Multianalyte assay system developed for drugs of abuse

Parsons, Robert G.; Kowal, R; LeBlond, David; Yue, Vincent; Neargarder, L; Bond, L; Garcia, Diana Gonzalez; Slater, Dick; Rogers, Philip J.

doi:10.1093/clinchem/39.9.1899

Cited by 28 publications

(5 citation statements)

References 0 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…There are several recent reports describing multianalyte immunosensors. Many describe a method for multianalyte sensing but demonstrate the detection of only a single analyte. − Others combine discrete sensing substrates, such as optical fibers, capillaries, microtiter wells, or dipsticks, to analyze for multiple analytes simultaneously. Another general approach employs different capture molecules in different regions of a sensing substrate and detects binding using a label-free approach. , These label-free methods continue to be susceptible to problems such as low sensitivity and increased backgrounds due to nonspecific binding.…”

mentioning

confidence: 99%

Array Biosensor for Simultaneous Identification of Bacterial, Viral, and Protein Analytes

et al. 1999

View full text Add to dashboard Cite

The array biosensor was fabricated to analyze multiple samples simultaneously for multiple analytes. The sensor utilized a standard sandwich immunoassay format: Antigen-specific "capture" antibodies were immobilized in a patterned array on the surface of a planar waveguide and bound analyte was subsequently detected using fluorescent tracer antibodies. This study describes the analysis of 126 blind samples for the presence of three distinct classes of analytes. To address potential complications arising from using a mixture of tracer antibodies in the multianalyte assay, three single-analyte assays were run in parallel with a multianalyte assay. Mixtures of analytes were also assayed to demonstrate the sensor's ability to detect more than a single species at a time. The array sensor was capable of detecting viral, bacterial, and protein analytes using a facile 14-min assay with sensitivity levels approaching those of standard ELISA methods. Limits of detection for Bacillus globigii, MS2 bacteriophage, and staphylococcal enterotoxin B (SEB) were 10(5) cfu/mL, 10(7) pfu/mL, and 10 ng/mL, respectively. The array biosensor also analyzed multiple samples simultaneously and detected mixtures of the different types of analytes in the multianalyte format.

show abstract

mentioning

confidence: 99%

Array Biosensor for Simultaneous Identification of Bacterial, Viral, and Protein Analytes

et al. 1999

View full text Add to dashboard Cite

show abstract

“…The second strategy, proposed by Ekins and his colleagues, is based on the spatial resolution of different bioactive species on the same substrate that creates an array, permitting the detection of all the analytes through the same universal tracer. Due to the simplicity it offers, this approach seems to be well suited for the development of both multianalyte immunoassays − and immunosensors. − …”

mentioning

confidence: 99%

Simultaneous Determination of Pesticides Using a Four-Band Disposable Optical Capillary Immunosensor

et al. 2002

View full text Add to dashboard Cite

The development of a four-band capillary optical immunosensor for the simultaneous determination of mesotrione, hexaconazole, paraquat, and diquat is described. Four distinct bands (each corresponding to a different analyte) are created in the internal walls of a plastic capillary by immobilizing protein conjugates of the analytes. To perform the assay, the capillary is filled with a mixture of anti-analyte-specific antibodies together with a standard or sample containing the analyte(s). After a short incubation, a mixture of the appropriate second antibodies labeled with fluorescein is introduced into the capillary. To measure the fluorescence intensity bound onto each band, the capillary was scanned, perpendicularly to its axis, by a laser light beam. Part of the emitted photons were trapped into the capillary walls and waveguided to a photomultiplier placed at the one end of the capillary. The analytical characteristics of the assays of mesotrione, paraquat, diquat, and hexaconazole were as follows: detection limits of 0.04, 0.06, 0.09, and 0.10 ng/mL, respectively; dynamic ranges up to 9, 6, 12, and 15 ng/ mL, respectively, intra- and interassay CVs less than 10%. The analytical characteristics of the assays were comparable with those of the corresponding single-analyte fluoroimmunoassays performed in microtitration wells, proving the ability of the proposed immunosensor for reliable multianalyte determinations. Moreover, the combination of low-cost disposable plastic capillary tubes with the low consumption of reagents, the short assay time, and the multianalyte feature of the proposed immunosensor indicates its potential for environmental analysis.

show abstract

“…Several reviews have been published on drug testing in forensic toxicology (436-439). Drugs of abuse have been detected in urine using TLC (440,441), immunoassays (442)(443)(444)(445) including EMIT (446-449), EMIT and GC/MS (450), EMIT and RIA (451, 452), SPE and HPLC with fluorescence detection (453), SPE and LC/MS (454), HPLC-DAD (455), online immunoaffinity chromatography/HPLC/MS (456), GC/MS (457), and GC/MS, TLC/GC, and EMIT (458). The Triage Panel for Drugs of Abuse has been evaluated for detection of drugs in urine (459)(460)(461).…”

mentioning

confidence: 99%