dilution of analytes of interest in order to establish the period of time in which the compounds are eluted. Another set of experiments involved fish oil, in order to identify and separate the fat fraction from the analytes. To confirm the findings of the experiments mentioned above, extracts of fish samples obtained after Accelerated Solvent Extraction (ASE) were examined. The samples were spiked with the analytes of interest before HPLC clean-up step and quantified throughGas Chromatography coupled with tandem Mass Spectrometry (GC-MS/MS). A HPLC clean-up technique lasting 38 minutes/ sample was developed. The method is not suitablefor OCs such as Endosulfansulfat and Endrine Ketonedue to the very low recovery results.

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Multiclass pesticide residue analysis in fish muscle and liver on one-step extraction-cleanup strategy coupled with liquid chromatography tandem mass spectrometry

Cited by 64 publications

References 43 publications

Multi-class analysis for simultaneous determination of pesticides, mycotoxins, process-induced toxicants and packaging contaminants in tea

Multi-class analysis for simultaneous determination of pesticides, mycotoxins, process-induced toxicants and packaging contaminants in tea

A simple GC–MS method for the determination of diphenylamine, tolylfluanid propargite and phosalone in liver fractions

The Development of an Automated Clean-up for Fat Extracts in the Routine Analysis of Organochlorine Compounds in Fish Meat

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