Multicenter comparison of the new Cobas 6800 system with Cobas Ampliprep/Cobas TaqMan and Abbott RealTime for the quantification of HIV, HBV and HCV viral load

Wirden, Marc; Larrouy, Lucile; Mahjoub, Nadia; Todesco, Eve; Damond, Florence; Delagrèverie, Héloïse; Akhavan, Sepideh; Charpentier, Charlotte; Chaix, Marie‐Laure; Descamps, Diane; Cálvez, Vincent; Marcelin, Anne‐Geneviève

doi:10.1016/j.jcv.2017.09.007

Cited by 27 publications

(19 citation statements)

References 20 publications

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“…performance. To date, few published studies have compared the analytical performance of different next-generation assay platforms with higher levels of automation used for the measurement of VL, only four of which included c6800 [29,31,32,49], and only one with c6800 and Aptima results [49].…”

Section: Discussionmentioning

confidence: 99%

“…VL measurements may be performed at different laboratories during long-term disease management, and variable assay performance may hamper the concordance and commutability of results and may lead to inappropriate treatment [26][27][28][29][30]. Commonly used VL tests include those manufactured by Roche (cobas 6800/8800; c6800) and cobas 4800 (c4800) systems [29,[31][32][33], Abbott (m2000 RealTime) [34,35], Hologic (Aptima, Panther) [36][37][38][39][40], Beckman (VERIS) [41][42][43][44][45][46][47][48] and others. Most of these assays rely on quantitative, real-time RT-PCR, while the Aptima assay uses transcription-mediated amplification (TMA), which may impart some differences in performance characteristics.…”

Section: Introductionmentioning

confidence: 99%

“…Most of these assays rely on quantitative, real-time RT-PCR, while the Aptima assay uses transcription-mediated amplification (TMA), which may impart some differences in performance characteristics. While several recent studies have demonstrated concordance among most HIV-1, HBV, and HCV VL assays, there are few data comparing assays that are available on highthroughput molecular platforms such as c6800 and Panther [32,49].…”

Section: Introductionmentioning

confidence: 99%

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Analytical performance of four molecular platforms used for HIV-1, HBV and HCV viral load determinations

Aretzweiler

Leuchter

García–Álvarez

et al. 2019

Expert Review of Molecular Diagnostics

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Background: Viral load (VL) quantification is important for the management of HBV, HCV, and HIV-1-infected patients. Several semi-or fully automated systems and assays are available that can be used to measure VL for these and other targets. Research design and methods: We assessed the accuracy, genotype/subtype inclusivity, and precision of four VL assays for three viral targets: cobas 4800 (Roche), cobas 6800 (Roche), Aptima (Hologic) and VERIS (Beckman), using WHO standards, cell culture supernatants and clinical samples. Results: Most results were close to expected values, except for significant under-quantification of HIV-1 group O, HBV genotype C, and D at high VL, and HCV genotype 3 by Aptima, and of HIV-1 CRF01_AE and group N and HCV genotype 3 by VERIS. Precision was comparable between tests except for VERIS HCV, which showed more variability. Aptima and cobas 6800 results agreed well with each other except HBV VL at lower VL (<10,000 IU/mL) where Aptima results tended to be higher. Conclusions: Results from different VL assays may not always agree in certain subsets of patients. Clinicians should we aware of these findings when making treatment decisions.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Analytical performance of four molecular platforms used for HIV-1, HBV and HCV viral load determinations

Aretzweiler

Leuchter

García–Álvarez

et al. 2019

Expert Review of Molecular Diagnostics

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“…Multiple target, automated molecular testing instruments are manufactured by Roche (cobas 6800/8800 and cobas 4800 systems) [3][4][5][6], Abbott (m2000 RealTime and Alinity m) [7,8], Beckton-Dickinson (Viper, BD MAX) [9][10][11][12][13], Cepheid (Infinity80) [13,14] and Hologic (Aptima, Panther) [15][16][17][18][19]. Apart from Aptima assays on the Panther system, these systems rely on quantitative, real-time PCR to detect and/or quantitate various target analytes.…”

Section: Introductionmentioning

confidence: 99%

Generating timely molecular diagnostic test results: workflow comparison of the cobas® 6800/8800 to Panther

Aretzweiler

Leuchter

Simon

et al. 2019

Expert Review of Molecular Diagnostics

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Background: Molecular diagnostic tests for HBV, HCV and HIV-1 and other pathogens are widely used for clinical management. Practical issues related to workflow and labor requirements need to be characterized to inform selection of the most appropriate system. Research design and methods: We compared the workflow of two high-throughput systems: cobas 6800 (Roche) and Panther (Hologic), using average mid-size laboratory test volumes for five different assays (HIV-1, HBV, HCV, HPV or TV, and CT/NG). Results: Set-up time, time to first results, time to last results, and total hands-on time for cobas 6800 was 0.40, 2.47, 7.12, and 0.98 hours, respectively; on the Panther system, these times were 0.75, 2.7, 9.1, and 1.48 hours. Fifty-seven samples had results available at the first time point on cobas 6800 compared to 5 samples on the Panther system. The Panther system required more manual steps including several with potential risks of contamination or error. The number of reagents items required was 5 for cobas 6800 and 40 for the Panther system. Conclusions: Both systems provided a high level of automation. The cobas 6800 platform had shorter start up, time to first result, time to last result and hands-on times than the Panther system. ARTICLE HISTORY

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“…There are multiple large scale, high‐throughput molecular testing instruments available 12 . The best known include the Roche 6800/8800, NeuMoDx Molecular Instrument, Luminex NxTAG, and Hologic Panther Fusion Instruments 13‐16 . These are larger platforms that can process and test between several hundred and several thousand tests per day.…”

Section: Introductionmentioning

confidence: 99%

An evolving approach to the laboratory assessment of COVID‐19

Stratton

Tang

2020

Journal of Medical Virology

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Multicenter comparison of the new Cobas 6800 system with Cobas Ampliprep/Cobas TaqMan and Abbott RealTime for the quantification of HIV, HBV and HCV viral load

Cited by 27 publications

References 20 publications

Analytical performance of four molecular platforms used for HIV-1, HBV and HCV viral load determinations

Analytical performance of four molecular platforms used for HIV-1, HBV and HCV viral load determinations

Generating timely molecular diagnostic test results: workflow comparison of the cobas® 6800/8800 to Panther

An evolving approach to the laboratory assessment of COVID‐19

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