Multicenter International Work Flow Study of an Automated Polymerase Chain Reaction Instrument

Klapper, Paul E.; Jungkind, Donald; Fenner, Thomas; Antinozzi, R.; Schirm, J; Blanckmeister, Carolyn A.

doi:10.1093/clinchem/44.8.1737

Cited by 23 publications

(4 citation statements)

References 12 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Their software Polara TM enables an easy set‐up and scheduling of the procedures and controls the peripherals, either directly or through their own software, hence freeing the procedures from any human intervention. As it is often the major cost, a decrease in hands‐on will also decrease the cost of the analyses (Klapper et al. 1998).…”

Section: The Sw Experience In Automating Dna Marker Analysismentioning

confidence: 99%

Automation of DNA marker analysis for molecular breeding in crops: practical experience of a plant breeding company

Dayteg¹,

Tuvesson²,

Merker

et al. 2007

Plant Breeding

View full text Add to dashboard Cite

In modern plant breeding, DNA marker analyses are of increasing importance and, as the methods become more widely adopted, the capacity for high-throughput analyses at low cost is crucial for its practical use. Automation of the analysis processes is a way to meet these requirements. In order to achieve this, while keeping adequate flexibility in the analysis processes, Svalo¨f Weibull AB (SW) has developed a fully automated polymerase chain reaction system. It has been evaluated on barley and canola lines and is capable of analysing up to 2200 samples per day at a cost of 0,24 € per analysis for markerassisted selection and quality control of genetically modified organisms. A detailed description of this system is given, and improvements to the throughput and applications are discussed.

show abstract

Section: The Sw Experience In Automating Dna Marker Analysismentioning

confidence: 99%

Automation of DNA marker analysis for molecular breeding in crops: practical experience of a plant breeding company

Dayteg¹,

Tuvesson²,

Merker

et al. 2007

Plant Breeding

View full text Add to dashboard Cite

show abstract

“…[13][14][15] Although PCR has high sensitivity, this method is still faced with many instinctive limitations owing to the need for professional technicians, harsh experimental conditions and a long turnaround time, which has seriously restricted their application in lowresource settings. [16][17][18] Furthermore, false-positive results may be obtained because the thermal cycling amplification step has a risk of erroneous nonspecific amplification of contaminants. [19][20][21] To resolve these issues, various methods have been utilized to detect gene sequences.…”

Section: Introductionmentioning

confidence: 99%

Highly sensitive detection of prostate cancer specific PCA3 mimic DNA using SERS-based competitive lateral flow assay

Wen

et al. 2019

Nanoscale

View full text Add to dashboard Cite

show abstract

“…During the past decade, advances in PCR technology and other DNA signal and target amplification techniques have resulted in these molecular diagnostics becoming key procedures (4,107,117). Such techniques are conceptually simple, highly specific, sensitive, and amenable to full automation (54,115). The most mature of these technologies, PCR, is in one variant or another now common in research laboratories and is used increasingly in routine diagnostic laboratory settings and undergraduate and high-school teaching (32,38,40,101).…”

Section: Introductionmentioning

confidence: 99%

Multiplex PCR: Optimization and Application in Diagnostic Virology

et al. 2000

View full text Add to dashboard Cite

SUMMARY PCR has revolutionized the field of infectious disease diagnosis. To overcome the inherent disadvantage of cost and to improve the diagnostic capacity of the test, multiplex PCR, a variant of the test in which more than one target sequence is amplified using more than one pair of primers, has been developed. Multiplex PCRs to detect viral, bacterial, and/or other infectious agents in one reaction tube have been described. Early studies highlighted the obstacles that can jeopardize the production of sensitive and specific multiplex assays, but more recent studies have provided systematic protocols and technical improvements for simple test design. The most useful of these are the empirical choice of oligonucleotide primers and the use of hot start-based PCR methodology. These advances along with others to enhance sensitivity and specificity and to facilitate automation have resulted in the appearance of numerous publications regarding the application of multiplex PCR in the diagnosis of infectious agents, especially those which target viral nucleic acids. This article reviews the principles, optimization, and application of multiplex PCR for the detection of viruses of clinical and epidemiological importance.

show abstract

Multicenter International Work Flow Study of an Automated Polymerase Chain Reaction Instrument

Cited by 23 publications

References 12 publications

Automation of DNA marker analysis for molecular breeding in crops: practical experience of a plant breeding company

Automation of DNA marker analysis for molecular breeding in crops: practical experience of a plant breeding company

Highly sensitive detection of prostate cancer specific PCA3 mimic DNA using SERS-based competitive lateral flow assay

Multiplex PCR: Optimization and Application in Diagnostic Virology

Contact Info

Product

Resources

About