Multi‐Scale Lipid Membrane Flow by Electron Beam‐Induced Electrowetting

Miyazako, Hiroki; Mabuchi, Kunihiko; Hoshino, Takayuki

doi:10.1002/admi.202100257

Cited by 3 publications

(1 citation statement)

References 52 publications

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“…18,22) By using this spatiotemporal virtual cathode (VC) tool, various real-time reversible controls of biomaterials have been demonstrated. [23][24][25][26][27] These studies suggested that the VC tool has the potential to manipulate molecular reactions and motions without using the mechanical probing electrode.…”

Section: Introductionmentioning

confidence: 99%

Dielectric characteristics of deformable and maneuverable virtual cathode tool displayed by indirect electron beam drawing

Sasaki

HOSHINO

2022

Jpn. J. Appl. Phys.

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Dielectrophoretic manipulations are deft techniques for soft-matter processes. To actuate the target biomolecules more spatiotemporally, the manipulator which can maneuver the adjustable electric field at high speed is required. We have designed a virtual cathode (VC) tool drawn with an electron beam (EB), which is a deformable and maneuverable electrode. In this report, we investigated the electrochemical response of YOYO-1-labeled DNAs by applying the VC tool and evaluated dependency of its dielectric characteristics on pattern frequency. The specific fluorescent bleaching responses we obtained suggested that work lengths and strength of the VC-induced electric field were enhanced as the applied VC pattern has a high pattern frequency. Moreover, we validated the form of the EB-drawing pattern can also affect dielectric characteristics of the VC tool. These results therefore indicate that the VC tool can control the dielectric phenomenon by a well-tuned tool design, which will lead to more flexible manipulations.

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Section: Introductionmentioning

confidence: 99%

Dielectric characteristics of deformable and maneuverable virtual cathode tool displayed by indirect electron beam drawing

Sasaki

HOSHINO

2022

Jpn. J. Appl. Phys.

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Rapid pattern formation in model cell membranes when using an electron beam

Miyazako

Hoshino

2022

Colloids and Surfaces B: Biointerfaces

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Surface-limited reactions for spatial control of kinesin–microtubule motility assays using indirect irradiation of an electron beam

2022

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Gliding of microtubules (MTs) on kinesins has been applied to lab-on-a-chip devices, which enable autonomous transportation and detection of biomolecules in the field of bioengineering. For rapid fabrication and evaluation of the kinesin–MT based devices, optical control techniques have been developed for control of kinesin activity and density; however, use of caged molecules lacks spatial controllability for long-term experiments, and direct irradiations of UV light onto kinesin-coated surfaces are inherently damaging to MTs due to their depth limit since the heights of the kinesin–MT systems are at the tens of a nanometer scale. Considering surface electric fields in electrolytic solutions are shielded at the nanometer scale due to Debye shielding, in this study, we show that fine spatial control of kinesin density and activity is enabled using surface-limited electrochemical reactions induced by indirect irradiations of an electron beam (EB). An EB is indirectly irradiated onto the kinesins through a 100-nm-thick silicon nitride membrane, and the electrons scattered in the membrane can cause localized electrochemical effects to the kinesins. We show that these localized electrochemical effects cause both ablation of kinesins and motility control of kinesin activity by changing the EB acceleration voltage. In particular, the latter is achieved without complete ablation of MTs, though the MTs are indirectly irradiated by the EB. As a demonstration of on-demand control of gliding MTs, we show the accumulation of the MTs on a target area by scanning the EB. The proposed accumulation technique will lead to rapid prototyping of microdevices based on MT–kinesin motility assay systems.

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Multi‐Scale Lipid Membrane Flow by Electron Beam‐Induced Electrowetting

Cited by 3 publications

References 52 publications

Dielectric characteristics of deformable and maneuverable virtual cathode tool displayed by indirect electron beam drawing

Dielectric characteristics of deformable and maneuverable virtual cathode tool displayed by indirect electron beam drawing

Rapid pattern formation in model cell membranes when using an electron beam

Surface-limited reactions for spatial control of kinesin–microtubule motility assays using indirect irradiation of an electron beam

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