Multicentre performance evaluation of the E170 Module for MODULAR ANALYTICS

Bieglmayer, Christian; Chan, Daniel W.; Sokoll, Lori J.; Imdahl, Roland; Kobayashi, Masaji; Yamada, Erike; Lilje, Diana J.; Luthe, Hilmar; Meissner, J; Messeri, Gianni; Celli, Alessandra; Tozzi, Paola; Roth, Heinz Jürgen; Schmidt, Frank Peter; Mächler, Marie Luise; Schuff‐Werner, P.; Zingler, Christiana; Smitz, Johan; Schiettecatte, Johan; Vonderschmitt, Dieter J.; Pei, P.; Ng, Katherine; Ebert, Christoph; Kirch, Peter; Wanger, Michael; McGovern, Margaret M.; Stockmann, Wolfgang; Kunst, Albert

doi:10.1515/cclm.2004.239

Cited by 16 publications

(13 citation statements)

References 3 publications

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“…The reaction mixture is aspirated into the measuring cell where the microparticles are magnetically captured onto the surface of the electrode. Unbound substances are removed by a washing step, and application of voltage to the electrode induces chemiluminescent emission, which is measured by a photomultiplier 15. According to the competitive assay design, an inverse proportional calibration curve is obtained.…”

Section: Methodsmentioning

confidence: 99%

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Multicenter Analytical Evaluation of the Automated Electrochemiluminescence Immunoassay for Cyclosporine

Vogeser¹,

Shipkova

Rigo-Bonnin

et al. 2014

Therapeutic Drug Monitoring

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Section: Methodsmentioning

confidence: 99%

“…Pretreated samples are combined with biotinylated analyte-specific antibodies and labeled analyte derivatives and recovered with streptavidin microparticles 15. Detection is by electrochemiluminescence (see Materials and Methods).…”

Section: Methodsmentioning

confidence: 99%

Multicenter Analytical Evaluation of the Automated Electrochemiluminescence Immunoassay for Cyclosporine

Vogeser¹,

Shipkova

Rigo-Bonnin

et al. 2014

Therapeutic Drug Monitoring

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“…Nevertheless, this was unlikely to have influenced results because the two methods yield comparable results. 26 A third limitation was the use of plasma cortisol selectivity ratios as the reference standard. All AVS selectivity indices used in both research and clinical practice are arbitrary because they have not been formally linked to outcome data in an evidence-based manner.…”

Section: Hypertensionmentioning

confidence: 99%

Plasma Metanephrine for Assessing the Selectivity of Adrenal Venous Sampling

et al. 2013

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A drenal hypertension caused by primary aldosteronism comprises the most common curable form of secondary hypertension. In the analytic workup of patients with primary aldosteronism, adrenal venous sampling (AVS) is recommended for establishing the origins of excess production of hormones.1 AVS is a technically demanding procedure in which correct cannulation of the adrenal veins, especially the right, can pose significant difficulty. 2,3 Correct positioning of the catheter is verified by measurement of plasma cortisol concentrations. High cortisol concentrations in adrenal blood compared with peripheral blood ascertain correct catheter placement and thus selective sampling. Because cortisol has a long circulating halflife (100 minutes), increases in adrenal vein (AV) blood above levels of peripheral venous (PV) blood are relatively minor and subsequently subject to interpretative error. Furthermore, as a result of physiological corticotropin fluctuations, cortisol is secreted in a variable fashion so that fluctuating levels can interfere with the interpretation of AVS selectivity. [4][5][6] This problem can be overcome using cosyntropin stimulation. 7 Cosyntropin stimulation, however, adds to the complexity of the procedure and for this reason is not always used.With the above considerations in mind, there seems a need for more reliable parameters than cortisol in assessing the correct positioning of catheters during AVS.8 Plasma metanephrine, the O-methylated metabolite of epinephrine, represents one such alternative analyte. More than 90% of plasma metanephrine is produced within the adrenal medulla, with <10% produced from epinephrine after release from the adrenals. 7 Compared with cortisol, plasma metanephrine has a short circulating half-life of 3 to 6 minutes, resulting in close to 90-fold increases of AV compared with PV concentrations in situations where catheters are correctly positioned.7 Such large Abstract-Adrenal vein sampling is used to establish the origins of excess production of adrenal hormones in primary aldosteronism. Correct catheter positioning is confirmed using adrenal vein measurements of cortisol, but this parameter is not always reliable. Plasma metanephrine represents an alternative parameter. The objective of our study was to determine the use of plasma metanephrine concentrations to establish correct catheter positioning during adrenal vein sampling with and without cosyntropin stimulation. We included 52 cosyntropin-stimulated and 34 nonstimulated sequential procedures. Plasma cortisol and metanephrine concentrations were measured in adrenal and peripheral venous samples. Success rates of sampling, using an adrenal to peripheral cortisol selectivity index of 3.0, were compared with success rates of metanephrine using a selectivity index determined by receiver operating characteristic curve analysis. Among procedures assessed as selective using cortisol, the adrenal to peripheral vein ratio of metanephrine was 6-fold higher than that of cortisol (94.0 versus 15.5; P<0.0001). Ther...

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“…Undiluted 100-l serum samples were eluted at 0.25 ml͞min with 10 mM Tris-buffered 140 mM NaCl containing 2 mM CaCl 2 , pH 8.0, monitored at 280 nm, and 250-l fractions were collected for CRP and cholesterol assay. Serum concentrations of estradiol and testosterone were measured by electrochemiluminescence immunoassay (Roche Diagnostics) (35) in the bleedout samples from the 56-week cohort.…”

mentioning

confidence: 99%

Transgenic human C-reactive protein is not proatherogenic in apolipoprotein E-deficient mice

Hirschfield

Gallimore

Kahan

et al. 2005

Proc. Natl. Acad. Sci. U.S.A.

178

123

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The association between circulating concentrations of C-reactive protein (CRP) and future atherothrombotic events has provoked speculation about a possible pathogenetic role of CRP. However, we show here that transgenic expression of human CRP had no effect on development, progression, or severity of spontaneous atherosclerosis, or on morbidity or mortality, in male apolipoprotein E (apoE)-deficient C57BL͞6 mice up to 56 weeks, despite deposition of human CRP and mouse complement component 3 in the plaques. Although female apoE knockouts develop atherosclerosis more rapidly than males, the human CRP transgene is under sex hormone control and is expressed at human levels only in males. We therefore studied only male mice. The concentration of mouse serum amyloid P component, an extremely sensitive systemic marker of inflammation, remained normal throughout except for transient spikes in response to fighting in a few animals, indicating that atherogenesis in this model is not associated with an acute-phase response. However, among human CRP transgenic mice, the circulating CRP concentration was higher in apoE knockouts than in wild-type controls. The higher CRP values were associated with substantially lower estradiol concentrations in the apoE-deficient animals. Human CRP transgene expression is thus up-regulated in apoE-deficient mice, apparently reflecting altered estrogen levels, despite the absence of other systemic signs of inflammation. Extrapolation to human pathology from this xenogeneic combination of human CRP with apoE deficiency-mediated mouse atherosclerosis must be guarded. Nevertheless, the present results do not suggest that human CRP is either proatherogenic or atheroprotective in vivo.atherosclerosis ͉ inflammation ͉ transgenic

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Multicentre performance evaluation of the E170 Module for MODULAR ANALYTICS

Cited by 16 publications

References 3 publications

Multicenter Analytical Evaluation of the Automated Electrochemiluminescence Immunoassay for Cyclosporine

Multicenter Analytical Evaluation of the Automated Electrochemiluminescence Immunoassay for Cyclosporine

Plasma Metanephrine for Assessing the Selectivity of Adrenal Venous Sampling

Transgenic human C-reactive protein is not proatherogenic in apolipoprotein E-deficient mice

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