Multi-stress tolerant plant growth promoting Pseudomonas spp. MCC 3145 producing cytostatic and fungicidal pigment

Patil, Sandeep; Nikam, Monali; Anokhina, Tatyana S.; Кочетков, В. В.; Chaudhari, Ambalal

doi:10.1016/j.bcab.2017.02.006

Cited by 18 publications

(5 citation statements)

References 49 publications

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“…MCC 3145 for wich the inoculation stimulated the germination of mung bean ( Vigna radiata ) seeds and increased root length with a marked tolerance against agricultural contaminants such as metals, salt, pesticides, and herbicides. Additionally, this organism produced phenazine-1-carboxylic acid (PCA) with fungicidal (against phytopathogens) and anticancer activities ( Patil et al, 2017 )…”

Section: Bacteria- Plant Interactions In the Rhizospherementioning

confidence: 99%

Is —— the rhizosphere a source of applicable multi-beneficial microorganisms for plant enhancement?

Chamkhi

Omari

Balahbib

et al. 2022

Saudi Journal of Biological Sciences

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Section: Bacteria- Plant Interactions In the Rhizospherementioning

confidence: 99%

Is —— the rhizosphere a source of applicable multi-beneficial microorganisms for plant enhancement?

Chamkhi

Omari

Balahbib

et al. 2022

Saudi Journal of Biological Sciences

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“…Even though both extracts were effective, the pyocyanin extracted from the clinical source was more potent than that from the environmental isolate [ 31 ]. In a prior experimental study, Patil et al [ 72 ] found that pyocyanin’s concentrations required to inhibit cell proliferation varied when applied to different cell lines, indicating that the anticancer impact of pyocyanin is type-specific. This variation has been linked to differences in physiology, metabolism, and genetic makeup between cell lines [ 19 , 72 ].…”

Section: Discussionmentioning

confidence: 99%

Transforming microbial pigment into therapeutic revelation: extraction and characterization of pyocyanin from Pseudomonas aeruginosa and its therapeutic potential as an antibacterial and anticancer agent

Marey,

Abozahra,

El-Nikhely

et al. 2024

Microb Cell Fact

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Background The objectives of the current study were to extract pyocyanin from Pseudomonas aeruginosa clinical isolates, characterize its chemical nature, and assess its biological activity against different bacteria and cancer cells. Due to its diverse bioactive properties, pyocyanin, being one of the virulence factors of P. aeruginosa, holds a promising, safe, and available therapeutic potential. Methods 30 clinical P. aeruginosa isolates were collected from different sources of infections and identified by routine methods, the VITEK 2 compact system, and 16 S rRNA. The phenazine-modifying genes (phzM, phzS) were identified using polymerase chain reaction (PCR). Pyocyanin chemical characterization included UV-Vis spectrophotometry, Fourier Transform Infra-Red spectroscopy (FTIR), Gas Chromatography-Mass Spectrometry (GC-MS), and Liquid Chromatography-Mass Spectrometry (LC-MS). The biological activity of pyocyanin was explored by determining the MIC values against different clinical bacterial strains and assessing its anticancer activity against A549, MDA-MB-231, and Caco-2 cancer cell lines using cytotoxicity, wound healing and colony forming assays. Results All identified isolates harboured at least one of the phzM or phzS genes. The co-presence of both genes was demonstrated in 13 isolates. The UV-VIS absorbance peaks were maxima at 215, 265, 385, and 520 nm. FTIR could identify the characteristic pyocyanin functional groups, whereas both GC-MS and LC-MS elucidated the chemical formula C11H18N2O2, with a molecular weight 210. The quadri-technical analytical approaches confirmed the chemical nature of the extracted pyocyanin. The extract showed broad-spectrum antibacterial activity, with the greatest activity against Bacillus, Staphylococcus, and Streptococcus species (MICs 31.25–125 µg/mL), followed by E. coli isolates (MICs 250–1000 µg/mL). Regarding the anticancer activity, the pyocyanin extract showed IC50 values against A549, MDA-MB-231, and Caco-2 cancer cell lines of 130, 105, and 187.9 µg/mL, respectively. Furthermore, pyocyanin has markedly suppressed colony formation and migratory abilities in these cells. Conclusions The extracted pyocyanin has demonstrated to be a potentially effective candidate against various bacterial infections and cancers. Hence, the current findings could contribute to producing this natural compound easily through an affordable method. Nonetheless, future studies are required to investigate pyocyanin’s effects in vivo and analyse the results of combining it with other traditional antibiotics or anticancer drugs.

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“…The anticancer effect of pyocyanin also varies based on the type of cancer as shown by Patil et al ( 2017 ) in an experimental study wherein the concentration of pyocyanin required to completely inhibit cell growth was 160.29 μM in the case of A549 human lung carcinoma cells while a concentration of 269.22 μM was required for HeLa human cervical cancer cells. For KB human squamous cell carcinoma cells, only concentrations higher than 380.52 μM were able to inhibit cell proliferation.…”

Section: Biomedical Applications Of Pyocyaninmentioning

confidence: 99%

A biomedical perspective of pyocyanin from Pseudomonas aeruginosa: its applications and challenges

Mudaliar,

Bharath Prasad

2024

World J Microbiol Biotechnol

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Pyocyanin is a bioactive pigment produced by Pseudomonas aeruginosa. It is an important virulence factor that plays a critical role in P. aeruginosa infections as a redox-active secondary metabolite and a quorum sensing (QS) signaling molecule. Pyocyanin production from chorismic acid requires the involvement of two homologous operons, phz1 and phz2, which are activated by QS regulatory proteins. Pyocyanin inhibits the proliferation of bacterial, fungal, and mammalian cells by inducing oxidative stress due to which it acts as a potent antibacterial, antifungal, and anticancer agent. Its potential role as a neuroprotectant needs further exploration. However, pyocyanin exacerbates the damaging effects of nosocomial infections caused by P. aeruginosa in immunocompromised individuals. Further, cystic fibrosis (CF) patients are highly susceptible to persistent P. aeruginosa infections in the respiratory system. The bacterial cells form colonies and three interconnected QS networks—pqs, las, and rhl—get activated, thus stimulating the cells to produce pyocyanin which exacerbates pulmonary complications. As an opportunistic pathogen, P. aeruginosa produces pyocyanin to impede the recovery of injuries like burn wounds through its anti-proliferative activity. Moreover, pyocyanin plays a vital role in compounding P. aeruginosa infections by promoting biofilm formation. This review begins with a brief description of the characteristics of pyocyanin, its activity, and the different aspects of its production including its biosynthesis, the role of QS, and the effect of environmental factors. It then goes on to explore the potential applications of pyocyanin as a biotherapeutic molecule while also highlighting the biomedical challenges and limitations that it presents. Graphical abstract

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Multi-stress tolerant plant growth promoting Pseudomonas spp. MCC 3145 producing cytostatic and fungicidal pigment

Cited by 18 publications

References 49 publications

Is —— the rhizosphere a source of applicable multi-beneficial microorganisms for plant enhancement?

Is —— the rhizosphere a source of applicable multi-beneficial microorganisms for plant enhancement?

Transforming microbial pigment into therapeutic revelation: extraction and characterization of pyocyanin from Pseudomonas aeruginosa and its therapeutic potential as an antibacterial and anticancer agent

A biomedical perspective of pyocyanin from Pseudomonas aeruginosa: its applications and challenges

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