2015
DOI: 10.1186/s12951-015-0152-y
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Multi-walled carbon nanotubes change morpho-functional and GABA characteristics of mouse cortical astrocytes

Abstract: BackgroundMulti-walled carbon nanotubes (MW-CNTs) have been extensively explored for their possible beneficial use in the nervous system. CNTs have shown to modulate neuronal growth and electrical properties, but its effect that varying length of MW-CNTs on primary astrocyte roles have not been clearly demonstrated yet.ResultsWe investigate here the effect of MW-CNTs on astrocytic morphology, cell–cell interaction and the distribution of intracellular GABA (gamma-amino butyric acid). Primary cultured cortical … Show more

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Cited by 9 publications
(13 citation statements)
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“…a-MWCNTs 2 were also found to internalize in GABAergic glial cells (GAD + /GFAP + cells) in both healthy and diabetic rats ( Figure 6B). This result, indicating that almost exclusively GABAergic astrocytes and neurons are able to uptake MWCNTs in vivo after their intranasal delivery, corroborate the previously reported in vitro evidences of MWCNTs internalization in mouse cortical GABAergic astrocytes 43 . Moreover, although percentage of total GAD + cells did not vary neither after diabetes induction nor after treatment with both MWCNTs ( Figure 6C), the percentage of GAD + cells that internalized a-MWCNTs 2 was significantly decreased in treated diabetic rats as compared to treated healthy rats ( Figure 4D).…”
Section: Selective Cellular Uptake Of Cnts By Neurons and Gliasupporting
confidence: 92%
“…a-MWCNTs 2 were also found to internalize in GABAergic glial cells (GAD + /GFAP + cells) in both healthy and diabetic rats ( Figure 6B). This result, indicating that almost exclusively GABAergic astrocytes and neurons are able to uptake MWCNTs in vivo after their intranasal delivery, corroborate the previously reported in vitro evidences of MWCNTs internalization in mouse cortical GABAergic astrocytes 43 . Moreover, although percentage of total GAD + cells did not vary neither after diabetes induction nor after treatment with both MWCNTs ( Figure 6C), the percentage of GAD + cells that internalized a-MWCNTs 2 was significantly decreased in treated diabetic rats as compared to treated healthy rats ( Figure 4D).…”
Section: Selective Cellular Uptake Of Cnts By Neurons and Gliasupporting
confidence: 92%
“…Astrocytes can form gap junctions with neighboring cells, thereby forming interconnected groups of cells sharing a common cytoplasm [26]. We suggest that astrocytes on MW-CNTs (50 nm, 1,000 nm) have more cell–cell interaction ( p  < 0.05), with higher numbers of cell processes compared with those seen on PDL coverslips [16]. Some reports show that the cause of neurodegenerative diseases, such as Parkinson’s disease and AD, is a progressive loss of structure or function of neurons, as well as neuronal cell death.…”
Section: Cnts Modulate Morphology Of Glial Cellsmentioning
confidence: 99%
“…Based on these studies, we discuss functionalized CNTs which can implant in the brain for brain disease therapy and modulate GABA. We investigated intracellular GABA distribution on MW-CNT and PDL coverslips (Figure 1) [16]. PDL is a positively charged material which is generally used to attach cells on to culture dishes and used as a control group.…”
Section: Cnts Modulate the Intracellular Distribution Of Gaba In Astrmentioning
confidence: 99%
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“…Even gold nanoparticles, widely considered to exhibit little toxicity and high biocompatibility, can induce neurotoxic effects (Jung et al, 2014), which emphasizes the need for rigorous studies evaluating the potential neurotoxicity of nanomaterials. The cytotoxicity due to CNT exposure has been evaluated in various brain cell types including microglia,(Bardi et al, 2013; Bussy et al, 2015) astrocytes(Meng et al, 2013; Min et al, 2015; Zhang et al, 2011) and neurons(Bussy et al, 2015; Lee et al, 2011). The outcomes of these studies vary widely with some research indicating that CNTs are generally safe while others find CNTs to be cytotoxic and induce inflammation.…”
Section: Introductionmentioning
confidence: 99%