Multi-step dielectrophoresis for separation of particles

Aldaeus, Fredrik; Lin, Yuan; Amberg, Gustav; Roeraade, Johan

doi:10.1016/j.chroma.2006.07.022

Cited by 36 publications

(26 citation statements)

References 16 publications

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“…DEP forces are caused by differences in the electrical properties of the particles relative to the fluid; thus, particles with different sizes, shapes, or electrical properties will experience different forces, allowing DEP to be successfully employed to sort both synthetic and biological particles (e.g., Aldaeus et al 2006;Cen et al 2004;Chen et al 2007;Wu et al 2005). DEP forces depend on both the magnitude and direction of the electric field gradient, captured by the real and imaginary components of the Clausius-Mosotti factor (see Morgan and Green 2003).…”

Section: Introduction To Electrokinetic Phenomenamentioning

confidence: 99%

Phytolith assaying using a micron-scale electrokinetic sorting ring

Stanley

Robertson

d’Entremont

et al. 2011

Archaeol Anthropol Sci

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A particularly useful indicator of past vegetation are phytoliths, glassy products of plant metabolism which have distinctive size and morphology based on the plant taxa which produced them; however, their analysis is a time-consuming task. Building on investigations into mobilizing and sorting of synthetic polystyrene microspheres using a closed loop rectangular microelectromechanical systems (MEMS) electrokinetic array, we investigate these devices' utility for sorting natural microparticles. Using phytolith samples extracted from archaeological sediment, we show that closed loop MEMS can separate the glassy bodies from both mineral contaminants and each other. Also, small differences in polarization between the phytoliths cause lateral segregation between particles of different sizes and shapes. This process facilitates manual analysis by providing a motive force to the phytoliths that translates and rotates them, showing different configurations as they are propelled by the MEMS array. The organization of the phytoliths into streams also opens the door to automated analysis using image processing. Phytolith assaying is therefore feasible using a MEMS electrokinetic ring and merits additional research to explore the potential of this innovative approach.

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Section: Introduction To Electrokinetic Phenomenamentioning

confidence: 99%

Phytolith assaying using a micron-scale electrokinetic sorting ring

Stanley

Robertson

d’Entremont

et al. 2011

Archaeol Anthropol Sci

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“…DEP has been used to distinguish viable from nonviable cells , bacteria , malaria‐infected cells , leukocytes from RBCs , yeast cells , virus , DNA , and proteins . It has also been used to separate or characterize several nonbiological particles, such as polystyrene particles and colloids . Currently, DEP has been able to separate one tumor cell in a mixture of 10 5 normal healthy cells .…”

Section: Methodsmentioning

confidence: 99%

Dielectrophoretic separation of bioparticles in microdevices: A review

2014

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In recent years, dielectrophoretic force has been used to manipulate colloids, inert particles, and biological microparticles, such as red blood cells, white blood cells, platelets, cancer cells, bacteria, yeast, microorganisms, proteins, DNA, etc. This specific electrokinetic technique has been used for trapping, sorting, focusing, filtration, patterning, assembly, and separating biological entities/particles suspended in a buffer medium. Dielectrophoretic forces acting on particles depend on various parameters, for example, charge of the particle, geometry of the device, dielectric constant of the medium and particle, and physiology of the particle. Therefore, to design an effective micro-/nanofluidic separation platform, it is necessary to understand the role of the aforementioned parameters on particle motion. In this paper, we review studies particularly related to dielectrophoretic separation in microfluidic devices. Both experimental and theoretical works by several researchers are highlighted in this article covering AC and DC DEP. In addition, AC/DC DEP, which uses a combination of low frequency AC and DC voltage to manipulate bioparticles, has been discussed briefly. Contactless DEP, a variation of DC DEP in which electrodes do not come in contact with particles, has also been reviewed. Moreover, dielectrophoretic force-based field flow fractionations are featured to demonstrate the bioparticle separation in microfluidic device. In numerical front, a comprehensive review is provided starting from the most simplified effective moment Stokes-drag (EMSD) method to the most advanced interface resolved method. Unlike EMSD method, recently developed advanced numerical methods consider the size and shape of the particle in the electric and flow field calculations, and these methods provide much more accurate results than the EMSD method for microparticles.

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“…Using this concept, various on-chip separation function for particles or cells has been achieved with 2D planar electrodes fabricated at the bottom of the channel, such as global electrodes with different shape and layout (Doh and Cho 2005;Nieuwenhuis et al 2005;Bligh et al 2008), trapezoidal electrode array Lin and Yeow 2007), and slim interdigitated electrode array (Kralj et al 2006;Aldaeus et al 2006;Li et al 2007;Kim et al 2007;Vahey and Voldman 2008;Pommer et al 2008;Han and Frazier 2008;Vykoukal et al 2008). Some other researchers implemented 3D electrode arrays using more Kentsch et al (2003) and Dürr et al (2003) developed microfluidic devices with 3D microelectrodes pairs on both top and bottom sides of the microchannels as deflector structures to separate the micron and submicron particles according to their size.…”

Section: Separationmentioning

confidence: 99%

Electrokinetic motion of particles and cells in microchannels

Kang

2009

Microfluid Nanofluid

186

135

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This paper provides an overview of the electrokinetic phenomena associated with particles and cells in microchannel systems. The most important phenomena covered include electrophoresis, dielectrophoresis, and induced-charge electrokinetics. The latest development of these electrokinetic techniques for particle or cell manipulations in microfluidic systems is reviewed, in terms of the basic theories, mathematical models, numerical and experimental methods, and the key results/findings from the published literatures in the most recent decades. Some of the limitations associated with the negative field effects are discussed and the perspectives for the future investigations are summarized.

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Multi-step dielectrophoresis for separation of particles

Cited by 36 publications

References 16 publications

Phytolith assaying using a micron-scale electrokinetic sorting ring

Phytolith assaying using a micron-scale electrokinetic sorting ring

Dielectrophoretic separation of bioparticles in microdevices: A review

Electrokinetic motion of particles and cells in microchannels

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