Multiarmed star-like platinum nanowires with multienzyme assembly for direct electronic determination of carcinoembryoninc antigen in serum

Self-enhanced electrochemiluminescence immunosensor based on nanowires obtained by a green approach

“…However, the ECL signal was greatly improved after the modification of to 80 ng mL -1 with a relatively low detection limit (defined as LOD = 3S B /m, where m is the slope of the corresponding calibration curve and S B is the standard deviation of the blank (Radi et al, 2006;Centi et al, 2007)) of 0.3 pg mL -1 . Moreover, Table S1 showed the linear range and detection limit of immunosensors with previous reports (Song et al, 2011;Su et al, 2011;Zhang et al, 2012;Deng et al, 2011). Compared to other methods, the immunosensor had a relative wide linear range and low detection limit.…”

Irregular-shaped platinum nanoparticles as peroxidase mimics for highly efficient colorimetric immunoassay

“…When anti-RIgG antibodies were immobilized onto the ISPtNP, the absorption bands for amide I and amide II could be obviously achieved (curve 'c'). The results revealed that anti-RIgG antibodies could be conjugated onto the ISPtNP through the formation of the Pt S bond or Pt NH bond due to the interaction between cysteine or NH 3 + -lysine residues of anti-RIgG antibodies and the ISPtNP [21,[34][35][36][37].…”

“…One of the major problems associated with covalent binding is the bioactive decrease when the biomolecules are exposed to reactive groups and harsh reaction conditions [17]. Noble metals including Pt and Au nanoparticles are often used for the labeling of biomolecules owing to the strong interaction between proteins and nanoparticles [18][19][20][21].…”

Electrochemical lectin-based biosensor array for detection and discrimination of carcinoembryonic antigen using dual amplification of gold nanoparticles and horseradish peroxidase

“…Extensive efforts have been devoted to the determination of CEA based on the various methods such as thermal lens microscope [12], fluorescence immunoassay [13], chemiluminescence immunoassay [14], surface-enhanced Raman scattering immunoassay [15] and electrochemical immunoassay [16][17][18][19][20][21][22][23][24][25][26] and so forth. Among them, electrochemical immunosensors have recently gained increasing attention in the quantitative detection of CEA owing to low cost, fast response, and ease of handling and miniaturization.…”