Multicenter clinical comparison of resincontaining bottles with standard aerobic and Anaerobic bottles for culture of microorganisms from blood

Abstract

In a study comparing the Bactec 9240 (Plus Aerobic/F and Anaerobic/F bottles, containing resins; Becton Dickinson, USA) and the Vital (standard aerobic and anaerobic bottles, with no additives; bioMérieux, France) blood culture systems, 6456 sets of four bottles of 9660 blood cultures submitted were evaluated. There were 531 clinically significant isolates from 795 positive blood cultures. Of the 531 positive blood cultures, 355 were positive in both systems, 141 with the Bactec 9240 alone, and 30 with the Vital alone (p < 0.001); five were not detected by either system. The average time to detection of positive cultures for the matched sets was 10.65 h and 18.41 h by the Bactec 9240 system and the Vital system, respectively. The false-positive rate per bottle was 0.65% in the Bactec 9240 and 0.71% in the Vital. The rate of false-negative pairs (i.e., major errors) was very low (0.12% for the Bactec 9240, 0.19% for the Vital) and not significantly different between the two systems. The striking differences in recovery of microorganisms may be due to the presence of resins in the Bactec medium. However, the observed superiority of the Bactec 9240, even for patients not receiving antibiotics, suggests that resins adsorb other inhibitors present in patients' blood.

Controlled Clinical Comparison of BACTEC Plus Anaerobic/F to Standard Anaerobic/F as the Anaerobic Companion Bottle to Plus Aerobic/F Medium for Culturing Blood from Adults

“…The superior recovery from Plus Anaerobic/F bottles parallels that reported for other blood culture bottles containing resins or similar additives (2,9,10,18,22,24). In controlled clinical trials these additives have been shown to improve microbial recovery, particularly for staphylococci (10,20,24,25,26,28,29).…”

“…In controlled clinical trials these additives have been shown to improve microbial recovery, particularly for staphylococci (10,20,24,25,26,28,29). The mechanism(s) by which these additives increase microbial recovery is unknown.…”

“…In the study described here, we compared the BACTEC Plus Anaerobic/F bottle with the Standard Anaerobic/F bottle for recovery of bacteria and fungi from adult patients. The two anaerobic bottles were paired with the Plus Aerobic/F bottle, as that bottle has previously been shown to recover pathogenic microorganisms with yields superior to those from non-resin-containing bottles (2,10,24) and equivalent to those from other high-volume resin-containing bottles (9,18,22).…”

Optimum Detection Times for Bacteria and Yeast Species with the BACTEC 9120 Aerobic Blood Culture System: Evaluation for a 5-Year Period in a Turkish University Hospital

“…The false-negative rate was found as 0.1% in our study. The false-negative rate was reported to be 0.12% by using BACTEC 9240 in the research of Lelievre et al (7). The low rate of false positives and negatives in our laboratory, together with the routine confirmation of results by microscopy and cultures, prevents many of the complications that may arise in practice.…”

Evaluation of Bactec Mycosis IC/F and Plus Aerobic/F Blood Culture Bottles for Detection of Candida in the Presence of Antifungal Agents

“…Bactec PAF bottles were chosen for comparison with MICF bottles, because they contain a standard medium for the detection of bacterial and fungal pathogens not specifically adapted to fungi and include resins, which can bind and inactivate antifungal agents and other drugs. Several investigations demonstrated that Bactec PAF bottles yielded better results for detecting bacteremia in patients undergoing pharmacotherapy, indicating that the resins have an effect on the inactivation of potential inhibitors of microbiological pathogen growth (20,21).…”