Multi-targeted B and Co co-doped 45S5 bioactive glasses with angiogenic potential for bone regeneration

Chen, Si; Michálek, Martin; Galusková, Dagmar; Michalková, Monika; Švančárek, Peter; Talimian, Ali; Kaňková, Hana; Kraxner, Jozef; Zheng, Kai; Liverani, Liliana; Galusek, Dušan; Boccaccını, Aldo R.

doi:10.1016/j.msec.2020.110909

Cited by 33 publications

(13 citation statements)

References 41 publications

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“…Vascularization is vital during tissue regeneration [26] and VEGF is an essential cytokine during angiogenesis [59], one of the vascularization mechanisms [60]. The results of the ELISA test showed that, similar to our previous research results [33], the sample with a high content of B and Co (PCL-B10Co4) significantly promoted the secretion of VEGF in comparison to samples with either no or low B and Co content (PCL-B0Co0 and PCL-B5Co2). On the other hand, few studies have reported ion release data from electrospun fiber mats during cell culture.…”

Section: Discussionsupporting

confidence: 80%

“…The VEGF secretion of cells seeded on PCL-B10Co4 was higher than that on PCL-B0Co0 and PCL-B5Co2. To properly evaluate the results obtained from total VEGF secretion, it is possible to analyze the absorbance ratio of VEGF and WST-8, which reflects the secretion of VEGF per unit number of cells [ 33 ], and it is shown in Figure 7 b. The PCL-B10Co4 sample showed the highest secretion of VEGF per unit number of cells.…”

Section: Resultsmentioning

confidence: 99%

“…Boldbaatar et al fabricated SiO 2 -CoO glass microspheres and reported the synergistic effect of Si and Co dual ion release on the promotion of angiogenesis [32]. In our previous study, multi-targeted B and Co co-doped bioactive glasses based on 45S5 bioactive glass prepared by the melt quenching method were investigated, which showed the synergistic effect of the two ions in increasing the secretion of VEGF from bone marrow-derived stromal cells (ST-2 cell line), indicating a potentially positive effect on angiogenesis [33]. Both B and Co have been investigated as therapeutic ions that induce angiogenesis when released from bioactive glasses [34][35][36].…”

Section: Introductionmentioning

confidence: 99%

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Electrospun PCL Fiber Mats Incorporating Multi-Targeted B and Co Co-Doped Bioactive Glass Nanoparticles for Angiogenesis

Chen

Galusková²,

Kaňková³

et al. 2020

Materials

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Vascularization is necessary in tissue engineering to keep adequate blood supply in order to maintain the survival and growth of new tissue. The synergy of biologically active ions with multi-target activity may lead to superior angiogenesis promotion in comparison to single-target approaches but it has been rarely investigated. In this study, polycaprolactone (PCL) fiber mats embedded with B and Co co-doped bioactive glass nanoparticles (BCo.BGNs) were fabricated as a tissue regeneration scaffold designed for promoting angiogenesis. BCo.NBGs were successfully prepared with well-defined spherical shape using a sol-gel method. The PCL fiber mats embedding co-doped bioactive glass nanoparticles were fabricated by electrospinning using benign solvents. The Young’s moduli of the nanoparticle containing PCL fiber mats were similar to those of the neat fiber mats and suitable for scaffolds utilized in soft tissue repair approaches. The mats also showed non-cytotoxicity to ST-2 cells. PCL fiber mats containing BCo.BGNs with a relatively high content of B and Co promoted the secretion of vascular endothelial growth factor to a greater extent than PCL fiber mats with a relatively low B and Co contents, which demonstrates the potential of dual ion release (B and Co) from bioactive glasses to enhance angiogenesis in soft tissue engineering.

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Section: Discussionsupporting

confidence: 80%

Section: Resultsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Electrospun PCL Fiber Mats Incorporating Multi-Targeted B and Co Co-Doped Bioactive Glass Nanoparticles for Angiogenesis

Chen

Galusková²,

Kaňková³

et al. 2020

Materials

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“…Meanwhile, the level of VEGF secretion in the cells cultured on the PCL@CMC/LAP‐Co3 scaffold was significantly higher than the PCL@CMC/LAP ( p = 0.003) and PCL@CMC/LAP‐Co1 ( p = 0.044) scaffolds. Similarly, Chen et al 43 reported that the Co 2+ ‐doped 45S5 bioactive glasses enhanced the VEGF secretion for BM‐MSCs.…”

Section: Resultsmentioning

confidence: 82%

Immobilization of cobalt‐loaded laponite/carboxymethyl chitosan on polycaprolactone nanofiber for improving osteogenesis and angiogenesis activities

Arab‐Ahmadi

Irani

Bakhshi

et al. 2021

Polymers for Advanced Techs

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Designing scaffolds with therapeutic potential to stimulate angiogenesis and osteogenesis is considered a promising strategy in the regeneration of large skeletal defects. In this study, the cobalt ions (Co2+) were incorporated in laponite (LAP) nanodiscs (0.65, 1.3, and 2.6 g/L) and, in combination with carboxymethyl chitosan (CMC), were immobilized on the electrospun polycaprolactone (PCL) nanofiber to fabricate scaffolds for simultaneous enhancing osteogenesis and angiogenesis inductions to human bone marrow mesenchymal stem cells. The Fourier transform infrared, energy dispersive X‐ray, and inductively coupled plasma atomic emission spectrometries confirmed the Co2+‐loading on the LAP nanodiscs and the chemical immobilization on the surface of PCL nanofibers. The result of MTT assay, scanning electron microscopy, and fluorescence staining displayed that Co2+‐loaded scaffolds were biocompatible. Furthermore, the incorporation of Co2+ ions into LAP nanodiscs had significant effects on the calcium deposition and expression of osteogenic markers, alkaline phosphatase and osteonectin, as well as, the expression of angiogenic markers, hypoxia‐inducible factor‐1α and vascular endothelial growth factor of the cells seeded on the scaffolds (p ≤ 0.05). These results suggested that the combination of Co2+ ions with CMC/LAP could result in a synergetic effect on the osteogenesis and angiogenesis stimulations.

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“…Further aspects impacting the primary biocompatibility of a setting are the BG-morphology (e.g., particle size and surface area) and -composition that are leading to altered ion release kinetics [ 16 , 18 , 19 , 37 , 43 , 44 ]. In this study, the concentration of ions released to the CCM has not been determined, as the dissolution characteristics of the 45S5-BG are well known [ 16 , 19 , 45 , 46 , 47 ]. An attractive possibility to evaluate the specific influence of ionic dissolution products is a setting including ICP-OES analysis and an indirect culturing approach, where cells are exposed to dissolution products instead of direct contact to the BG [ 15 , 16 , 48 ].…”

Section: Discussionmentioning

confidence: 99%

Preconditioning of Bioactive Glasses before Introduction to Static Cell Culture: What Is Really Necessary?

Hohenbild

Arango‐Ospina

Moghaddam

et al. 2020

MPs

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Due to their high bioreactivity, the in-vitro analysis of bioactive glasses (BGs) can be challenging when it comes to maintaining a physiological pH. To improve BG biocompatibility, a heterogenic spectrum of preconditioning approaches, such as “passivation” of the BGs by incubation in cell culture medium, are used but have never been directly compared. In this study, the effect of passivation periods of up to 72 h on pH alkalization and viability of human bone marrow-derived mesenchymal stromal cells was evaluated to determine a time-efficient passivation protocol using granules based on the 45S5-BG composition (in wt%: 45.0 SiO2, 24.5 Na2O, 24.5 CaO, 6.0 P2O5) in different concentrations. pH alkalization was most reduced after passivation of 24 h. Cell viability continuously improved with increasing passivation time being significantly higher after passivation of at least 24 h compared to non-passivated 45S5-BG and the necessary passivation time increased with increasing BG concentrations. In this setting, a passivation period of 24 h presented as an effective approach to provide a biocompatible cell culture setting. In conclusion, before introduction of BGs in cell culture, different passivation periods should be evaluated in order to meet the respective experimental settings, e.g., by following the experimental protocols used in this study.

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Multi-targeted B and Co co-doped 45S5 bioactive glasses with angiogenic potential for bone regeneration

Cited by 33 publications

References 41 publications

Electrospun PCL Fiber Mats Incorporating Multi-Targeted B and Co Co-Doped Bioactive Glass Nanoparticles for Angiogenesis

Electrospun PCL Fiber Mats Incorporating Multi-Targeted B and Co Co-Doped Bioactive Glass Nanoparticles for Angiogenesis

Immobilization of cobalt‐loaded laponite/carboxymethyl chitosan on polycaprolactone nanofiber for improving osteogenesis and angiogenesis activities

Preconditioning of Bioactive Glasses before Introduction to Static Cell Culture: What Is Really Necessary?

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