Multi-well platform manufacturing combining stereolithography and pulsed laser ablation for cellular studies

Abstract

Novel cell culture platforms, with more physiological surface roughness, require different technologies capable of precisely micropattern substrates. 3D printing offers a considerable accuracy and user-friendly procedures. For its part, pulsed laser ablation proves to be a versatile technology to perform detailed surface micropatterning. In this work, both technologies were combined to easily fabricate a versatile PDMS multi-well platform for performing cellular studies on a micropatterned biocompatible surface.