Multicenter comparison of the high volume (10 ml) NR bactec plus system and the standard (5 ml) NR bactec system

Koontz, Franklin P.; Flint, Kristine K.; Reynolds, Janet K.; Allen, Stephen D.

doi:10.1016/0732-8893(91)90044-g

Cited by 32 publications

(23 citation statements)

References 12 publications

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“…The reason for the reported higher detection speed in BACTEC" systems could perhaps be a higher sensitivity when using a more unbiased automated C0,-detection principle compared with a more or less person-dependent visual examination principle in conventional systems. However, especially the resin-containing BAC-TECm media which were included in our study have been claimed to be of great value in the detection of bacteremia, especially in patients who are receiving antibiotics at the time the blood is collected (7,13). Other investigators have been more sceptical concerning the value of antibiotic removal devices in patients treated with antibiotics (11,15,24).…”

Section: Discussionmentioning

confidence: 96%

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Comparison of detection speed and yield in BACTEC NR-860® and Roche BCB® blood culture systems

Arpi

Bremmelgaard

1993

Apmis

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Detection speed and yield were compared between BACTEC NR‐860® and Roche BCB® blood culture systems. From a total of 1,550 paired blood cultures inoculated with the same volume of blood, 161 (10.4%) grew 180 organisms from 140 adult patients. Sixty‐six percent of the isolates were clinically significant. The BACTEC® system detected more clinically significant isolates than the BCB® system, 91% vs 76% (p < 0.01), especially Enterobacteriaceae. In 39 paired blood cultures, 42 significant organisms were detected at different times in the two systems. The vast majority (93%) were detected first (on average 1 day faster) by the BACTEC® system (p < 0.05), especially Escherichia coli and streptococci. Generally, detection times were shorter in the BACTEC® system. Three fourths (76%) of all significant organisms were detected within the first incubation day as compared to less than half (42%) in the BCB® system (p < 0.001). Generally, the agitated aerobic resin medium BACTEC 26 Plus® had the fastest detection and highest yield in our study. The contamination rate, below 2% in both systems, was acceptable. The BACTEC NR‐860® system with resin‐containing media proved to be a reliable, sensitive and fast blood culture system, which deserves further investigation.

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Section: Discussionmentioning

confidence: 96%

“…It has been reported that resins have effects other than removal of antibiotics that could result in increased microbial recovery and enhanced detection (12,13). The mechanical action of resins in agitated media resulted in a total lysis of different blood cells and disrupted chains and clumps of microorganisms.…”

Section: Discussionmentioning

confidence: 99%

Comparison of detection speed and yield in BACTEC NR-860® and Roche BCB® blood culture systems

Arpi

Bremmelgaard

1993

Apmis

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“…This high vacuum can easily result in overfilling the blood culture bottles, thereby exceeding the recommended blood:broth (B:b) ratio [21]. The literature describes a decline of the time to positivity of true-positive bottles [22] and an increase in false-positives of overfilled bottles of up to 0.9 % [23,24]. Because of the excess of natural inhibiting factors present in blood, false-negative cultures are to be expected [25].…”

Section: Discussionmentioning

confidence: 99%

Evaluation of the BD BACTEC FX blood volume monitoring system as a continuous quality improvement measure

Coorevits¹,

Abeele²

2015

Eur J Clin Microbiol Infect Dis

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The yield of blood cultures is proportional to the volume of blood cultured. We evaluated an automatic blood volume monitoring system, recently developed by Becton Dickinson within its BACTEC EpiCenter module, that calculates mean volumes of negative aerobic bottles and generates boxplots and histograms. First, we evaluated the filling degree of 339 aerobic glass blood cultures by calculating the weight-based volume for each bottle. A substantial amount of the bottles (48.3%) were inadequately filled. Evaluation of the accuracy of the monitoring system showed a mean bias of -1.4 mL (-15.4%). Additional evaluation, using the amended software on 287 aerobic blood culture bottles, resulted in an acceptable mean deviation of -0.3 mL (-3.3%). The new software version was also tested on 200 of the recently introduced plastic bottles, which will replace the glass bottles in the near future, showing a mean deviation of +2.8 mL (+26.7%). In conclusion, the mean calculated volumes can be used for the training of a single phlebotomist. However, filling problems appear to be masked when using them for phlebotomist groups or on wards. Here, visual interpretation of boxplots and histograms can serve as a useful tool to observe the spread of the filling degrees and to develop a continuous improvement program. Re-adjustment of the software has proven to be necessary for use with plastic bottles. Due to our findings, BD has developed further adjustments to the software for validated use with plastic bottles, which will be released soon.

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“…The yield of blood cultures from adults is clearly volume-dependent [1][2][3][4][5][6][7][8][9] and increases approximately 3% for each additional millilitre of blood obtained [3][4] . We found an increased yield of approximately 3.3% per millilitre in our study.…”

Section: Discussionmentioning

confidence: 99%

“…The volume of blood drawn in adult patients is the most important single factor governing the sensitivity of blood cultures [1][2][3][4][5][6][7][8][9] , and 10 to 20 ml of blood per draw has been recommended [4,6,[10][11][12] .…”

Section: Introductionmentioning

confidence: 99%

Detection of Bacteraemia in an Inadequate Volume of Blood

Siddiqui¹,

Eltahawy²,

Bahnassy³

2000

med

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ABSTRACT. It was recently discovered that 15% of adult blood culture specimens at King Abdulaziz University Hospital were inadequate in volume where only 2 ml were collected instead of the required 8 ml using the Signal oxoid medium. A comparison of 263 matched pairs of standard-volume (8 ml) and low-volume (2 ml) blood cultures from adult patients in the medical ward and intensive care unit at King Abdulaziz University Hospital showed that standard-volume cultures had a higher detection rate for bloodstream infection than did low-volume cultures (89% compared with 69%; difference: 20%, P = 0.01). Our data show that the yield of blood cultures in adults increases approximately 3.3% per millilitre of blood cultured, and that the sensitivity of the standardvolume cultures is statistically higher than the sensitivity of the low-volume cultures (84% vs. 65%, P < 0.01).

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Multicenter comparison of the high volume (10 ml) NR bactec plus system and the standard (5 ml) NR bactec system

Cited by 32 publications

References 12 publications

Comparison of detection speed and yield in BACTEC NR-860® and Roche BCB® blood culture systems

Comparison of detection speed and yield in BACTEC NR-860® and Roche BCB® blood culture systems

Evaluation of the BD BACTEC FX blood volume monitoring system as a continuous quality improvement measure

Detection of Bacteraemia in an Inadequate Volume of Blood

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