Multichannel electroanalytical devices for competitive ELISA of phenylethanolamine A

Liu, Yang; Cai, Meng; Wu, Wenbing; Fang, Yuting; She, Pei; Xu, Shouming; Li, Jianguo; Zhao, Kang; Xu, Jing‐Juan; Bao, Ning; Deng, Anping

doi:10.1016/j.bios.2017.04.002

Cited by 14 publications

(4 citation statements)

References 51 publications

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“…The most commonly used detection system for ELISA is the colorimetric one. In order to improve the analytical figures of merit of immunoassay detection systems, various detection principles have been applied such as chemiluminescence [26], electrochemistry [27], surface plasmon resonance [28], Raman scattering spectroscopy [29], and fluorescence spectroscopy [30]. Among them, fluorescence is a good option due to its high detection sensitivity and fast response times [31,32].…”

Section: Accepted Manuscriptmentioning

confidence: 99%

Ethinylestradiol quantification in drinking water sources using a fluorescent paper based immunosensor

Scala-Benuzzi

Takara

Alderete

et al. 2018

Microchemical Journal

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In this work we report a novel paper-based analytical device read-out via LEDinduced fluorescence detection (FPAD) for the quantification of the emerging pollutant ethinylestradiol (EE2) in river water samples. The PAD was used as a reaction platform for a competitive enzyme immunoassay. For the PAD development, microzones of filter paper, printed by a wax printing method, were modified with amino-functionalized SBA-15 and subsequently, anti-EE2 specific antibodies were covalently immobilized.The determination of EE2 in water was carried out by adding a fixed concentration of EE2 conjugated with the enzyme horseradish peroxidase (HRP) to samples and standards. Then, the FPAD were added and incubated for 10 min. Finally, the detection was performed by the reaction of 10-acetyl-3,7-dihydroxyphenoxazine (ADHP) whose oxidation is catalyzed by HRP in the presence of H2O2, obtaining the highly fluorescent resorufin (R). Resorufin was detected by LED excitation at 550 nm, observing emission at 585 nm. The EE2 concentration in the samples was inversely proportional to the relative fluorescence obtained from the enzymatic reaction products. The FPAD assay showed a detection limit (LOD) of 0.05 ng L -1 and coefficients of variation (CV) below 4.5 % within-assay and below 6.5 % between-assay, respectively. The results obtained show the potential suitability of our FPAD for the selective and sensitive quantification of EE2 in river water samples. In addition, it has the PADs advantages of being disposable, easy to apply and inexpensive.

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Section: Accepted Manuscriptmentioning

confidence: 99%

Ethinylestradiol quantification in drinking water sources using a fluorescent paper based immunosensor

Scala-Benuzzi

Takara

Alderete

et al. 2018

Microchemical Journal

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“…Electrochemical immunosensors are a new class of sensors that combine highly sensitive electrochemical sensing technology with highly specific immunological techniques. − Based on the specific binding of the antigen and antibody, the electrochemical immunosensor analysis can detect the change of the antigen–antibody conjugate and convert the nonelectric signal into an electrical signal , so as to realize the correlation between the electric signal and the concentration of the substance to be tested, which is a relatively advanced detection method and has broad application prospects in food, environment, and other fields. − Poo-arporn et al prepared a disposable electrochemical sensor for determination of RAC with a detection limit of 13 nM (S/N = 3) . A disposable electroanalytical device for the competitive enzyme-linked immunosorbent assay of phenylethanolamine A (PA) was reported by Deng et al with a detection range of 0.005–60 ng·mL –1 and a detection limit of 2.6 pg·mL –1 . Kang et al developed a facile and sensitive method for the detection of CL using a personal glucose meter, suggesting a good linear correlation with the logarithm of the CL concentrations in the range of 0.1–100 ng·mL –1 .…”

Section: Introductionmentioning

confidence: 99%

Detection of Six β-Agonists by Three Multiresidue Immunosensors Based on an Anti-bovine Serum Albumin-Ractopamine-Clenbuterol-Salbutamol Antibody

Ren

Zhang

et al. 2020

ACS Omega

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According to an indirect competitive immunoassay, six β-agonists (clenbuterol (CL), salbutamol (SAL), ractopamine (RAC), terbutaline (TER), mabuterol (MAB), and tulobuterol (TUL)) were detected by three novel multiresidue immunosensors on the basis of the successful preparation of bovine serum albumin (BSA)-RAC-CL-SAL multideterminant antigen and anti-BSA-RAC-CL-SAL antibody. A new strategy was reported to detect six β-agonists by combining nanotechnology, electrochemical detection, and specific immune technology. At the same concentration, the amperometric response for detection of six β-agonists was in a sequence of GCE/GNP/SAL > GCE/GNP/RAC > GCE/GNP/CL. Detection limits of six β-agonists show that the multiresidue detection performance of the GCE/GNP/RAC immunosensor is better than those of GCE/GNP/SAL and GCE/GNP/CL immunosensors. Three immunosensors manifest superior properties with a wide linear range, low detection limit, excellent reproducibility, and stability. The proposed GCE/GNP/RAC immunosensor displays high accuracy and can be effectively used for real sample detection.

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“…Currently, the methods used to determine PEA concentrations include high-performance liquid chromatography (HPLC), liquid chromatography-tandem mass spectrometry (LC-MS/MS) , gas chromatography-mass spectrometry (GC-MS), and liquid chromatography-mass spectrometry (LC-MS), as well as electrochemical (Liu, Cai, et al, 2017) and immunological methods (Mei et al, 2014). Although instrumental detection methods are characteristically highly accurate (Tang et al, 2015), the instruments are expensive, the preprocessing methods and operations are complex, and the times required for detection are long.…”

Section: Introductionmentioning

confidence: 99%

Immunochromatographic strip for rapid detection of phenylethanolamine A

Jiang

Zeng

Liu

et al. 2017

Food and Agricultural Immunology

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A monoclonal antibody (mAb) directed against phenylethanolamine A (PEA) was successfully prepared with the immunization of mice and cell fusion, and used to develop a detection method for a colloidal gold immunoassay. The 50% inhibitory concentration of mAb PEA-2G10 was 0.213 ng/mL, the limit of detection was 0.033 ng/mL, and its detection range 0.033-0.679 ng/mL. The recovery rate for PEA in swine urine was 84.5-90.8%, with good stability and accuracy. "Dry" immunoassay test strips were successfully prepared based on antibody 2G10. When the strip was tested in swine urine, the cutoff value for PEA was 5 ng/mL, with highly sensitivity. The method developed here can be used to test swine urine on-site for the rapid detection of PEA.ARTICLE HISTORY

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Multichannel electroanalytical devices for competitive ELISA of phenylethanolamine A

Cited by 14 publications

References 51 publications

Ethinylestradiol quantification in drinking water sources using a fluorescent paper based immunosensor

Ethinylestradiol quantification in drinking water sources using a fluorescent paper based immunosensor

Detection of Six β-Agonists by Three Multiresidue Immunosensors Based on an Anti-bovine Serum Albumin-Ractopamine-Clenbuterol-Salbutamol Antibody

Immunochromatographic strip for rapid detection of phenylethanolamine A

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