Multiclass and multiresidue screening of veterinary drugs and pesticides in infant formula using Quadrupole‐Orbitrap MS with PRM scan mode

Abstract

A multiclass and multiresidue method for screening veterinary drugs and pesticides in infant formula was developed and validated using ultrahigh‐performance liquid chromatography coupled to Quadrupole‐Orbitrap high‐resolution mass spectrometry (UHPLC‐HRMS). A total of 49 veterinary drugs and pesticides investigated belong to 11 classes including antivirals, anticoccidials, macrolides, pyrethroids, insecticides, sulfonamides, beta‐agonists, sedatives, thyreostats, nonsteroidal anti‐inflammatory drugs, and other pharmacologically active substances. A generic sample preparation and highly selective acquisition mode of parallel reaction monitoring (PRM) were deliberately incorporated to perform efficient screening analysis. As a result, the screening target concentrations of the analytes varied from 1 to 500 μg/kg with ≤5% of false compliant rate as specified in Decision 2002/657/EC for screening analysis. The average recoveries ranged from 40.7 to 124.9% as well as the relative standard deviations from 4.2 to 26.6%, respectively. The matrix effects and interferences were effectively controlled by integrated application of dispersive solid phase extraction, PRM scan mode, and matrix‐matched standard calibration. The proposed method will be helpful to provide applicable strategy for screening residues in infant formula with surveillance purpose.

Advances in high‐resolution mass spectrometry applied to pharmaceuticals in 2020: A whole new age of information

“…This method incorporated 49 compounds belonging to 11 different compound classes. 67 For the profiling and authentication of Ginseng in traditional Chinese medicine, Zhang et al used ion mobility spectrometry (IMS) for the development of a UHPLC-IMS-Q-TOF-MS method for the added orthogonal separation of ginsenosides to more confidently distinguish the peaks of interest. For method optimization, the group investigated the effects of changing the tolerance (low/high) of both the CCS filtering for the ion mobility cell and m/z filtering for the quadrupole to effectively identify and avoid interfering substances (such as isomers) in the final method conditions.…”

“…developed and validated a targeted screening method using UHPLC‐Q‐Orbitrap‐MS to detect veterinary drugs and pesticides in infant formula for safety and quality control purposes. This method incorporated 49 compounds belonging to 11 different compound classes 67 …”